1887

Abstract

Summary

Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. These were predominantly the di-carboxylic porphyrins, deuteroporphyrin and mesoporphyrin, and another which was thought to be pemptoporphyrin. This combination matched those reported previously in normal stools. Protoporphyrin IX was shown not to be the most common fluorescent pigment in pus and was never present alone. However, the di-carboxylic porphyrins may be produced by bacterial metabolism of its labile vinyl side-chains. Black-pigmented bacteroides (the melaninogenicus group of spp. and spp.) were isolated from 12 (63%) of the 19 pus samples; these may produce protoporphyrin IX by the demetallation of haem.

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1990-09-01
2024-12-07
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References

  1. Policard A. Etude sur les aspects offerts par des tumeurs experimentales examinees a la lumiere de Wood. CR Soc Biol Paris 1924; 91:1423–1424
    [Google Scholar]
  2. Kammerer H. Uber das durch darmbakterian gebildte porphyrin und de bedetung der porphyprobe fur die buerteilung der darmfaulnis. Dtsch Arch F Klin Med 1924; 145:257–284
    [Google Scholar]
  3. Ronchese F, Walker BS, Young RM. The reddish orange fluorescence of necrotic cancerous surfaces under the Wood light. AMA Arch Dermatol 1954; 69:31–42
    [Google Scholar]
  4. Sharvill D. On the red fluorescence of certain ulcers under Woods light. Trans St Johns Hosp Derm Soc 1955; 34:32–36
    [Google Scholar]
  5. Myers MB, Cherry G, Bornside BB, Bornside GH. Ultra violet red fluorescence of Bacteroides melaninogenicus. Appl Microbiol 1969; 17:760–762
    [Google Scholar]
  6. Willis AT. Anaerobic bacteriology: clinical and laboratory practice. , 3rd edn.. London: Butterworths; 1977
    [Google Scholar]
  7. Finegold SM, George WL. (eds) Anaerobic infections in humans San Diego: Academic Press; 1989120
    [Google Scholar]
  8. Shah HN, Bonnett R, Mateen B, Williams RA. The porphyrin pigmentation of subspecies of Bacteroides melaninogenicus. Biochem J 1979; 180:45–50
    [Google Scholar]
  9. Lim CK, Li FM, Peters TJ. High performance liquid chromatography of porphyrins. J Chromatogr 1988; 429:123–153
    [Google Scholar]
  10. Lockwood WH, Poulos V, Rossi E, Curnow DH. Rapid procedure for fecal porphyrin assay. Clin Chem 1985; 31:1163–1167
    [Google Scholar]
  11. Shah HN, Collins MD. Proposal for reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a new genus, Porphyromonas. Int J Syst Bacterial 1988; 38:128–131
    [Google Scholar]
  12. French JM, England MT, Lines J, Thonger E. Separation of ether-extractable fecal porphyrins by counter-current distribution. Arch Biochem Biophys 1964; 107:404–418
    [Google Scholar]
  13. Jones CW, Hoffman RA. Porphyrin concentration of the hamster (Mesocricedus auradus) Harderian gland: effects of incubation with delta-aminolevulinic acid and various hormones. Int J Biochem 1976; 7:135–139
    [Google Scholar]
  14. Brazier JS. A note on ultra-violet red fluorescence of anaerobic bacteria in vitro. J Appl Bacteriol 1986; 60:121–126
    [Google Scholar]
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