1887

Abstract

Summary

pre-exposed to a sub-minimal inhibitory concentration (sub-MIC) of several antibiotics elicits an enhanced humoral response which is protective against challenges with untreated homologous and heterologous bacteria. To characterise the specificity of this response we produced murine monoclonal antibodies (MAbs) to aztreonam-treated O6: K-. This resulted in the identification of MAb MT 1F, of isotype IgG, that recognised a 12-kDa protein component of the untreated bacterial cells. After passive transfer, the MAb displayed protective activity in mice infected with lethal doses of live O6:K- and O111 :B4. In ELISA experiments the MAb cross-reacted with structures located on whole cells of 06: K-, O111: B4, J5 and Re595 and it also exerted a bactericidal activity against live O6: K-. The modifications induced by antibiotic treatment may unmask bacterial epitopes that may elicit the production of MAbs endowed with protective capacity.

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1994-09-01
2024-10-03
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References

  1. Rozenberg-Arska M, Visser MR. Infectious disease therapy in the 1990s. Where are we heading?. Drugs 1992; 43:629–636
    [Google Scholar]
  2. Ziegler EJ, Fischer CJ, Sprung CL. et al. Treatment of Gram-negative bacteraemia and septic shock with HA-1A human monoclonal antibody against endotoxin. A randomized, double-blind, placebo-controlled trial. N Engl J Med 1991; 324:429–436
    [Google Scholar]
  3. McCabe WR, De Maria A, Berberich H, Johns MA. Immunization with rough mutants of Salmonella minnesota: protective activity of IgM and IgG antibody to Re595 (Re Chemotype) mutant. J Infect Dis 1988; 158:291–300
    [Google Scholar]
  4. Raponi G, Keller N, Overbeek BP, Rozenberg-Arska M, Torensma R, Verhoef J. Immunization of mice with antibiotic-treated Escherichia coli results in enhanced protection against challenge with homologous and heterologous bacteria. J Infect Dis 1991; 163:122–127
    [Google Scholar]
  5. Raponi G, Lun MT, Lorino G. et al. Reactivity and protective capacity of a polyclonal antiserum derived from mice immunized with antibiotic exposed Escherichia coli. J Antimicrob Chemother 1993; 31:117–128
    [Google Scholar]
  6. Fazekas De St Groth S, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J Immunol Methods 1980; 35:1–21
    [Google Scholar]
  7. Overbeek BP, Schellekens JFP, Lippe W, Dekker BAT, Verhoef J. Carumonam enhances reactivity of Escherichia coli with mono- and polyclonal antisera to rough mutant Escherichia coli J5. J Clin Microbiol 1987; 25:1009–1013
    [Google Scholar]
  8. Eliasson M, Olsson A, Palmcrantz E. et al. Chimeric IgG-binding receptors engineered from staphylococcal protein A and streptococcal protein G. J Biol Chem 1988; 263:4323–4327
    [Google Scholar]
  9. Schellekens J, Benne CA, De Zeeuw GR, Rozenberg-Arska M, Verhoef J. Natural occurring IgG and IgM antibodies to gram-negative bacteria and lipopolysaccharides in human sera. Serodiagn Immunother Infect Dis 1988; 2:433–444
    [Google Scholar]
  10. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  11. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4354
    [Google Scholar]
  12. Andrews AT. Electrophoresis: theory, techniques, and biochemical and clinical applications. , 2nd edn. Peacocke AR, Harrington WF. (eds) Monographs on physical biochemistry Oxford: Clarendon Press; 1986
    [Google Scholar]
  13. Brodeur BR, Tsang P, Larose Y. Parameters affecting ascites tumour formation in mice and monoclonal antibody production. J Immunol Methods 1984; 71:265–272
    [Google Scholar]
  14. Calandra T, Glauser MP, Schellekens J. Verhoef J and Swiss-Dutch Immunoglobulin Study Group Treatment of gram-negative septic shock with human IgG antibody to Escherichia coli J5: a prospective, double-blind, randomized trial. J Infect Dis 1988; 158:312–319
    [Google Scholar]
  15. Chedid L, Parant M, Parant F, Boyer F. A proposed mechanism for natural immunity to enterobacteria pathogens. J Immunol 1968; 100:292–301
    [Google Scholar]
  16. Ziegler EJ, McCutchan JA, Fierer J. et al. Treatment of gram-negative bacteraemia and shock with human antiserum to a mutant Escherichia coli. N Engl J Med 1982; 307:1225–1230
    [Google Scholar]
  17. Johns M, Skehill A, McCabe WR. Immunization with rough mutants of Salmonella minnesota IV. Protection by antisera to O and rough antigens against endotoxin. J Infect Dis 1983; 147:57–67
    [Google Scholar]
  18. Leying H, Suerbaum S, Kroll HP, Karch H, Opferkuch W. Influence of beta-lactam antibiotics and ciprofloxacin on composition and immunogenicity of Escherichia coli outer membrane. Antimicrob Agents Chemother 1986; 30:475–480
    [Google Scholar]
  19. Zak O, Kradolfer F. Effect of subminimal inhibitory concentrations of antibiotics in experimental infections. Rev Infect Dis 1979; 1:862–879
    [Google Scholar]
  20. Overbeek BP, de Vos NM, Schellekens JFP, Rozenberg-Arska M, Verhoef J. Beta-lactam antibiotics enhance the building of antibodies to outer membrane, non LPS antigenic structures in Enterobacteriaceae. Serodiagn Immunother Infect Dis 1989; 3:175–183
    [Google Scholar]
  21. Hofstra H, van Tol MJD, Dankert J. Cross-reactivity of major outer membrane proteins of Enterobacteriaceae, studied by crossed immunoelectrophoresis. J Bacteriol 1980; 143:328–337
    [Google Scholar]
  22. Màkelà PH, Kuusi N, Nurminen M, Saxen H, Valtonen M. Porins: the major outer membrane proteins of enteric bacteria as protective antigens. Semin Infect Dis 1982; 4:360–365
    [Google Scholar]
  23. Udhaiyakumar V, Muthukkaruppan VR. Protective immunity induced by outer membrane proteins of Salmonella typhimurium in mice. Infect Immun 1987; 55:816–821
    [Google Scholar]
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