1887

Abstract

In this study the ability of strains of serotype 1 to agglutinate mammalian erythrocytes is attributed to the polysaccharide fraction of bacterial-cell lipopolysaccharide (LPS). LPS obtained from a rough, mutant strain of serotype 1 lacking the O-antigen polysaccharide side-chain, did not agglutinate erythrocytes, clearly demonstrating a link between O-antigen polysaccharides and haemagglutinating activity (HA). Strains of serotype 1 adhered well to cultured Henle Intestinal 407 cells, whereas rough strains adhered poorly. Pre-treatment of bacteria with LPS-specific antisera inhibited both HA and binding to cultured human-intestinal cells. The contribution of the polysaccharide side-chain and its associated HA–which appear to facilitate binding to cultured cells–to bacterial attachment to colonocytes and to the pathogenesis of shigellosis needs to be confirmed in animal studies.

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/content/journal/jmm/10.1099/00222615-34-5-259
1991-05-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-34-5-259
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