1887

Journal of Medical Microbiology logo

Volume 44, Issue 4

An evaluation of pre-poured selective media for the isolation of Free

Abstract

Fourteen commercial media supplied as pre-poured plates were compared with an ‘in-house’ selective medium for their ability to support the growth of 105 gonococcal isolates (representing a wide variety of serovars encountered in natural infection), 25 meningococcal and 20 isolates, and to inhibit the growth of 71 isolates of non-pathogenic neisseriae and miscellaneous organisms. Only two of the prepoured plate media and the in-house selective medium yielded growth of duplicate cultures of all 105 gonococcal isolates after incubation for 24 h: one other medium provided growth of all the isolates after incubation for 48 h. The ability of the various media to suppress the growth of the 71 isolates of non-pathogenic neisseriae and miscellaneous organisms ranged from 97.2 to 71.8% of isolates inhibited. Of the four media that enabled growth of all the gonococcal strains, inhibition was 94.4% for the in-house medium, 85.9% and 80.3% for the two media on which all gonococci grew after 24 h and 71.8% for the medium on which all of the gonococci grew after 48 h. Failure of growth of gonococci was associated with: serogroup IA isolates (p < 0.001), AHU auxotype (p < 0.001) and the presence of vancomycin rather than lincomycin in the selective medium (p < 0.02). The use of 10% blood and a highly nutritious medium based on the original New York City (NYC) or modified New York City (MNYC) formulation were also important in supporting growth of gonococci. One of the main problems in lack of selectivity was a failure to inhibit the growth of yeasts. As effective inhibition of yeasts was obtained with other media containing the same concentration of amphotericin, failure may be due to batch variation of supplement, media preparation, or reduced shelf life of the media. None of the commercially available pre-poured media performed as well as the in-house medium despite the fact that some of the media were prepared to a very similar formula.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© J. MED. MICROBIOL. 1996
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-44-4-253
1996-04-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/44/4/medmicro-44-4-253.html?itemId=/content/journal/jmm/10.1099/00222615-44-4-253&mimeType=html&fmt=ahah

