1887

Abstract

Summary

Seventy amikacin-resistant clinical isolates of gram-negative bacteria belonging to nine genera were examined by immunoblotting and by DNA-DNA hybridisation for the presence of ACC(6′)1b enzyme, previously called AAC(6′)-4, or its encoding gene The organisms mostly had resistance profiles compatible with AAC(6′) production and were from South and North America, the Far East and Europe. Polyclonal (rabbit) anti-AAC(6′)-1b antisera and an intragenic () probe derived from the multiresistance plasmid pAZ007 were used. The gene was found to be widespread. Positive hybridisation, and immunologically cross-reactive proteins, were observed in 44% of the isolates examined. They were present most frequently (£70%) in isolates of and spp., but less often (£25%) in and spp. The strains that reacted with the probe produced enzymes that varied in their apparent mol. wts between 24000 and 26000. The existence of multiple electrophoretic forms of amikacin-acetylating enzymes of the ACC(6′)-1b type may be useful in epidemiological surveys of AAC(6′)-mediated amikacin resistance.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-36-2-83
1992-02-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/36/2/medmicro-36-2-83.html?itemId=/content/journal/jmm/10.1099/00222615-36-2-83&mimeType=html&fmt=ahah

References

  1. Price K. E., Kresel P. A., Farchione L. A., Siskin S. B., Karpow S. A. Epidemiological studies of aminoglycoside resistance in the U.S.A.. J Antimicrob Chemother 1981; 8: Suppl A 89–105
    [Google Scholar]
  2. Levine J. F., Maslow M. J., Leibowitz R. E. Amikacin-resistant gram-negative bacilli: correlation of occurrence with amikacin use. J Infect Dis 1985; 151:295–300
    [Google Scholar]
  3. Shimizu K., Kumada T., Hsieh W. -C. Comparison of aminoglycoside resistance patterns in Japan, Formosa, and Korea, Chile, and the United States. Antimicrob Agents Chemother 1985; 28:282–288
    [Google Scholar]
  4. Shaw K. J., Cramer C. A. Rizzo Metal., Isolation, characterization, and DNA sequence analysis of an AAC(6′)-II gene from Pseudomonas aeruginosa. Antimicrob Agents Chemother 1989; 33:2052–2062
    [Google Scholar]
  5. Mitsuhashi S., Kawabe H. Aminoglycoside antibiotic resistance in bacteria. In Whelton A., Neu H. C. (eds) The aminoglycosides New York: Marcel Dekker; 198297–122
    [Google Scholar]
  6. Meyer J. F., Nies B. A., Wiedemann B. Amikacin resistance mediated by multiresistance transposon Tn 2424. J Bacterial 1983; 155:755–760
    [Google Scholar]
  7. Tolmasky M. E., Crosa J. H. Tn 1331, a novel multiresistance transposon encoding resistance to amikacin and ampicillin in Klebsiella pneumoniae. Antimicrob Agents Chemother 1987; 31:1955–1960
    [Google Scholar]
  8. Champion H. M., Bennett P. M., Lewis D. A., Reeves D. S. Cloning and characterization of an AAC(6′) gene from Serratia marcescens. J Antimicrob Chemother 1988; 22:587–596
    [Google Scholar]
  9. Tran Van Nhieu G., Collatz E. Primary structure of an aminoglycoside 6′-N-acetyltransferase, AAC(6′)-4, fused in vivo with the signal peptide of the Tn3-encoded β-lactamase. J Bacteriol 1987; 169:5708–5714
    [Google Scholar]
  10. Tenover F. C., Filpula D., Phillips K. L., Plorde J. J. Cloning and sequencing of a gene encoding an aminoglycoside 6′-N-acetyltransferase from an R factor of Citrobacter diversus. J Bacteriol 1988; 170:471–473
    [Google Scholar]
  11. Teran F. J., Suarez J. E., Mendoza M. C. Cloning, sequencing and use as a molecular probe of a gene encoding an aminoglycoside 6′-N-acetyltransferase of broad substrate profile. Antimicrob Agents Chemother 1991; 35:714–719
    [Google Scholar]
  12. Lambert T., Gerbaud G., Courvalin P. Transferable amikacin resistance in Acinetobacter spp. due to a new type of 3′-aminoglycoside phosphotransferase. Antimicrob Agents Chemother 1988; 8:215–231
    [Google Scholar]
  13. Bongaerts G. P. A., Kaptijn G. M. P. Aminoglycoside phosphotransferase-II-mediated amikacin resistance in Escherichia coli. Antimicrob Agents Chemother 1981; 20:344–350
    [Google Scholar]
  14. Perlin M. H., Lerner S. A. High-level amikacin resistance in Escherichia coli due to phosphorylation and impaired aminoglycoside uptake. Antimicrob Agents Chemother 1986; 29:216–224
    [Google Scholar]
  15. Coombe R. G., George A. M. New plasmid-mediated aminoglycoside adenylyltransferase of broad substrate range that adenylylates amikacin. Antimicrob Agents Chemother 1981; 20:75–80
    [Google Scholar]
  16. Tran Van Nhieu G., Goldstein F. W., Pinto M. E., Acar J. F., Collatz E. Transfer of amikacin resistance by closely related plasmids in members of the family Enterobacteriaceae isolated in Chile. Antimicrob Agents Chemother 1986; 29:833–837
    [Google Scholar]
  17. Tran Van Nhieu G., Collatz E. Heterogeneity of 6′-N-acetyltransferases of type 4 conferring resistance to amikacin and related aminoglycosides in members of the family Enterobacteriaceae. Antimicrob Agents Chemother 1988; 32:1289–1291
    [Google Scholar]
  18. Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1982
    [Google Scholar]
  19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  20. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4354
    [Google Scholar]
  21. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 1982; 119:142–147
    [Google Scholar]
  22. Nobuta K., Tolmasky M. E., Crosa L. M., Crosa J. H. Sequencing and expression of the 6′-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae. J Bacteriol 1988; 170:3769–3773
    [Google Scholar]
  23. Shaw K. J., Sabatelli F., Rizzo M. Correlation between aminoglycoside resistance profiles (AGRPs) and DNA hybridization of clinical isolates. 29th Interscience Conference on Antimicrobial Agents and Chemotherapy; Houston: 1989 Abstract No. 674
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-36-2-83
Loading
/content/journal/jmm/10.1099/00222615-36-2-83
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error