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Volume 39, Issue 1

The inflammatory response of the gastric mucosa of mice experimentally infected with Free

Abstract

Summary

To establish an experimental model to study gastric spiral non-cultivable bacteria, 30 4-week-old female CFW (LOB) mice were inoculated with porcine gastric mucus containing and 25 mice were inoculated with mucus without . Mice were examined 3, 7, 14, 21, 28 and 60 days after inoculation. Fragments from the membranous, oxyntic and antral gastric mucosa and from the duodenal mucosa were obtained for histological and microbiological analysis. Tightly spiralled bacteria were seen in smears and in histological sections of the antral and oxyntic mucosa from all -infected mice. The pre-formed urease test also gave positive results in both tissues. In control mice, no tightly spiralled bacteria were seen. By 7 days after inoculation, the test animals had developed an inflammatory infiltrate of mononuclear cells, some neutrophils and a few eosinophils, mainly in the lower third of the antral and oxyntic mucosa, which persisted for the remainder of the observation period. This model can assist in the understanding of several clinical, pathological and immunological aspects of infection with spiral gastric bacteria, particularly those associated with non-cultivable spiral bacteria.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1993
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1993-07-01
2024-09-11
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References

  1. Graham DY. Campylobacter pylori and peptic ulcer disease. Gastroenterology 1989; 96:615–625
     [Google Scholar]
  2. Dent JC, McNulty CAM, Uff JC, Wilkinson SP, Gear MWL. Spiral organisms in the gastric antrum. Lancet 1987; 2:96
     [Google Scholar]
  3. McNulty CAM, Dent JC, Curry A et al. New spiral bacterium in gastric mucosa. J Clin Pathol 1989; 42:585–591
     [Google Scholar]
  4. Solnick JV, O’Rourke J, Lee A, Tompkins LS. Gastrospirillum is a new species of Helicobacter in humans (Abstract). Gastroenterology 1992; 102:Suppl:A699
     [Google Scholar]
  5. Lee A. Spiral microorganisms: What are they? A microbiologic introduction to Helicobacter pylori. Scand J Gastroenterol 1991; 26:Suppl. 187922
     [Google Scholar]
  6. Newell DG, Hudson MJ, Baskerville A. Naturally occurring gastritis associated with Campylobacter pylori infection in the rhesus monkey. Lancet 1987; 2:1338
     [Google Scholar]
  7. Krakowka S, Morgan DM, Kraft WG, Leunk RD. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect Immun 1987; 55:2789–2796
     [Google Scholar]
  8. Jorgensen A, Egnstrand L, Soderlind O, Gustafsson S. Pig as a model for Campylobacter pylori infection. Abstracts of the 10th Congress International Pig Veterinary Society 1988131
     [Google Scholar]
  9. Radin MJ, Eaton KA, Krakowka S et al. Helicobacter pylori gastric infection in gnotobiotic beagle dogs. Infect Immun 1990; 58:2606–2612
     [Google Scholar]
  10. Fox JG, Lee A, Otto G, Taylor NS, Murphy JC. Helicobacter felis gastritis in gnotobiotic rats: an animal model of Helicobacter pylori gastritis. Infect Immun 1991; 59:785–791
     [Google Scholar]
  11. Lee A, Fox JG, Otto G, Murphy J. A small animal model of human Helicobacter pylori active chronic gastritis. Gastroenterology 1990; 99:1315–1323
     [Google Scholar]
  12. Queiroz DMM, Contigli C, Coimbra RS et al. Spiral bacterium associated with gastric, ileal and caecal mucosa of mice. Lab Anim 1992; 26:288–294
     [Google Scholar]
  13. Phillips MW, Lee A. Isolation and characterization of a spiral bacterium from the crypts of rodent gastrointestinal tracts. Appl Environ Microbiol 1983; 45:675–683
     [Google Scholar]
  14. Queiroz DMM, Rocha GA, Mendes EN, Lage AP, Carvalho ACT, Barbosa AJA. Spiral microorganism in stomach of pigs. Vet Microbiol 1990; 24:199–204
     [Google Scholar]
  15. Mendes EN, Queiroz DMM, Rocha GA et al. Histopathological study of porcine gastric mucosa with and without a spiral bacterium (Gastrospirillum suis). J Med Microbiol 1991; 35:345–348
     [Google Scholar]
  16. Rocha GA, Queiroz DMM, Mendes EN, Lage AP, Barbosa AJA. Simple carbol fuchsin staining for showing C. pylori and other spiral bacteria in gastric mucosa. J Clin Pathol 1989; 42:1004–1005
     [Google Scholar]
  17. Queiroz DMM, Mendes EN, Rocha GA. Indicator medium for isolation of Campylobacter pylori. J Clin Microbiol 1987; 25:2378–2379
     [Google Scholar]
  18. Skirrow MB. Campylobacter enteritis: a “new” disease. BMJ 1977; 2:9–11
     [Google Scholar]
  19. Salomon H. Ueber das Spirillum des saugetiermagens undersein verhalten zu den belegzellen. Zentralbl Bakteriol Parasiterkd Infektionskr 1896; 19:433–442
     [Google Scholar]
  20. Kasai K, Kobayashi R. The stomach spirochaete occurring in mammals. J Parasitol 1919; 6:1–11
     [Google Scholar]
  21. Weber AF, Hasa O, Sutter JH. Some observations concerning the presence of spirilla in the fundic glands of dogs and cats. Am J Vet Res 1958; 19:677–680
     [Google Scholar]
  22. Dick E, Lee A, Watson G, O’Rourke J. Use of the mouse for the isolation and investigation of stomach-associated, spiral-helical shaped bacteria from man and other animals. J Med Microbiol 1989; 29:55–62
     [Google Scholar]
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The inflammatory response of the gastric mucosa of mice experimentally infected with “Gastrospirillum suis”
J. Med. Microbiol. 39, 64 (1993); https://doi.org/10.1099/00222615-39-1-64
/content/journal/jmm/10.1099/00222615-39-1-64
/content/journal/jmm/10.1099/00222615-39-1-64
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