1887

Journal of Medical Microbiology logo

Volume 37, Issue 2

Characterisation of non-pigmented species of the genus by polyacrylamide gel electrophoresis Free

Abstract

Summary

Gram-negative anaerobic bacilli previously known as the melaninogenicus-oralis group of have been assigned to a new genus. The non-pigmented members of this genus share several general characteristics and cannot be readily distinguished by routine tests. A polyacrylamide slab gel electrophoresis procedure, with visual analysis of protein patterns, was used to compare cellular protein patterns from clinical isolates with those from collection (reference) strains. Reference strains of (.) and , and 91 non-pigmented isolates from patients with adult periodontal disease were examined by conventional biochemical tests, gas-liquid chromatography (GLC) and enzyme tests, and whole-cell protein profiles were obtained by SDS-PAGE. There was close correlation between patterns of results in biochemical and GLC tests and the SDS-PAGE profiles, and the species were readily distinguished in SDS-PAGE. The periodontal isolates were assigned to 10 groups by conventional test reaction patterns and nine groups by SDS-PAGE; the profiles of 79 isolates corresponded to those of seven species reference strains. By SDS-PAGE, clinical isolates of (42 isolates) and (eight isolates) showed good similarity with reference strains. However, for (15), (7), (4), (2) and (1), clinical isolates showed some minor variations from reference strains. Twelve isolates remained undesignated in SDS-PAGE analysis. Variant SDS-PAGE profiles divided clinical isolates of into two subgroups and those of into five subgroups.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1992
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-2-83
1992-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/2/medmicro-37-2-83.html?itemId=/content/journal/jmm/10.1099/00222615-37-2-83&mimeType=html&fmt=ahah

References

  1. Duerden B. I. The isolation and identification of Bacteroides spp. from the normal human gingival flora. J Med Microbiol 1980; 13:89–101
     [Google Scholar]
  2. Shah H. N., Collins M. D. Prevotella, a new genus to include Bacteroides melatiinogenicus and related species formerly classified in the genus Bacteroides . Int J Syst Bacteriol 1990; 40:205–208
     [Google Scholar]
  3. Duerden B. I., Goodwin L., O’neil T. C. A. Identification of Bacteroides species from adult periodontal disease. J Med Microbiol 1987; 24:133–137
     [Google Scholar]
  4. Haapasalo M. The genus Bacteroides in human dental root canal infections. Taxonomic, ultrastructural and clinical studies. Proc Finn Dent Soc 1986; 82: Suppl XI-XIII1–87
     [Google Scholar]
  5. Holdeman L. V., Johnson J. L. Bacteroides disiens sp. nov. and Bacteroides bivius sp. nov. from human clinical infections. Int J Syst Bacteriol 1977; 27:337–45
     [Google Scholar]
  6. Brusis T., Luckhaupt H. Anaerobier infektionen bei exulz-erierten Tumoren im Kopf-Hals-Bereich. Ein Beitrag zum Foetorproblem. Laryngol Rhinol Otol 1986; 65:65–68
     [Google Scholar]
  7. Brook I. Direct and indirect pathogenicity of anaerobic bacteria in respiratory tract infections in children. Adv Pediatr 1987; 34:357–377
     [Google Scholar]
  8. Shah H. N., Collins M. D. Proposal to restrict the genus Bacteroides (Castellani and Chalmers) to Bacteroides fragilis and closely related species. Int J Syst Bacteriol 1989; 39:85–87
     [Google Scholar]
  9. Storm A., Dyer J. K., Marsh C., Tribble J. K. Identification and characterisation of species of the family Bacteroidaceae by polyacrylamide gel electrophoresis. J Dent Res 1976; 55:252–256
     [Google Scholar]
  10. Moore W. E. C., Hash D. E., Holdeman L. V., Cato E. P. Poly acrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl Environ Microbiol 1980; 39:900–907
     [Google Scholar]
  11. Holbrook W. P., Ogston S. A., Ross P. W. A method for the isolation of Bacteroides melaninogenicus from the human mouth. J Med Microbiol 1978; 11:203–207
     [Google Scholar]
  12. Yousefi-Mashouf R., Duerden B. I. An identification scheme for oral non-pigmented Prevotella (Bacteroides) species. Microbial Ecol Health Dis (in press)
    [Google Scholar]
  13. Rotimi V. O., Faulkner J., Duerden B. I. Rapid methods for identification of clinical isolates of gram-negative anaerobic bacilli. Med Lab Sci 1980; 37:331–339
     [Google Scholar]
  14. Casals J. B., Pringle N. Diagnostic tablets for bacterial identification. Rosco Diagnostica, Taastrop, Denmark 1987
     [Google Scholar]
  15. Popovic-Uroic T., Patton C. M., Nicholson M. A., Kiehlbauch J. A. Evaluation of the indoxyl acetate hydrolysis test for rapid differentiation of Campylobacter, Helicobacter, and Wolinella species. J Clin Microbiol 1990; 28:2335–2339
     [Google Scholar]
  16. Holdeman L. V., Cato E. P., Moore W. E. C. (eds) Anaerobe laboratory manual 4th edn Blacksburg V. A.: Anaerobe Laboratory, Virginia Polytechnic Institute and State University; 1977
     [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
     [Google Scholar]
  18. Watabe J., Benno Y., Mitsuoka T. Taxonomic study of Bacteroides oralis and related organisms and proposal of Bacteroides veroralis sp. nov. Int J Syst Bacteriol 1985; 33:57–64
     [Google Scholar]
  19. Shah H. N., Collins M. D., Watabe J., Mitsuoka T. Bacteroides oulorum sp. nov., a nonpigmented saccharolytic species from the oral cavity. Int J Syst Bacteriol 1985; 35:193–197
     [Google Scholar]
  20. Shah H. N., Collins M. D. Bacteroides buccalis, sp. nov., Bacteroides denticola, sp. nov., Bacteroides pentosaceus, sp. nov., new species of the genus Bacteroides from the oral cavity. Zentralbl Bakteriol Hyg Abt I Orig C 1981; 2:235–241
     [Google Scholar]
  21. Cato E. P., Kelley R. W., Moore W. E. C., Holdeman L. V. Bacteroides zoogleof ormans (Weinberg, Nativelle, and Prevot 1983) corrig., comb, nov.: emended description. Int J Syst Bacteriol 1982; 32:271–274
     [Google Scholar]
  22. Haapasalo M. Bacteroides buccae and related taxa innecrotic root canal infections. J Clin Microbiol 1986; 24:940–944
     [Google Scholar]
  23. Johnston B. L., Edelstein M. A. C., Holloway E. Y., Finegold S. M. Bacteriologic and clinical study of Bacteroides oris and Bacteroides buccae . J Clin Microbiol 1987; 25:491–493
     [Google Scholar]
  24. Johnson J. L., Holdeman L. V. Bacteroides capillus Komman and Holt and Bacteroides pentosaceus Shah and Collins, later synonyms of Bacteroides buccae Holdeman. Int J Syst Bacteriol 1985; 35:114
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-2-83
Loading
Characterisation of non-pigmented species of the genus Prevotellaby polyacrylamide gel electrophoresis
J. Med. Microbiol. 37, 83 (1992); https://doi.org/10.1099/00222615-37-2-83
/content/journal/jmm/10.1099/00222615-37-2-83
/content/journal/jmm/10.1099/00222615-37-2-83
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error