1887

Abstract

Surmmary

The fibronectin-binding proteins of are considered to be important virulence factors for colonisation and infection. The polymerase chain reaction (PCR) was used to detect part of a gene equivalent to the gene of in 120 isolates of staphylococci ( and ). Primers specific for the binding domain region of the gene of produced PCR products of the predicted sizes (93 and 207 bp). The identity of the PCR products was confirmed by digestion with l and nucleic acid hybridisation. The fibronectin-binding activity of the staphylococci was determined with a particle agglutination assay (PAA). The gene was found to be present by PCR in 107 of the 120 staphylococci tested, irrespective of their site of isolation, and expression of the gene was detected by PAA in 101 of the 120 strains.

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1995-02-01
2022-01-24
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