1887

Abstract

Summary

The generation of smooth aromatic-dependent serotype Bovismorbificans (Group C2, O6, 8) from a smooth wild-type parent strain by transduction with phage P1, and conjugation with serotype Typhimurium carrying F′-8 is described. The smooth aromatic-dependent serotype Bovismorbificans was non-lethal for mice at an oral challenge dose of 2 × 10 cfu (equivalent to 200 LD50 of the parent, wild-type strain). The safety of the auxotrophic mutant was further substantiated by comparing its multiplication kinetics with that of its virulent parent organisms. Mice immunised with live, smooth aromatic-dependent serotype Bovismorbificans by either the oral or intraperitoneal (i.p.) route were protected against oral challenge with virulent serotype Bovismorbificans; the degree of protection was significantly better (p<0.05) at a challenge dose of 100 or 200 LD50 in mice receiving two rather than one vaccination. In contrast, mice immunised with three doses of the formalin-killed virulent, parent organisms by the i.p. route were not protected, in spite of high antibody titres. Only those mice immunised with the live, smooth aromatic-dependent serotype Bovismorbificans i.p. developed significant (p<0.01–0.05) delayed-type hypersensitivity.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-34-1-57
1991-01-01
2022-09-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/34/1/medmicro-34-1-57.html?itemId=/content/journal/jmm/10.1099/00222615-34-1-57&mimeType=html&fmt=ahah

References

  1. Jelinek P.D., Franklin D. A., Iveson J. B. The recovery of salmonella from sheep that died during transportation by ship. Aust Vet J 1982; 58:170–171
    [Google Scholar]
  2. Richards R. B., Norris R. T., Dunlop R. H., McQuade N. C. Causes of death in sheep exported live by sea. Aust Vet J 1989; 66:33–38
    [Google Scholar]
  3. Wray C, Sojka W. J. Reviews of the progress of dairy science: bovine salmonellosis. J Dairy Rs 1977; 44:383–425
    [Google Scholar]
  4. Smeltzer T, Thomas R., Collins G. Salmonellae on posts, handrails and hands in a beef abattoir. Aust Vet J 1980; 56:184–186
    [Google Scholar]
  5. Samuel J. L., Eccles J. A., Francis J. Salmonella in the intestinal tract and associated lymph nodes of sheep and cattle. J Hyg (Camb) 1981; 87:225–232
    [Google Scholar]
  6. Mukkur T. K. S., McDowell G. H., Stocker B. A. D., Lascelles A. K. Protection against experimental salmonellosis in mice and sheep by immunisation with aromatic-dependent Salmonella typhimurium. J Med Microbiol 1987; 24:11–19
    [Google Scholar]
  7. Litchfield J. T., Wilcoxon F. A simplified method of evaluating dose-effect experiments. J Pharmacol Exp Ther 1949; 96:99–113
    [Google Scholar]
  8. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol 1972; 109:129–135
    [Google Scholar]
  9. Omellas E. P., Stocker B. A. D. Relation of lipopolysaccharide character to PI sensitivity in Salmonella typhimurium. Virology 1974; 60:491–502
    [Google Scholar]
  10. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacterial 1980; 143:926–933
    [Google Scholar]
  11. Nnalue N.A., Stocker B. A. D. Tests of the virulence and live-vaccine efficacy of auxotrophic and galE derivatives of Salmonella choleraesuis. Infect Immun 1987; 55:955–962
    [Google Scholar]
  12. Smith B. P., Reina-Guerra M., Hoiseth S. K. Aromatic-dependent Salmonella typhimurium as modified live vaccines for calves. Am J Vet Res 1984; 45:59–66
    [Google Scholar]
  13. Nnalue N. A., Stocker B. A. D. The effects of O-antigen character and enterobacterial common antigen content on the in-vivo persistence of aromatic-dependent Salmonella sp. live-vaccine strains. Microb Pathog 1987; 3:31–44
    [Google Scholar]
  14. Kuo T. T. Genetics of Salmonella typhimurium: rough mutants, transduction by phage ESI8 and suppression of proline auxotrophy. Ph.D. Thesis Stanford University; California: 1969210 pp
    [Google Scholar]
  15. Collins F. M., Mackaness G. B., Blanden R. V. Infection-immunity in experimental salmonellosis. J Exp Med 1966; 124:601–619
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-34-1-57
Loading
/content/journal/jmm/10.1099/00222615-34-1-57
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error