1887

Abstract

Summary

sp., grown in tryptone soya broth supplemented with yeast extract, 0.6%, pH 7.5, and incubated with agitation at 100 oscillations/min for 15 h at 37°C produced optimal amounts of β-haemolysin and cytotonic enterotoxin. More prolonged incubation resulted in the loss of enterotoxic activity and anion exchange chromatographic analysis indicated the presence of a moiety capable of brerking down the toxin. Anion exchange fast protein liquid chromatography resulted in a single peak of haemolytic activity and two peaks with enterotoxic activity. The cytotonic enterotoxin was purified from the fraction most active in the infant mouse assay; the second peak, which did not cross-react immunologically, may represent a second cytotonic enterotoxi. Neither peak was observed in the chromatographic fractions of filtrates from strains devoid of activity in the infant mouse assay. Purified enterotoxin, estimated to have a mol. wt of 15kDa by SDS-PAGE, caused fluid accumulation in the infant mouse assay, was non-haemolytic to rabbit erythrocytes, caused an increase in cAMP activity in tissue culture cells and did not cross-react immunologically with components of cholera toxin or the whole toxin. Purified β-haemolysin had an estimated mol. wt of 55 kDa, lysed rabbit erythrocytes and did not cause fluid accumulation in the infant mouse test.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-3-227
1993-03-01
2023-01-31
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/3/medmicro-38-3-227.html?itemId=/content/journal/jmm/10.1099/00222615-38-3-227&mimeType=html&fmt=ahah

References

  1. Lautrop H. Aeromonas hydrophila isolated from human faeces and its possible pathological significance. Acta Pathol Microbiol Scand 1961; 144:299–301
    [Google Scholar]
  2. Cumberbatch N, Gurwith MJ, Langston C, Sack RB, Brunton JL. Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrhoeal disease. Infect Immun 1979; 23:829–837
    [Google Scholar]
  3. Gracey M, Burke V, Robinson J. Aeromonas-associated gas troenteritis. Lancet 1982; 2:1304–1306
    [Google Scholar]
  4. Agger WA, McCormick JD, Gurwith MJ. Clinical and micro biological features of Aeromonas hydrophila-associated diarrhoea. J Clin Microbiol 1985; 21:909–913
    [Google Scholar]
  5. Burke V, Gracey M, Robinson J, Peck D, Beaman J, Bundell C. The microbiology of childhood gastroenteritis: Aeromonas species and other infectious agents. J Infect Dis 1983; 148:68–74
    [Google Scholar]
  6. Burke V, Robinson J, Berry RJ, Gracey M. Detection of enterotoxins of Aeromonas hydrophila by a suckling mouse test. J Med Microbiol 1981; 14:401–408
    [Google Scholar]
  7. Ljungh A. Aeromonas-toxins and other virulence factors. Proceedings of the 1st International Workshop on Aeromonas and Plesiomonas, Manchester; 198633–35
    [Google Scholar]
  8. Morgan DR, Johnson PC, DuPont HL, Satterwhite TK, Wood LV. Lack of correlation between known virulence properties of Aeromonas hydrophila and enteropathogenicity for humans. Infect Immun 1985; 50:62–65
    [Google Scholar]
  9. Turnbull PCB, Lee JV, Miliotis MD. et al. Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J Clin Microbiol 1984; 2:175–180
    [Google Scholar]
  10. Elliott RP, Clark DS, Lewis KH, Lundbeck H, Olson JC, Simonsen B. (eds) Enumeration of mesophilic aerobes. In: Micro-organisms in foods. University of Toronto Press; ICMSF: 1978119–120
    [Google Scholar]
  11. Laemmli UK. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  12. Ljungh A, Eneroth P, Wadstrom T. Cytotonic enterotoxin from Aeromonas hydrophila. Toxicon 1982; 20:787–794
    [Google Scholar]
  13. Rose JM, Houston CW, Coppenhaver DH, Dixon JD, Kurosky A. Purification and chemical characterisation of a cholera toxin-cross-reactive cytolytic enterotoxin purified from a human isolate of Aeromonas hydrophila. Infect Immun 1989; 57:1165–1169
    [Google Scholar]
  14. Asao T, Kinoshita Y, Kosaki S, Uemura T, Sakaguchi G. Purification and some properties of Aeromonas hydrophila hemolysin. Infect Immun 1984; 46:122–127
    [Google Scholar]
  15. Potomski J, Burke V, Watson I, Gracey M. Purification of cytotoxic enterotoxin of Aeromonas sobria by use of monoclonal antibodies. J Med Microbiol 1987; 23:171–177
    [Google Scholar]
  16. Chakraborty T, Montenegro MA, Sanyal SC, Helmuth R, Bulling E, Timmis KN. Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin. Infect Immun 1984; 46:435–441
    [Google Scholar]
  17. Potomski J, Burke V, Robinson J, Fumarola D, Miragliotta G. Aeromonas cytotonic enterotoxin cross-reactive with cholera toxin. J Med Microbiol 1987; 23:179–186
    [Google Scholar]
  18. Chopra AK, Houston CW, Kurosky A. Genetic variation in related cytolytic toxins produced by different species of Aeromonas. FEMS Microbiol Lett 1991; 62:231–237
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-3-227
Loading
/content/journal/jmm/10.1099/00222615-38-3-227
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error