1887

Abstract

A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test correlated well with those obtained by the reverse passive latex agglutination test. Thus, this method is convenient and reliable for the detection of in staphylococci, which could be useful for both research and clinical purposes. This method demonstrated that was more prevalent in methicillin-resistant (56%) than in methicillin-susceptible (4%).

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1996-03-01
2024-12-13
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References

  1. Bergdoll M. S., Crass B. A., Reiser R. F., Robbins R. N., Davis J. P. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates. Lancet 1981; 1:1017–1021
    [Google Scholar]
  2. Schlievert P. M., Shands K. N., Dan B. B., Schmid G. P., Nishimura R. D. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis 1981; 143:509–516
    [Google Scholar]
  3. Todd J., Fishaut M., Kapral F., Welch T. Toxic-shock syndrome associated with phage-group-I staphylococci. Lancet 1978; 2:1116–1118
    [Google Scholar]
  4. Marrack P., Kappler J. The staphylococcal enterotoxins and their relatives. Science 1990; 248:705–711
    [Google Scholar]
  5. Norton S. D., Schlievert P. M., Novick R. P., Jenkins M. K. Molecular requirements for T cell activation by the staphylococcal toxic shock syndrome toxin-1. J Immunol 1990; 144:2089–2095
    [Google Scholar]
  6. Schlievert P. M. Role of superantigens in human disease. J Infect Dis 1993; 167:997–1002
    [Google Scholar]
  7. Igarashi H., Fujikawa H., Shingaki M., Bergdoll M. S. Latex agglutination test for staphylococcal toxic shock syndrome toxin 1. J Clin Microbiol 1986; 23:509–512
    [Google Scholar]
  8. Miwa K., Fukuyama M., Kunitomo T., Igarashi H. Rapid assay for detection of toxic shock syndrome toxin 1 from human sera. J Clin Microbiol 1994; 32:539–542
    [Google Scholar]
  9. Wells D. E., Reeves M. W., McKinney R. M. Production and characterization of monoclonal antibodies to toxic shock syndrome toxin 1 and use of a monoclonal antibody in a rapid, one-step enzyme-linked immunosorbent assay for detection of picogram quantities of toxic shock syndrome toxin l. J Clin Microbiol 1987; 25:516–521
    [Google Scholar]
  10. Mills J. T., Dodel A. W., Kass E. H. Regulation of staphylococcal toxic shock syndrome toxin-1 and total exoprotein production by magnesium ion. Infect Immun 1986; 53:663–670
    [Google Scholar]
  11. Schlievert P. M., Blomster D. A. Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors. J Infect Dis 1983; 147:236–242
    [Google Scholar]
  12. Bonventre P. F., Weckbach L., Harth G., Haidaris C. Distribution and expression of toxic shock syndrome toxin 1 gene among Staphylococcus aureus isolates of toxic shock syndrome and non-toxic shock syndrome origin. Rev Infect Dis 1989; 11: Supplement 1S90–S95
    [Google Scholar]
  13. Jaulhac B., Bes M., Bomstein N., Piémont Y., Bum Y., Fleurette J. Synthetic DNA probes for detection of genes for enterotoxins A, B, C, D, E, and for TSST-1 in staphylococcal strains. J Appl Bacteriol 1992; 72:386–392
    [Google Scholar]
  14. Johnson W. M., Tyler S. D., Ewan E. P., Ashton F. E., Pollard D. R., Rozee K. R. Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction. J Clin Microbiol 1991; 29:426–430
    [Google Scholar]
  15. Neill R. J., Fanning G. R., Delahoz F., Wolff R., Gemski P. Oligonucleotide probes for detection and differentiation of Staphylococcus aureus strains containing genes for enterotoxins A, B, qnd C and toxic shock syndrome toxin 1. J Clin Microbiol 1990; 28:1514–1518
    [Google Scholar]
  16. Brumfitt W., Hamilton-Miller J. Methicillin-resistant Staphylococcus aureus . N Engl J Med 1989; 320:1188–1196
    [Google Scholar]
  17. National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. In National Committee for Clinical Laboratory Standards (ed) Approved standard Villanova, PA: 1990M7–A2
    [Google Scholar]
  18. Jablonski E., Moomaw E. W., Tullis R. H., Ruth J. L. Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acid Res 1986; 14:6115–6128
    [Google Scholar]
  19. Blomster-Hautamaa D. A., Kreiswirth B. N., Kornblum J. S., Novick R. P., Schlievert P. M. The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. J Biol Chem 1986; 261:15783–15786
    [Google Scholar]
  20. Yoh M., Miyagi K., Matsumoto Y. Development of an enzyme-labelled oligonucleotide probe for the cholera toxin gene. J Clin Microbiol 1993; 31:1312–1314
    [Google Scholar]
  21. Recsei P., Kreisworth B., O’Reilly M., Schlievert P., Gruss A., Novick R. P. Regulation of exoprotein gene expression in Staphylococcus aureus by agr . Mol Gen Genet 1986; 202:58–61
    [Google Scholar]
  22. Kahler R. C., Boyce J. M., Bergdoll M. S., Lockwood W. R., Taylor M. R. Case report: toxic shock syndrome associated with TSST-1 producing coagulase-negative staphylococci. Am J Med Sci 1986; 292:310–312
    [Google Scholar]
  23. Kreisworth B. N., Schlievert P. M., Novick R. P. Evaluation of coagulase-negative staphylococci for ability to produce toxic shock syndrome toxin 1. J Clin Microbiol 1987; 25:2028–2029
    [Google Scholar]
  24. Abdelnour A., Bremell T., Tarkowski A. Toxic shock syndrome toxin 1 contributes to the arthritogenicity of Staphylococcus aureus . J Infect Dis 1994; 170:94–99
    [Google Scholar]
  25. Takesue Y., Yokoyama T., Kodama T. Toxin involvement in methicillin-resistant Staphylococcus aureus enteritis in gastroenterological surgery. Gastroenterol Jpn 1991; 26:716–720
    [Google Scholar]
  26. Kimura A., Igarashi H., Ushioda H., Okuzumi K., Kobayashi H., Otsuka T. [Epidemiological study of Staphylococcus aureus isolated from the Japanese National University and Medical College Hospitals with coagulase typing, and production of enterotoxins and toxic shock syndrome toxin-1.]. Kausenshogaku Zasshi 1992; 66:1543–1549 (In Japanese with English abstract.)
    [Google Scholar]
  27. Pessione E., Giunta C. Distribution of methicillin-resistance and of protein A among Staphylococcus aureus strains isolated from different hosts. Microbios 1988; 55:147–153
    [Google Scholar]
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