1887

Abstract

An outbreak of multiresistant Klebsiella pneumoniae has continued in the Grampian Region of Scotland since 1992. The organism, which generally produces an extended-spectrum -lactamase (ESBL), has spread to several hospitals and nursing homes. DNA from 80 possible outbreak isolates was digested with the restriction endonucleases I and I, and the patterns obtained by pulsed-field gel electrophoresis were compared. Restriction patterns of 79 of the isolates were found to be highly similar with both restriction enzymes, whereas one isolate was unrelated. The outbreak isolates were divided into six subtypes with I and 16 subtypes with I. These subtypes were independent of antibiotic susceptibility pattern, date of isolation and ward of origin, but the XbaI subtype did correlate with the I subtype. It was concluded that the Klebsiella isolates of this outbreak were clonally related.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-46-11-921
1997-11-01
2023-02-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/46/11/medmicro-46-11-921.html?itemId=/content/journal/jmm/10.1099/00222615-46-11-921&mimeType=html&fmt=ahah

References

  1. Hobson R. P., MacKenzie F. M., Gould I. M. An outbreak of multiply-resistant Klebsiella pneumoniae in the Grampian region of Scotland. J Hosp Infect 1996; 33:249–262
    [Google Scholar]
  2. Arlet G., Sanson-le Pors M. J., Rouveau M. Outbreak of nosocomial infections due to Klebsiella pneumoniae producing SHV-4 beta-lactamase. Eur J Clin Microbiol Infect Dis 1990; 9:797–803
    [Google Scholar]
  3. Arlet G., Rouveau M., Casin I., Bouvet P. J. M., Lagrange P. H., Philippon A. Molecular epidemiology of Klebsiella pneumo niae strains that produce SHV-4 ß-lactamase and which were isolated in 14 French hospitals. J Clin Microbiol 1994; 32:2553–2558
    [Google Scholar]
  4. Johnson A. P., Weinbren M. J., Ayling-Smith B., Du Bois S. K., Amyes S. G. B., George R. C. Outbreak of infection in two UK hospitals caused by a strain of Klebsiella pneumoniae resistant to cefotaxime and ceftazidime. J Hosp Infect 1992; 20:97–103
    [Google Scholar]
  5. Brun-Buisson C., Legrand P., Philippon A., Montravers F., Ansquer M., Duval J. Transferable enzymatic resistance to third-generation cephalosporins during nosocomial outbreak of multiresistant Klebsiella pneumoniae. Lancet 1987; 2:302–306
    [Google Scholar]
  6. Kitzis M. D., Billot-Klein D., Goldstein F. W. Dissemination of the novel plasmid-mediated ß-lactamase CTX-1, which confers resistance to broad-spectrum cephalosporins, and its inhibition by ß-lactamase inhibitors. Antimicrob Agents Chemother 1988; 32:9–14
    [Google Scholar]
  7. Bingen E. H., Desjardins P., Arlet G. Molecular epidemiology of plasmid spread among extended broad-spectrum ß-lactamase-producing Klebsiella pneumoniae isolates in a pediatric hospital. J Clin Microbiol 1993; 31:179–184
    [Google Scholar]
  8. Poh C. L., Yap S. C., Yeo M. Pulsed-field gel electrophoresis for differentiation of hospital isolates of Klebsiella pneumoniae. J Hosp Infect 1993; 24:123–128
    [Google Scholar]
  9. Gouby A., Neuwirth C., Bourg G. Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum ß-lactamase-producing Klebsiella pneumoniae in a geriatric hospital. J Clin Microbiol 1994; 32:301–305
    [Google Scholar]
  10. National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically. 2nd edn Approved Standard NCCLS Document M7-A2 1990 Villanova, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  11. Jarlier V., Nicolas M. H., Fournier G., Philippon A. Extended broad-spectrum ß-lactamases conferring transferable resistance to newer ß-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis 1988; 10:867–878
    [Google Scholar]
  12. Smith C. L., Econome J. G., Schutt A., Klco S., Cantor C. R. A physical map of the Escherichia coli K12 genome. Science 1987; 236:1448–1453
    [Google Scholar]
  13. Tenover F. C., Arbeit R. D., Goering R. V. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33:2233–2239
    [Google Scholar]
  14. Jacoby G. A., Medeiros A. A. More extended-spectrum ß-lactamases. Antimicrob Agents Chemother 1991; 35:1697–1704
    [Google Scholar]
  15. Thompson W., Romance L., Bialkowska-Hobrazanska H., Rennie R. P., Ashton F., Nicolle L. E. Klebsiella pneumoniae infection on a rehabilitation unit: comparison of epidemiologic typing methods. Infect Control Hosp Epidemiol 1993; 14:203–210
    [Google Scholar]
  16. Jacoby G. A., Han P. Detection of extended-spectrum ß-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli. J Clin Microbiol 1996; 34:908–911
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-46-11-921
Loading
/content/journal/jmm/10.1099/00222615-46-11-921
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error