1887

Abstract

Summary

Growth of was inhibited more strongly in continuous flow (CF) culture with -negative faeces of infants than with -positive faeces. Culture of faecal flora of infants yielded a greater variety of bacterial species in -negative than in -positive faeces. In the mixed CF culture of with , , , , and either or isolated from -negative faeces, inhibition of growth of was demonstrated when the pH of the culture medium was decreased. Amino-acid analysis of CF cultures showed considerable utilisation of aspartic acid, serine, threonine, arginine and asparagine. A marked increase in concentrations of citrulline and ornithine was found in the culture that inhibited growth of . The addition of citrulline and ornithine into a Gifu anaerobic medium (GAM) broth produced no inhibition of growth of . The addition of the mixture of the depleted amino acids (aspartic acid, serine, threonine, arginine and asparagine) to the culture filrate or adjustment of the pH of the culture filtrate induced considerable growth of . These results suggest that the inhibition of growth of may be due to consumption of amino acids by intestinal flora, and not to the presence of inhibitors produced by the intestinal flora.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-40-3-179
1994-03-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/40/3/medmicro-40-3-179.html?itemId=/content/journal/jmm/10.1099/00222615-40-3-179&mimeType=html&fmt=ahah

References

  1. Fekety R, Silva J, Toshniwal R. Antibiotic associated colitis: effects of antibiotics on Clostridium difficile and the disease in hamsters. Rev Infect Dis 1979; 1:386–396
    [Google Scholar]
  2. Wilson KH, Silva J, Fekety FR. Suppression of Clostridium difficile by normal hamster cecal flora and prevention of antibiotic-associated cecitis. Infect Immun 1981; 34:626–628
    [Google Scholar]
  3. Lyerly DM, Krivan HC, Wilkins TD. Clostridium difficile its disease and toxins. Clin Microbiol Rev 1988; 1:1–18
    [Google Scholar]
  4. Borriello SP, Barclay FE. Protection of hamsters against Clostridium difficile ileocaecitis by prior colonisation with non-pathogenic strains. J Med Microbiol 1985; 19:339–350
    [Google Scholar]
  5. Wilson KH, Freter R. Interaction of Clostridium difficile and Escherichia coli with microfloras in continuous-flow cultures and gnotobiotic mice. Infect Immun 1986; 54:354–358
    [Google Scholar]
  6. Wilson KH, Sheagren JN. Antagonism of toxigenic Clostridium difficile by nontoxigenic C. difficile . J Infect Dis 1983; 147:733–736
    [Google Scholar]
  7. Borriello SP, Barclay FE. An in-vitro model of colonisation resistance to Clostridium difficile infection. J Med Microbiol 1986; 21:299–309
    [Google Scholar]
  8. Wilson KH, Perini F. Role of competition for nutrients in suppression of Clostridium difficile by the colonie micro-flora. Infect Immun 1988; 56:2610–2614
    [Google Scholar]
  9. Gorbach SL, Chang T-W, Goldin B. Successful treatment of relapsing Clostridium difficile colitis with Lactobacillus GG. Lancet 1987; 2:1519
    [Google Scholar]
  10. Viscidi R, Wiley S, Bartlett JG. Isolation rates and toxigenic potential of Clostridium difficile isolates from various patient populations. Gastroenterology 1981; 81:5–9
    [Google Scholar]
  11. Larson HE, Barclay FE, Honour P, Hill ID. Epidemiology of Clostridium difficile in infants. J Infect Dis 1982; 146:727–733
    [Google Scholar]
  12. Stark PL, Lee A, Parsonage BD. Colonization of the large bowel by Clostridium difficile in healthy infants: quantitative study. Infect Immun 1982; 35:895–899
    [Google Scholar]
  13. Libby JM, Donta ST, Wilkins TD. Clostridium difficile toxin A in infants. J Infect Dis 1983; 148:606
    [Google Scholar]
  14. Al-Jumaili IJ, Shibley M, Lishmann AH, Record CO. Incidence and origin of Clostridium difficile in neonates. J Clin Microbiol 1984; 19:77–78
    [Google Scholar]
  15. Krivan HC, Clark GF, Smith DF, Wilkins TD. Cell surface binding site for Clostridium difficile enterotoxin : Evidence for a glycoconjugate containing the sequence Gala l-3Galβ 1-4GlcNAc. Infect Immun 1986; 53:573–581
    [Google Scholar]
  16. Nakamura S, Mikawa M, Nakashio S. Isolation of Clostridium difficile from the feces of the antibody in sera of young and elderly adults. Microbiol Immunol 1981; 25:345–351
    [Google Scholar]
  17. Rolfe RD, Helebian S, Finegold SM. Bacterial interference between Clostridium difficile and normal fecal flora. J Infect Dis 1981; 143:470–475
    [Google Scholar]
  18. Malamou-Ladas H, Tabaqchali S. Inhibition of Clostridium difficile by faecal streptococci. J Med Microbiol 1982; 15:569–574
    [Google Scholar]
  19. Borriello SP, Barclay FE. In vitro inhibition of C. difficile . Eur J Chemother Antibiot 1982; 2:155–156
    [Google Scholar]
  20. Freter R, Stauffer E, Cleven D, Holdeman LV, Moore WEC. Continuous-flow cultures as in vitro models of the ecology of large intestinal flora. Infect Immun 1983; 39:666–675
    [Google Scholar]
  21. Rolfe RD. Role of volatile fatty acids in colonization resistance to Clostridium difficile . Infect Immun 1984; 45:185–191
    [Google Scholar]
  22. Su WJ, Waechter MJ, Bourlioux P, Dolegeal M, Fourniat J, Mahuzier G. Role of volatile fatty acids in colonization resistance to Clostridium difficile in gnotobiotic mice. Infect Immun 1987; 55:1686–1691
    [Google Scholar]
  23. Freter R, Brickner H, Botney M, Cleven D, Aranki A. Mechanisms that control bacterial populations in continuous-flow culture models of mouse large intestinal flora. Infect Immun 1983; 39:676–685
    [Google Scholar]
  24. Yamamoto T, Takahashi Y, Aiba Y, Ohnishi N, Ozawa A. Effect of Streptococcus parvulus and Peptostreptococcus magnus on cytotoxin levels of Clostridium difficile in anaerobic continuous flow culture. Microbiol Immunol 1987; 31:949–958
    [Google Scholar]
  25. Haslam SC, Ketley JM, Mitchell TJ, Stephen J, Burdon DW, Candy DCA. Growth of Clostridium difficile and production of toxins A and B in complex and defined media. J Med Microbiol 1986; 21:293–297
    [Google Scholar]
  26. Seal D, Borriello SP, Barclay F, Welch A, Piper M, Bonnycastle M. Treatment of relapsing Clostridium difficile diarrhoea by administration of a non-toxigenic strain. Eur J Clin Microbiol 1987; 6:51–53
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-40-3-179
Loading
/content/journal/jmm/10.1099/00222615-40-3-179
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error