1887

Abstract

Summary

Chromosomal fingerprinting of the type strains of serotypes of O-serogroup D1 with the DNA insertion sequence IS generated patterns which were either serotype-specific (e.g., Typhi), or conserved among groups of related serotypes (e.g., Dublin, Rostock and certain phage types of Enteritidis). The number of IS copies varied considerably, and the IS patterns of type strains of serotypes associated with systemic infections in man were specific and suitable for identifying strains within those serotypes. Polymorphism at 16S rRNA gene loci was examined among type strains and 11 16S rRNA gene profiles were characterised. The most prevalent of these was conserved among type strains of 11 serotypes, and the next most prevalent among type strains of nine serotypes; together, they encompassed 15 unique IS profiles. The distribution and mol. wts of plasmids carrying (virulence) genes could be directly related to certain chromosomal genotypes defined by IS patterns. The presence of virulence plasmids in serotypes Lomalinda, Antarctica and Wangata is reported for the first time. Comparison of 16S rRNA gene profiles and IS patterns provides a definition of genotype that is applicable to epidemiological studies of various group D1 serotypes and should prove particularly useful for those lacking plasmid DNA.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-41-2-112
1994-08-01
2024-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/41/2/medmicro-41-2-112.html?itemId=/content/journal/jmm/10.1099/00222615-41-2-112&mimeType=html&fmt=ahah

References

  1. Popoff MY, Bockemühl J, McWhorter-Murlin A. Supplement 1991 (no. 35) to the Kauffmann-White scheme. Res Microbiol 1992; 143:807–811
    [Google Scholar]
  2. Grimont F, Grimont PAD. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Institut Pasteur (Microbiol) 1986; 137B:165–175
    [Google Scholar]
  3. Lam S, Roth JR. IS200: a Salmonella-specific insertion sequence. Cell 1983; 34:951–960
    [Google Scholar]
  4. Lam S, Roth JR. Structural and functional studies of insertion element IS200. J Mol Biol 1986; 187:157–167
    [Google Scholar]
  5. Stanley J, Baquar N, Threlfall EJ. Genotypes and phylogenetic relationships of Salmonella typhimurium are defined by molecular fingerprinting of IS200 and 16S rrn loci. J Gen Microbiol 1993; 139:1133–1140
    [Google Scholar]
  6. Norel F, Coynault C, Miras I, Hermant D, Popoff MY. Cloning and expression of plasmid DNA sequences involved in Salmonella serotype typhimurium virulence. Mol Microbiol 1989; 3:733–743
    [Google Scholar]
  7. Williamson CM, Baird GD, Manning EJ. A common virulence region on plasmids from eleven serotypes of Salmonella. J Gen Microbiol 1988; 134:975–982
    [Google Scholar]
  8. Gulig PA. Virulence plasmids of Salmonella typhimurium and other salmonellae. Microb Pathog 1990; 8:3–11
    [Google Scholar]
  9. Helmuth R, Stephan R, Bunge C, Hoog B, Steinbeck A, Bulling E. Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella types. Infect Immun 1985; 48:175–182
    [Google Scholar]
  10. Roudier C, Krause M, Fierer J, Guiney DG. Correlation between the presence of sequences homologous to the vir region of Salmonella dublin plasmid pSDL2 and the virulence of twenty-two Salmonella serotypes in mice. Infect Immun 1990; 58:1180–1185
    [Google Scholar]
  11. Catalogues of the National Collections of Type Cultures and Pathogenic Fungi, 7th. London: Public Health Laboratory Service; 1989
    [Google Scholar]
  12. Stanley J, Jones CS, Threlfall EJ. Evolutionary lines among Salmonella enteritidis phage types are identified by insertion sequence IS200 distribution. FEMS Microbiol Lett 1991; 82:83–90
    [Google Scholar]
  13. Kado CI, Liu S-T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145:1365–1373
    [Google Scholar]
  14. Wilson K. Preparation of genomic DNA from bacteria. Ausubel FM, Brent R, Kingston RE. et al. (eds) Current protocols in molecular biology, vol 1 New York: Wiley; 19902.4.1–2.4.5
    [Google Scholar]
  15. Gibert I, Carroll K, Hillyard DR, Barbé J, Casadesus J. IS200 is not a member of the IS600 family of insertion sequences. Nucleic Acids Res 1990; 19:1343
    [Google Scholar]
  16. Brosius J, Palmer ML, Kennedy PJ, Noller HF. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 1978; 75:4801–4805
    [Google Scholar]
  17. Ezquerra E, Burnens AP, Frith K, Costas M, Stanley J. Molecular genotype analysis of Salmonella bovismorbificans. Mol Cell Probes 1993; 7:45–54
    [Google Scholar]
  18. Smith NH, Beltran P, Selander RK. Recombination of Salmonella phase 1 flagellin genes generates new serovars. J Bacteriol 1990; 172:2209–2216
    [Google Scholar]
  19. Selander RK, Smith NH, Li J. et al. Molecular evolutionary genetics of the cattle-adapted serovar Salmonella dublin. J Bacteriol 1992; 174:3587–3592
    [Google Scholar]
  20. Li J, Smith NH, Nelson K. et al. Evolutionary origin and radiation of the avian-adapted non-motile salmonellae. J Med Microbiol 1993; 38:129–139
    [Google Scholar]
  21. Anonymous Communicable Disease Report 2 PHLS/CDSC Publications; 1992133149
    [Google Scholar]
  22. Ou JT, Baron LS, Dai XY, Life CA. The virulence plasmids of Salmonella serovars typhimurium, choleraesuis, dublin, and enteritidis, and the cryptic plasmids of Salmonella serovars Copenhagen and sendai belong to the same incompatibility group, but not those of Salmonella serovars durban, gallinarum, give, infantis and pullorum. Microb Pathog 1990; 8:101–107
    [Google Scholar]
  23. Kawahara K, Haragucki Y, Tsuchimoto M, Terakado N, Danbara H. Evidence of correlation between 50-kilobase plasmid of Salmonella choleraesuis and its virulence. Microb Pathog 1988; 4:155–163
    [Google Scholar]
  24. Ørskov F, Ørskov I. Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evolution of the Enterobacteriaceae and other bacteria. J Infect Dis 1983; 148:346–357
    [Google Scholar]
  25. Van Embden JDA, Cave MD, Crawford JT. et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 1993; 31:406–409
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-41-2-112
Loading
/content/journal/jmm/10.1099/00222615-41-2-112
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error