1887

Abstract

Summary

Transesterification of bacterial fatty acids to methyl esters with trimethylsulphonium hydroxide (TMSH) was compared with a conventional method for the identification of species by capillary gas chromatography. There was an extensive coincidence in the gas chromatographic profiles of bacterial fatty acid methyl esters (FAMEs) obtained by the two methods. However, the TMSH procedure needs less initial material and is much more simple and rapid. The chromatographic pattern of FAMEs obtained from a single colony is sufficient for the identification of the genus , and can be clearly distinguished from other species.

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/content/journal/jmm/10.1099/00222615-36-6-371
1992-06-01
2020-01-22
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-36-6-371
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