References

  1. Adler M. W. Diagnostic treatment and reporting criteria for gonorrhoea in sexually transmitted disease clinics in England and Wales. 1 Diagnosis. Br J Vener Dis 1978; 54:10–14
     [Google Scholar]
  2. Young H., Reid K. G. Immunological diagnosis of gonococcal infection. In Young H., McMillan A. (eds) Immunological diagnosis of sexually transmitted diseases New York: Marcel Dekker Inc; 198877–116
     [Google Scholar]
  3. Young H. Cultural diagnosis of gonorrhoea with modified New York City (MNYC) medium. Br J Vener Dis 1978; 54:36–40
     [Google Scholar]
  4. Saigh J. H., Sanders C. C., Sanders W. E. Inhibition of Neisseria gonorrhoeae by aerobic and facultatively anaerobic components of the endocervical flora: evidence for a protective effect against infection. Infect Immun 1978; 19:704–710
     [Google Scholar]
  5. Kraus S. J., Geller R. C., Perkins G. H., Rhoden D. L. Interference of Neisseria gonorrhoeae growth by other bacterial species. J Clin Microbiol 1976; 4:288–295
     [Google Scholar]
  6. Kay D., Levinson M. In vitro inhibition of growth of Neisseria gonorrhoeae by growth of genital microorganisms. Sex Transm Dis 1977; 4:1–3
     [Google Scholar]
  7. Shtibel R. Inhibition of growth of N. gonorrhoeae by bacterial interference. Can J Microbiol 1976; 22:1430–1436
     [Google Scholar]
  8. Hipp S. S., Lawton W. D., Chen N. C., Gaafar H. A. Inhibition of Neisseria gonorrhoeae by a factor produced by Candida albicans. Appl Microbiol 1974; 27:192–201
     [Google Scholar]
  9. Hipp S. S., Lawton W. D., Savage M., Gaafar H. A. Selective interaction of Neisseria gonorrhoeae and Candida albicans and its possible role in clinical specimens. J Clin Microbiol 1975; 1:476–77
     [Google Scholar]
  10. Young H., Saraflan S. K., Harris A. B., McMillan A. Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings. J Med Microbiol 1983; 16:183–191
     [Google Scholar]
  11. Reichart C. A., Rupkey L. M., Brady W. E., Hook E. W. Comparison of GC-Lect and modified Thayer–Martin media for isolation of Neisseria gonorrhoeae. J Clin Microbiol 1989; 27:808–811
     [Google Scholar]
  12. Windall J. J., Hall M. M., Washington J. A., Douglass T. J., Weed L. A. Inhibitory effects of vancomycin on Neisseria gonorrhoeae in Thayer-Martin medium. J Infect Dis 1980; 142:775
     [Google Scholar]
  13. Brorson J.-E., Holmberg I., Nygren B., Seeberg S. Vancomycin-sensitive strains of Neisseria gonorrhoeae. A problem for the diagnostic laboratory. Br J Vener Dis 1973; 49:452–453
     [Google Scholar]
  14. Mirrett S., Reller L. B., Knapp J. S. Neisseria gonorrhoeae strains inhibited by vancomycin in selective media and correlation with auxotype. J Clin Microbiol 1981; 14:94–99
     [Google Scholar]
  15. Exner A. C., Shinners E. N., Pace P. J., Catlin B. W. Auxotypes and antibacterial resistance of gonococci with differing susceptibilities to vancomycin. Br J Vener Dis 1982; 58:166–175
     [Google Scholar]
  16. Knapp J. S., Tam M. R., Nowinski R. C., Holmes K. K., Sandstrom E. G. Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. J Infect Dis 1984; 150:44–48
     [Google Scholar]
  17. Kohl P. K., Knapp J. S., Hofinann H. Epidemiological analysis of Neisseria gonorrhoeae in the Federal Republic of Germany by auxotyping and serological classification using monoclonal antibodies. Genitourin Med 1986; 62:145–150
     [Google Scholar]
  18. Moyes A., Young H. Typing of Neisseria gonorrhoeae by auxotype, serovar and lectin agglutination. Br J Biomed Sci 1993; 50:295–300
     [Google Scholar]
  19. Faur Y. C., Weisburd M. H., Wilson M. E., May P. S. A new medium for the isolation of pathogenic Neisseria (NYC medium). I. Formulation and comparisons with standard media. Hlth Lab Sci 1973; 10:44–54
     [Google Scholar]
  20. Faur Y. C., Weisburd M. H., Wilson M. E. The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens. Hlth Lab Sci 1978; 15:22–27
     [Google Scholar]
  21. Odegaard K., Solberg O., Lind J., Myhre G., Nyland B. Lincomycin in selective media for the isolation of Neisseria gonorrhoeae. Acta Pathol Microbiol Scand (B) 1975; 83:301–304
     [Google Scholar]
  22. Hookham A. B. Thayer-Martin medium and modified New York City medium for the cultural diagnosis of gonorrhoea. Br J Vener Dis 1981; 57:213
     [Google Scholar]
  23. Svarva P. L., Maeland J. L. Comparison of two selective media in the cultural diagnosis of gonorrhoea. Acta Pathol Microbiol Scand (B) 1979; 87:391–392
     [Google Scholar]
  24. Granato P. A., Schneible-Smith C., Weiner L. B. Use of New York City medium for improved recovery of Neisseria gonorrhoeae from clinical specimens. J Clin Microbiol 1981; 13:963–968
     [Google Scholar]
  25. Kohl P. K., Pekar U., Hoffmann H., Petzoldt D. Characterization of Neisseria gonorrhoeae isolates from prostitutes with single and multiple infections. In Poolman J. T., Zanen H. C., Meyer T. F. (eds) Gonococci and meningococci Dordrecht: Kluwer Academic Publishers; 1987143–146
     [Google Scholar]
  26. Faur Y. C., Weisburd M. H., Wilson M. E. A new medium for the isolation of pathogenic Neisseria (NYC medium) II. Effect of amphotericin B and trimethoprim lactate on selectivity. Hlth Lab Sci 1973; 10:55–60
     [Google Scholar]
  27. Fallon R. J., Young H. Neisseria: Acinetobacter: Branhamella. In Collee J. G., Duguid J. P., Fraser A. G., Marmion B. P. (eds) Mackie and McCartney Practical medical microbiology Edinburgh: Churchill Livingstone; 1989360–373
     [Google Scholar]
  28. Knapp J. S., Totten P. A., Mulks M. H., Minshew B. M. Characterization of Neisseria cinerea, a nonpathogenic species isolated on Martin-Lewis medium selective for pathogenic Neisseria spp. J Clin Microbiol 1984; 19:63–67
     [Google Scholar]
  29. Boyce J. M., Mitchell E. B. Difficulties in differentiating Neisseria cinerea from Neisseria gonorrhoeae in rapid systems used for identifying pathogenic Neisseria species. J Clin Microbiol 1985; 22:731–734
     [Google Scholar]
  30. Boyce J. M., Mitchell E. B., Knapp J. S., Buttke T. M. Production of 14C-labeled gas in BACTEC Neisseria Differentiation kits by Neisseria cinerea. J Clin Microbiol 1985; 22:416–418
     [Google Scholar]
  31. Lawton W. D., Koch L. W. Comparison of commercially available New York City and Martin-Lewis medium for recovery of Neisseria gonorrhoeae from clinical specimens. J Clin Microbiol 1982; 16:754–755
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-44-4-253
Loading
An evaluation of pre-poured selective media for the isolation of Neisseria gonorrhoeae
J. Med. Microbiol. 44, 253 (1996); https://doi.org/10.1099/00222615-44-4-253
/content/journal/jmm/10.1099/00222615-44-4-253
/content/journal/jmm/10.1099/00222615-44-4-253
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error