1887

Abstract

Summary

Transesterification of bacterial fatty acids to methyl esters with trimethylsulphonium hydroxide (TMSH) was compared with a conventional method for the identification of species by capillary gas chromatography. There was an extensive coincidence in the gas chromatographic profiles of bacterial fatty acid methyl esters (FAMEs) obtained by the two methods. However, the TMSH procedure needs less initial material and is much more simple and rapid. The chromatographic pattern of FAMEs obtained from a single colony is sufficient for the identification of the genus , and can be clearly distinguished from other species.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-36-6-371
1992-06-01
2024-10-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/36/6/medmicro-36-6-371.html?itemId=/content/journal/jmm/10.1099/00222615-36-6-371&mimeType=html&fmt=ahah

References

  1. Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. Cellular fatty acid composition of isolates from Legionnaires’ disease. J Clin Microbiol 1977; 6:140–143
    [Google Scholar]
  2. Moss C. W. Gas-liquid chromatography as an analytical tool in microbiology. J Chromatogr 1981; 203:337–347
    [Google Scholar]
  3. Lambert M. A., Moss C. W. Cellular fatty acids compositions and isoprenoid quinone contents of 23 Legionella species. J Clin Microbiol 1989; 27:465–473
    [Google Scholar]
  4. Wait R., Dennis P. J., Hudson M. J. The use of gas chromatogra-phy/mass spectrometry for the identification and taxonomy of Legionella species. Biochem Soc Trans (London) 1986; 14:965–966
    [Google Scholar]
  5. Moss C. W., Bibb W. F., Karr D. E., Guerrant G. O. Chemical analysis of the genus Legionella: fatty acids and isoprenoid quinones. INSERM Colloq 1983; 114:375–381
    [Google Scholar]
  6. Miller L., Berger T. Bacteria identification by gas chromatogram phy of whole cell fatty acids. Hewlett-Packard Application Note 228–41; 19851–8
  7. Yamauchi K., Tanabe T., Kinoshita M. Trimethylsulfonium hydroxide: a new methylating agent. J Org Chem 1979; 44:638–639
    [Google Scholar]
  8. Butte W. Rapid method for the determination of fatty acid profiles from fats and oils using trimethylsulphonium hydroxide for transesterification. J Chromatogr 1983; 261:142–145
    [Google Scholar]
  9. Matter L., Schenker D., Husmann H., Schomburg G. Characterization of animal fats via the GC pattern of fame mixtures obtained by transesterification of the triglycerides. Chromatographia 1989; 27:31–36
    [Google Scholar]
  10. Schulte E., Weber K. Schnelle Herstellung der Fettsäuremethylester aus Fetten mit Trimethylsulfoniumhydroxid oder Natriummethylat. Fat Sci Technol 1989; 91:181–183
    [Google Scholar]
  11. Muller K.-D., Husmann H., Nalik H. P. A new and rapid method for the assay of bacterial fatty acids using high resolution capillary gas chromatography and trimethylsulfonium hydroxide. Zentralbl Bakteriol 1990; 274:174–182
    [Google Scholar]
  12. Muller K.-D., Husmann H., Nalik H. P., Schomburg G. Trans esterification of fatty acids from micro-organisms and human blood serum by trimethylsulfonium hydroxide (TMSH) for GC analysis. Chromatographia 1990; 30:245–248
    [Google Scholar]
  13. Edelstein P. H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol 1981; 14:298–303
    [Google Scholar]
  14. Edelstein P. H. Comparative study of selective media for isolation of Legionella pneumophila from potable water. J Clin Microbiol 1982; 16:697–699
    [Google Scholar]
  15. Wadowsky R. M., Yee R. B. Glycine-containing selective medium for isolation of Legionellaceae from environmental specimens. Appl Environm Microbiol 1981; 42:768–772
    [Google Scholar]
  16. Thomann W. R., Hill G. B. Modified extraction procedure for gas-liquid chromatography applied to the identification of anaerobic bacteria. J Clin Microbiol 1986; 23:392–394
    [Google Scholar]
  17. Wilkinson H. W. Legionellosis. In Balows A., Hausler W. J., Ohashi M., Turano A. (eds) Laboratory diagnosis of infectious diseases vol I New York: Springer; 1988320–332
    [Google Scholar]
  18. Weber L. Comparison of different sample preparation methods for bacteria. Identification by capillary gas chromatography. J High Resolut Chromatogr Commun 1990; 13:851–854
    [Google Scholar]
  19. Drucker D. B. Gas-liquid chromatographic chemotaxonomy. In Norris J. R. (ed) Methods in microbiology vol 9 London: Academic Press; 197651–125
    [Google Scholar]
  20. Miller L. T. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586
    [Google Scholar]
  21. Anon Esterification and acylation. GC Bulletin 721 J Belle-fonte, Supelco Inc; 19797
    [Google Scholar]
  22. Anon Identifying bacteria by analyzing their cellular fatty acids. GC Bulletin 767E Bellefonte, Supelco Inc; 19862
    [Google Scholar]
  23. Wade T. J., Mandle R. J. New gas chromatographic characterization procedure: preliminary studies of some Pseudomonas species. Appl Microbiol 1974; 27:303–311
    [Google Scholar]
  24. Lambert M. A., Moss C. W. Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria. J Clin Microbiol 1983; 18:1370–1377
    [Google Scholar]
  25. Husmann H., Schomburg G., Muller K.-D., Nalik H. P., von Recklinghausen G. Gas chromatographic FAME analysis for characterization of bacteria: formation of aldehydes from 3-hydroxy fatty acid methyl esters during sample introduction. J High Resolut Chromatogr Commun 1990; 13:780–782
    [Google Scholar]
  26. Ehret W., Jacob K., Ruckdeschel G. Identification of clinical and environmental isolates of Legionella pneumophila by analysis of outer-membrane proteins, ubiquinones and fatty acids. Zentralbl Bakteriol Mikrobiol Hyg A 1987; 266:261–275
    [Google Scholar]
  27. Marmet D., Bomstein N., Fleurette J. Identification des Legionella par analyse des acides gras en chromatographie phase gazeuse (CPG) et des ubiquinones en chromatographie liquide haute performance (CLHP). Ann Biol Clin 1988; 46:371–375
    [Google Scholar]
  28. Moss C. W., Bibb W. F., Karr D. E., Guerrant G. O., Lambert M. A. Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov. J Clin Microbiol 1983; 18:917–919
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-36-6-371
Loading
/content/journal/jmm/10.1099/00222615-36-6-371
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error