1887

Abstract

Summary

A collection of 201 isolates of was examined: 152 methicillin-sensitive (MSSA) comprised 48 blood culture isolates (BC) and 58 isolates from routine diagnostic specimens (RD) from Glasgow Royal Infirmary (GRI), and 46 strains from nasal swabs of patients attending a general practitioner (GP); 49 isolates were of methicillin-resistant (MRSA) from GRI. We have previously shown that the MRSA could be divided into two sub-groups on the basis of sensitivity or resistance to aminoglycoside antibiotics. Production of enterotoxins A, B, C and D, and α-, β-, γ- and δ-haemolysins was detected by reverse passive latex agglutination (RPLA) and agar overlay methods respectively: 60% of BC MSSA and a similar proportion of MSSA from other sources produced enterotoxin; 87% of aminoglycoside-sensitive MRSA produced enterotoxin (89% of these produced enterotoxin A alone) whereas only 27% of aminoglycoside-resistant MRSA were enterotoxin-positive, significantly less than either MSSA or aminoglycoside-sensitive MRSA. The proportion of haemolysin-producing isolates did not differ amongst the isolates of MSSA and MRSA; there was no difference in the distributions of haemolysins between aminoglycoside-sensitive and -resistant strains of MRSA. GP MSSA had higher and lower numbers of γ- and δ-haemolysin producers respectively than other isolates. α-Haemolysin producers were commoner amongst MRSA isolates, which were also more likely than MSSA isolates to produce several haemolysins. Differences in enterotoxin production between aminoglycoside-sensitive and -resistant MRSA isolates reflect subgroups previously defined by biotype, phage type, immunoblot and restriction enzyme fragmentation pattern data, and provide further evidence for the existence of two major MRSA clones in GRI.

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1992-03-01
2024-12-03
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References

  1. Adlam C., Easmon C. S. F. Immunity and hypersensitivity to staphylococcal infection. In Staphylococci and staphylococcal infections vol 1 clinical and epidemiological aspects London: Academic Press; 1983275–323
    [Google Scholar]
  2. Shanson D. C. Staphylococcal infections in hospital. Br J Hosp Med 1986; 35:312–320
    [Google Scholar]
  3. Musher D. M., Verner E. F. Treatment of infections due to Staphylococcus aureus. In Jeljaszewicz J. (ed) The staphylococci Zbl Bakteriol: Mikrobiol Hyg; 1985 Suppl 14407–419
    [Google Scholar]
  4. Waldvogel F. A. Treatment of infections due to methicillin-resistant Staphylococcus aureus. J Hosp Infect 1986; 7: Suppl A 37–46
    [Google Scholar]
  5. Marples R. R., Cooke E. M. Current problems with methicillin-resistant Staphylococcus aureus. J Hosp Infect 1988; 11:381–392
    [Google Scholar]
  6. Pavillard R., Harvey K., Douglas D. Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Med J Aust 1982; 1:451–454
    [Google Scholar]
  7. Turnidge J., Lawson P., Munro R., Benn R. A national survey of antimicrobial resistance in Staphylococcus aureus in Australian teaching hospitals. Med J Aust 1989; 150:69–72
    [Google Scholar]
  8. Locksley R. M., Cohen M. L., Quinn T. C. Multiply antibiotic-resistant Staphylococcus aureus: introduction, transmission and evolution of nosocomial infection. Ann Intern Med 1982; 97:317–324
    [Google Scholar]
  9. Cafferkey M. T., Coleman D. C., McGrath B. Methicillin-resistant Staphylococcus aureus in Dublin 1971–84. Lancet 1985; 2:705–708
    [Google Scholar]
  10. Morgan M. G., Harte-Barry M. J. Methicillin-resistant Staphylococcus aureus: a ten-year survey in a Dublin hospital. J Hosp Infect 1989; 14:357–362
    [Google Scholar]
  11. Marples R. R. Methicillin-resistant Staphylococcus aureus. Curr Opin Infect Dis 1988; 1:722–726
    [Google Scholar]
  12. Casewell M. W. Epidemiology and control of the ‘modem’ methicillin-resistant Staphylococcus aureus. J Hosp Infect 1986; 7: Suppl A 1–11
    [Google Scholar]
  13. Maple P. A. C., Hamilton-Miller J. M. T., Brumfitt W. World-wide antibiotic resistance in methicillin-resistant Staphylococcus aureus. Lancet 1989; 1:537–540
    [Google Scholar]
  14. Lacey R. W. Multi-resistant Staphylococcus aureus—a suitable case for inactivity?. J Hosp Infect 1987; 9:103–105
    [Google Scholar]
  15. Lacey R. W., Barr K. W., Barr V. E., Inglis T. J. Properties of methicillin-resistant Staphylococcus aureus colonizing patients in a burns unit. J Hosp Infect 1986; 7:137–148
    [Google Scholar]
  16. Roberts J. I. S., Gaston M. A. Protein A and coagulase expression in epidemic and non-epidemic Staphylococcus aureus. J Clin Pathol 1987; 40:837–840
    [Google Scholar]
  17. Jordens J. Z., Duckworth G. J., Williams R. J. Production of “virulence factors” by “epidemic” methicillin-resistant Staphylococcus aureus in vitro. J Med Microbiol 1989; 30:245–252
    [Google Scholar]
  18. Cookson B., Talsania H., Naidoo J., Phillips I. Strategies for typing and properties of epidemic methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol 1986; 5:702–709
    [Google Scholar]
  19. Humphreys H., Keane C. T., Hone R. Enterotoxin production by Staphylococcus aureus isolates from cases of septicaemia and from healthy carriers. J Med Microbiol 1989; 28:163–172
    [Google Scholar]
  20. Duckworth G. J., Oppenheim B. A. Enterotoxin production in epidemic methicillin-resistant Staphylococcus aureus. Lancet 1986; 1:565–566
    [Google Scholar]
  21. Coia J. E., Noor-Hussain I., Platt D. J. Plasmid profiles and restriction enzyme fragmentation patterns of plasmids of methicillin-sensitive and methicillin-resistant isolates of Staphylococcus aureus from hospital and the community. J Med Microbiol 1988; 27:271–276
    [Google Scholar]
  22. Coia J. E., Thomson-Carter F., Baird D., Platt D. J. Characterisation of methicillin-resistant Staphylococcus aureus by biotyping, immunoblotting and restriction enzyme fragmentation patterns. J Med Microbiol 1990; 31:125–132
    [Google Scholar]
  23. Chao L.-P., Birkbeck T. H. Assay of staphylococcal δ-haemolysin with fish erythrocytes. J Med Microbiol 1978; 11:303–313
    [Google Scholar]
  24. Siegel S. Non-parametric statistics for the behavioural sciences. London: McGraw-Hill; 1956104–111
    [Google Scholar]
  25. Christensson B., Hedstrom S. A. Biochemical and biological properties of Staphylococcus aureus septicaemia strains in relation to clinical characteristics. Scand J Infect Dis 1986; 18:297–303
    [Google Scholar]
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