1887

Abstract

Surmmary

Unlike their parent strains, zidovudine-resistant derivatives of KL16 and NCTC 5710 were found to be incapable of incorporating radiolabeled thymidine into their chromosomal DNA. Since incorporation was still prevented in the presence of EDTA, resistance to zidovudine was not associated with a permeability barrier, but appeared to result from the loss of thymidine kinase activity, required for the phosphorylation of zidovudine. , which is intrinsically zidovudine-resistant, was also shown to be incapable of incorporating thymidine into its DNA, but SK360 and E3T, which are also intrinsically zidovudine-resistant, possessed thymidine kinase activity. This suggests that two distinct mechanisms of resistance to zidovudine exist in bacteria. Zidovudine resistance did not appear to confer resistance to other antibacterial agents.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-33-4-235
1990-12-01
2023-02-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/33/4/medmicro-33-4-235.html?itemId=/content/journal/jmm/10.1099/00222615-33-4-235&mimeType=html&fmt=ahah

References

  1. Mitsuya H, Weinhold KIJ, Furman PA. et al. 3′-azido-3′deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotrophic virus type III/lympadenopathy-asso-ciated virus in vitro. Proc Natl Acad Sci USA 1985; 82:7096–7100
    [Google Scholar]
  2. Mitsuya H, Broder S. Strategies for antiviral therapy in AIDS. Nature 1987; 325:773–778
    [Google Scholar]
  3. Elwell LP, Ferone R, Freeman GA. et al. Antibacterial activity and mechanism of action of 3′-azido-3′-deoxythymidine (BW A509U). Antimicrob Agents Chemother 1987; 31:274–280
    [Google Scholar]
  4. Lewin CS, Amyes SGB. Conditions required for the antibacterial activity of zidovudine. EurJ Clin Microbiol Infect Dis 1989; 8:737–741
    [Google Scholar]
  5. Hirsch MS. Azidothymidine. J Infect Dis 1988; 157:427–431
    [Google Scholar]
  6. Lewin CS, Allen R, Amyes SGB. Zidovudine-resistance in Salmonella typhimurium and Escherichia coli. J Antimicrob Chemother 1990; 25:706–708
    [Google Scholar]
  7. Koch AE, Burchall JJ. Reversal of antimicrobial activity of trimethoprim by thymidine in commercially prepared media. ApplMicrobiol 1971; 22:812–817
    [Google Scholar]
  8. Amyes SGB, Smith JT. Trimethoprim action and its analogy with thymine starvation. Antimicrob Agents Chemother 1974; 5:169–178
    [Google Scholar]
  9. Lewin CS, Smith JT. Bactericidal mechanisms of ofloxacin. J Antimicrob Chemother 1988; 22: Suppl C1–8
    [Google Scholar]
  10. Tennent JM, Young H-K, Lyon BR, Amyes SGB, Skurray RA. Trimethoprim-resistance determinants encoding a dihydrofolate reductase in clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci. J Med Microbiol 1988; 26:67–73
    [Google Scholar]
  11. Smith JT. Awakening the slumbering potential of the 4-quinolone antibacterials. Pharm J 1984; 233:299–305
    [Google Scholar]
  12. Schubach WH, Whitmer JD, Davern CL. Genetic control of DNA initiation in Escherichia coli. J Mol Biol 1973; 74:205–221
    [Google Scholar]
  13. Davis BD, Mingioli ES. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol 1950; 60:17–28
    [Google Scholar]
  14. Lewin CS, Paton R, Watt B, Amyes SGB. Isolation of zidovudine resistant Enterobacteriaceae from AIDS patients. FEMS Microbiol Lett 1990; 70:141–144
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-33-4-235
Loading
/content/journal/jmm/10.1099/00222615-33-4-235
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error