1887

Abstract

Summary

Genomic DNA from 48 isolates was digested with low-frequency cleavage (LFC) endonucleases (I and I) and analysed by contour-clamped homogeneous electric fields electrophoresis (CHEF). The restriction patterns generated were reproducible, stable, easy to read and offer a more practical approach than restriction endonuclease analysis (REA) with high-frequency cleavage (HFC) endonucleases. Strains sharing common auxotypes and serovars could be differentiated and correlation with auxotype/serovar (A/S) classes was demonstrated for some, but not all strains. The strains were distributed into 18 A/S classes and 38 and 40 restriction patterns. This greater discriminatory power of CHEF REA should allow subdivision of strains within common A/S classes.

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/content/journal/jmm/10.1099/00222615-38-5-366
1993-05-01
2019-12-08
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-5-366
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