1887

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Volume 38, Issue 5

Pathological Changes in the Rabbit Ileal Loop Model Caused by from Human Colitis Free

Abstract

Summary

Four strains of isolated from children with inflammatory diarrhoea were assayed in the rabbit ileal loop model of infectious diarrhoea. All caused inflammatory reactions with severe macroscopic and microscopic damage in infected rabbit ileal tissue similar to that observed in the patients by endoscopy and histological analysis of colonic biopsies. Haemoglobin and other proteins were observed in loop fluids, consistent with leakage of serum from damaged mucosa. Loop fluids also contained significant bicarbonate concentrations, indicative of an active secretory component similar to that in control loops inoculated with cholera toxin. However, although three of the four clinical strains produced small amounts of a protein immunologically related to cholera toxin none such was detected in either tissues or fluids of infected ileal loops. We propose instead that host-derived mediators of secretion may be important in pathogenesis. A mutant strain of with impaired motility, obtained from the National Collection of Type Cultures, did not induce tissue damage or fluid secretion in rabbit ileal loops.

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© Society for General Microbiology, 1993
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1993-05-01
2024-05-04
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References

  1. Skirrow MB. Campylobacter enteritis: a “new” disease. BMJ 1977; 2:9–11
     [Google Scholar]
  2. Butzler JP, Skirrow MB. Campylobacter enteritis. Clin Gastro enterol 1979; 8:737–765
     [Google Scholar]
  3. Fox GJ. Campylobacteriosis—a new disease in laboratory animals. Lab Anim Sci 1982; 32:625–637
     [Google Scholar]
  4. Van Spreeuwel JP, Duursma GC, Meijer CJLM, Bax R, Rosekrans PCM, Lindeman J. Campylobacter colitis: histological immunohistochemical and ultrastructural findings. Gut 1985; 26:945–951
     [Google Scholar]
  5. Everest PH, Goossens H, Butzler JP et al. Differentiated Caco-2 cells as a model for enteric invasion by Campylobacter jejuni and C. coli. J Med Microbiol 1992; 37:319–325
     [Google Scholar]
  6. Blaser MJ, Perez-Perez G, Smith PF et al. Extraintestinal Campylobacter jejuni and Campylobacter coli infections: host factors and strain characteristics. J Infect Dis 1986; 153:552–559
     [Google Scholar]
  7. Prescott JF, Barker IK, Manninen KI, Miniats OP. Campylo bacter jejuni colitis in gnotobiotic dogs. Can J Comp Med 1981; 45:377–383
     [Google Scholar]
  8. Fitzgeorge RB, Baskerville A, Lander KP. Experimental in fection of Rhesus monkeys with a human strain of Campylobacter jejuni . J Hyg 1981; 86:343–351
     [Google Scholar]
  9. Russell RG, Blaser MJ, Sarmiento JI, Fox J. Experimental Campylobacter jejuni infection in Macaca nemestrina. Infect Immun 1989; 57:1438–1444
     [Google Scholar]
  10. Field LH, Underwood JL, Berry LJ. The role of gut flora and animal passage in the colonization of adult mice with Campylobacter jejuni . J Med Microbiol 1984; 17:59–66
     [Google Scholar]
  11. Blaser MJ, Duncan DJ, Smith PF. Pathogenesis of campylo bacter infection: clearance of bacteraemia in mice. Micro-ecol Ther 1984; 14:103–108
     [Google Scholar]
  12. Kazmi SU, Robertson BS, Stem NJ. Animal passed, virulence enhanced Campylobacter jejuni causes enteritis in neonatal mice. Curr Microbiol 1984; 11:159–164
     [Google Scholar]
  13. Humphrey CD, Montag DM, Pittman FE. Experimental infection of hamsters with Campylobacter jejuni . J Infect Dis 1985; 151:485–493
     [Google Scholar]
  14. Spira WM, Sack RB, Froehlich JL. Simple adult rabbit model for Vibrio cholerae and enterotoxigenic Escherichia coli diarrhea. Infect Immun 1981; 32:739–747
     [Google Scholar]
  15. Spira WM, Sack RB. Kinetics of early cholera infection in the removable intestinal tie-adult rabbit diarrhea model. Infect Immun 1982; 35:952–957
     [Google Scholar]
  16. Caldwell MB, Walker RI, Stewart SD, Rogers JE. Simple adult rabbit model for Campylobacter jejuni enteritis. Infect Immun 1983; 42:1176–1182
