Skip to content
1887

Abstract

Previously the heat-stable enterotoxin in and has been detected by suckling mouse assay, a non-specific approach, and by DNA probes, a time-consuming method. This report describes a polymerase chain reaction (PCR) procedure for the detection of the (NAG-ST) and (O1-ST) gene sequences that is rapid and specific, allowing toxin gene molecular characterisation. A total of 34 and isolates was examined for ST and CT genes. The NAG-ST gene sequence was amplified in 13 of 22 non-O1/non-O139 and three of five strains. A new enterotoxin gene sequence pattern was found with I and I restriction endonuclease PCR fragment digestion of two isolates, in addition to the pattern anticipated from the Genbank sequence, and found with the other ST, These results show that ST-PCR detection is useful for the characterisation of and .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-46-5-398
1997-05-01
2025-12-10

Metrics

Loading full text...

Full text loading...

References

  1. Kaper J. B., Fasano A., Trucksis M. Toxins of Vibrio cholerae. In Waschsmuth I. K., Blake P. A., Olsvik O. (eds) Vibrio cholerae and cholera: molecular to global perspectives Washington, DC: ASM Press; 1994145–176
    [Google Scholar]
  2. Morris J. G. Non-Ol group 1 Vibrio cholerae strains not associated with epidemic diseases. In Waschsmuth I. K., Blake P. A., Olsvik O. (eds) Vibrio cholerae and cholera: molecular to global perspectives Washington, DC: ASM Press; 1994103–115
    [Google Scholar]
  3. Takeda T., Peina Y., Ogawa A. Detection of heat-stable enterotoxin in a cholera toxin gene-positive strain of Vibrio cholerae 01. FEMS Microbiol Lett 1991; 80:23–27
    [Google Scholar]
  4. Yuan P., Ogawa A., Ramamurthy T. Vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus Vibrio. World J Microbiol Biotech 1994; 10:59–63
    [Google Scholar]
  5. Mallard K. E., Desmarchelier P. M. Detection of heat-stable enterotoxin genes among Australian Vibrio cholerae 01 strains. FEMS Microbiol Lett 1995; 127:111–115
    [Google Scholar]
  6. Hoge C. W., Sethabutr O., Bodhidatta L., Echeverria P., Robertson D. C., Morris J. G. Use of a synthetic oligonucleotide probe to detect strains of non-serovar 01 Vibrio cholerae carrying the gene for heat-stable enterotoxin (NAG-ST). J Clin Microbiol 1990; 28:1473–1476
    [Google Scholar]
  7. Fields P. I., Popovic T., Wachsmuth K., Olsvik O. Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae 01 strains from the Latin America cholera epidemic. J Clin Microbiol 1992; 30:2118–2121
    [Google Scholar]
  8. Salles C. A., Momen H., Vicente A. C. P., Coelho A. Vibrio cholerae in South America: polymerase chain reaction and zymovar analysis. Trans R Soc Trop Med Hyg 1993; 87:272
    [Google Scholar]
  9. Shangkuan Y. H., Show Y. S., Wang T. M. Multiplex polymerase chain reaction to detect toxigenic Vibrio cholerae and to biotype Vibrio cholerae 01. J Appl Bacteriol 1995; 79:264–273
    [Google Scholar]
  10. Varela P., Pollevick G. D., Rivas M. Direct detection of Vibrio cholerae in stool samples. J Clin Microbiol 1994; 32:1246–1248
    [Google Scholar]
  11. Ogawa A., Kato J.-I., Watanabe H., Nair B. G., Takeda T. Cloning and nucleotide sequence of a heat-stable enterotoxin gene from Vibrio cholerae non-Ol isolated from a patient with traveller’s diarrhea. Infect Immun 1990; 58:3325–329
    [Google Scholar]
  12. Ogawa A., Takeda T. The gene encoding the heat-stable enterotoxin of Vibrio cholerae is flanked by 123-base pair direct repeats. Microbiol Immunol 1993; 37:607–616
    [Google Scholar]
  13. Salles C. A., Momen H. Identification of Vibrio cholerae by enzyme electrophoresis. Trans R Soc Trop Med Hyg 1991; 85:544–547
    [Google Scholar]
  14. Moseley S. L., Hardy J. W., Huq M. I., Echeverria P., Falkow S. Isolation and nucelotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli. Infect Immun 1983; 39:1167–1174
    [Google Scholar]
  15. Aldova E., Laznickova K., Stepankova E., Lietava J. Isolation of nonagglutinable vibrios from an enteritis outbreak in Czechoslovakia. J Infect Dis 1968; 118:25–31
    [Google Scholar]
  16. Morris J. G., Takeda T., Tall B. D. Experimental non-0 group 1 Vibrio cholerae gastroenteritis in humans. J Clin Invest 1990; 85:697–705
    [Google Scholar]
  17. Nair G. B., Takeda Y. Detection of toxins of Vibrio cholerae 01 and non-Ol. In Waschsmuth I. K., Blake P. A., Olsvik O. (eds) Vibrio cholerae and cholera: molecular to global perspectives Washington, DC: ASM Press; 199453–67
    [Google Scholar]
  18. Dalsgaard A., Serichantalergs O., Shimada T., Sethabutr O., Echeverria P. Prevalence of Vibrio cholerae with heat-stable enterotoxin (NAG-ST) and cholera toxin genes; restriction fragment length polymorphisms of NAG-ST genes among V. cholerae O serogroups from a major shrimp production area in Thailand. J Med Microbiol 1995; 43:216–220
    [Google Scholar]
  19. Arita M., Honda T., Miwatani T., Ohmori K., Takao T., Shimonishi Y. Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-Ol serogroup Hakata. Infect Immun 1991; 59:2186–2188
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-46-5-398
Loading
/content/journal/jmm/10.1099/00222615-46-5-398
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error