A rapid PCR-based method for the identification of four species is described. Primers to conserved sequences in the V3 region of large subunit rDNA were used to amplify DNA from and The sequences were aligned and areas of non-concordance were used to design four species-specific forward primers. In a blind study of 82 yeast strains, four PCRs based on these primers correctly identified nine , 18 , 13 and 18 strains after gel electrophoresis of amplified DNA. Furthermore, of 17 other strains (10 species) and seven strains from other yeast genera ( and ) only one false positive amplification product (with ) was seen. These PCRs offer a rapid alternative to conventional techniques for the identification of and


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