     [Google Scholar]
  17. Williams Smith H, Halls S. Observations by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits. J Pathol Bacteriol 1967; 93:499–530
     [Google Scholar]
  18. Sedlock DM, Deibel RH. Detection of salmonella enterotoxin using rabbit ileal loops. Can J Microbiol 1978; 24:268–273
     [Google Scholar]
  19. Mitchell TJ, Ketley JM, Haslam SC et al. Effect of toxin A and B of Clostridium difficile on rabbit ileum and colon. Gut 1986; 27:78–85
     [Google Scholar]
  20. Leitch GJ. Cholera enterotoxin-induced mucus secretion and increase in the mucus blanket of the rabbit ileum in vivo . Infect Immun 1988; 56:2871–2875
     [Google Scholar]
  21. Wallis TS, Hawker RJH, Candy DCA et al. Quantification of the leucocyte influx into rabbit ileal loops induced by strains of Salmonella typhimurium of different virulence. J Med Microbiol 1989; 30:149–156
     [Google Scholar]
  22. McCardell BA, Madden JM, Bier JW, Lee EC, Dallas HL. In Pearson AD, Skirrow MB, Rowe B, Davies JR, Jones DM. eds Campylobacter II. London: Public Health Laboratory Service; 1983:128
     [Google Scholar]
  23. Svennerholm AM, Holmgren J. Identification of heat-labile enterotoxin by means of a ganglioside immunosorbent assay (GM1 -ELISA) procedure. Curr Microbiol 1978; 1:19–23
     [Google Scholar]
  24. Abimiku AG, Dolby JM, Borriello SP, Davies H. Protection against gastrointestinal colonization by Campylobacter jejuni in the infant mouse model. Microecol Ther 1989; 18:233–239
     [Google Scholar]
  25. Bradford MM. A rapid and sensitive method for the quanti tation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248–254
     [Google Scholar]
  26. Miles AA, Misra SS, Irwin JO. The estimation of the bac tericidal power of the blood. J Hyg Camb 1938; 38:732–749
     [Google Scholar]
  27. Taylor PW. Measurement of the bactericidal activity of serum. In Sussman M. ed The virulence of Escherichia coli . London: Academic Press; 1985:445–456
     [Google Scholar]
  28. Skirrow MB, Jones DM, Sutcliffe E, Benjamin J. Campylo bacter bacteraemia in England and Wales: an update. Microb Ecol Health Dis 1991; 4:S3
     [Google Scholar]
  29. Blaser MJ, Smith PF, Kohler PF. Susceptibility of Campylo- bacter isolates to the bactericidal activity of human serum. J Infect Dis 1985; 151:227–235
     [Google Scholar]
  30. Ketley JM, Mitchell TJ, Candy DCA, Burdon DW, Stephen J. The effects of Clostridium difficile crude toxins and toxin A on ileal and colonic loops in immune and non-immune rabbits. J Med Microbiol 1987; 24:41–52
     [Google Scholar]
  31. Perez-Perez G, Cohn DL, Guerrant RL, Patton CM, Reller LB, Blaser MJ. Clinical and immunological significance of cholera-like toxin and cytotoxin production by Campylo-bacter species in patients with acute inflammatory diarrhea in the USA. J Infect Dis 1989; 160:460–468
     [Google Scholar]
  32. Field M. Secretion of electrolytes and water by mammalian small intestine.. In Johnson LR. ed Physiology of the gastrointestinal tract, vol 2. New York: Raven Press; 1981963–982
     [Google Scholar]
  33. Mathan VI, Rajan DP, Klipstein FA, Engert RF. Entero toxigenic Campylobacter jejuni among children in South India. Lancet 1984; 2:981
     [Google Scholar]
  34. Black RE, Levine MM, Clements ML, Hughes TP, Blaser MJ. Experimental Campylobacter jejuni infection in humans. J Infect Dis 1988; 157:472–479
     [Google Scholar]
  35. Gots RE, Formal SB, Giannella RA. Indomethacin inhibition of Salmonella typhimurium, Shigella flexneri, and cholera- mediated rabbit ileal secretion. J Infect Dis 1974; 130:280–284
     [Google Scholar]
  36. Giannella RA. Importance of the intestinal inflammatory reaction in salmonella-mediated intestinal secretion. Infect Immun 1979; 23:140–145
     [Google Scholar]
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Pathological Changes in the Rabbit Ileal Loop Model Caused by Campylobacter Jejuni from Human Colitis
J. Med. Microbiol. 38, 316 (1993); https://doi.org/10.1099/00222615-38-5-316
/content/journal/jmm/10.1099/00222615-38-5-316
/content/journal/jmm/10.1099/00222615-38-5-316
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