1887

Abstract

Two monoclonal antibodies (MAbs) to a 36-kDa extracellular metalloprotease (PSCP) from were found to react with thermolysin, elastase, alkaline protease (Apr) and LasA, protease (SMP), protease (AhP), and both the lethal factor (LF) and protective antigen (PA) of on immunoblots. The MAbs were capable of neutralising the proteolytic activity of thermolysin, elastase and PSCP but not that of Apr, SMP, and AhP. These results suggest that these MAbs may be able to differentiate between the thermolysin and serralysin family of metalloproteases on the basis of their neutralisation capability and could, therefore, be useful tools in the characterisation of new bacterial proteases.

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1996-09-01
2022-10-07
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