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Volume 41, Issue 5

Characterisation of pathogenic serogroups by pulsed-field gel electrophoresis of genomic | restriction fragments Free

Abstract

Summary

Enteropathogenic is an important cause of human and animal disease. Phenotypic and genotypic characteristics currently used to identify are not necessarily sufficient to differentiate pathogenic from non-pathogenic strains or to analyse the epidemiology of yersiniae at a molecular level. To improve the characterisation of isolates, I restriction fragment length polymorphisms (RFLPs) of chromosomal DNA of more than 100 clinical, animal and environmental isolates were analysed in pulsed-field gel electrophoresis. Highly conserved RFLP patterns with fragments ranging from 15 to 400 kb were detected within each of 10 serogroups tested. Determination of RFLP types makes it possible to discriminate between isolates of different serogroups and other spp. Moreover, Irestriction endonuclease analysis allows even subtyping of strains belonging to a unique serogroup-biotype. Identification of I fragments hybridising with or -homologous sequences was used as an additional discriminating marker. The results indicate that RFLP typing can provide a powerful new tool for the differentiation of clinical isolates.

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Copyright 1994, The Pathological Society of Great Britain and Ireland
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1994-11-01
2024-04-26
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Characterisation of pathogenic Yersinia enterocolitica serogroups by pulsed-field gel electrophoresis of genomic Not| restriction fragments
J. Med. Microbiol. 41, 329 (1994); https://doi.org/10.1099/00222615-41-5-329
/content/journal/jmm/10.1099/00222615-41-5-329
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