1887

Abstract

Summary

The tracheobronchial secretions from patients with cystic fibrosis often contain high amounts of free proteases. To evaluate whether human leucocyte elastase (HLE) can favour the persistence of bacterial airways infection, we exposed the frog palate mucosa to HLE and then to radiolabelled and followed the sequence of events by scanning electronmicroscopy. In response to HLE there was a marked outpouring of mucus and a desquamation of the epithelium. was shown to adhere to recently secreted granules of mucus and to the exposed submucosal underlying connective tissues. For the eight different bacterial strains studied, a significative adherence to HLE-injured mucosa was observed only in strains that possessed internal haemagglutinating activity. Neither the presence of fimbriae, nor of the mucoid exopolysaccharide, nor of the bacterial surface haemagglutinating activity could be related to adherence of to the injured mucosa. These results support the hypothesis that HLE enhances bacterial infection of the respiratory mucosa both by inducing mucus hypersecretion and by exposing receptors to the microbial adhesins. It is also suggested that internal lectins may be implicated in adherence to host tissues.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-30-4-285
1989-12-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jmm/30/4/medmicro-30-4-285.html?itemId=/content/journal/jmm/10.1099/00222615-30-4-285&mimeType=html&fmt=ahah

References

  1. Baker N. R., Marcus H. 1982; Adherence of clinical isolates of Pseudomonas aeruginosa to hamster tracheal epithelium in vitro. Current Microbiology 7:35–40
    [Google Scholar]
  2. Bodey G. P., Bolivar R., Fainstein V., Jadeja L. 1983; Infections caused by Pseudomonas aeruginosa. Reviews of Infectious Diseases 5:279–313
    [Google Scholar]
  3. Breuer R., Christensen T. G., Niles R. M., Stone P. J., Snider G. L. 1989; Human neutrophil elastase causes glycoconjugate release from the epithelial cell surface of hamster trachea in organ culture. American Review of Respiratory Disease 139:779–782
    [Google Scholar]
  4. Chailley B., Boisvieux-Ulrich E., Sandoz D. 1982; Ciliary membrane events during ciliogenesis of the quail oviduct. Biology of the Cell 46:51–64
    [Google Scholar]
  5. Cohen A. B., Rossi M. 1983; Neutrophils in normal lungs. American Review of Respiratory Disease 127:S3–S9
    [Google Scholar]
  6. Doig P., Smith N. R., Todd T., Irvin R. T. 1987; Characterization of the binding of Pseudomonas aeruginosa alginate to human epithelial cells. Infection and Immunity 55:1517–1522
    [Google Scholar]
  7. Doig P. 1988; Role of pili in adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells. Infection and Immunity 56:1641–1646
    [Google Scholar]
  8. Duguid J. P., Old D. C. 1980; Adhesive properties of Enterobacteriaceae. In Beachey E. H. (ed) Bacterial adherence Chapman and Hall; London: pp 187–217
    [Google Scholar]
  9. Franklin A. L., Todd T., Gurman G., Black D., Mankinen-Irvin P. M., Irvin R. T. 1987; Adherence of Pseudomonas aeruginosa to cilia of human tracheal epithelial cells. Infection and Immunity 55:1523–1525
    [Google Scholar]
  10. Fuerst J. A., Hayward A. C. 1969; Surface appendages similar to fimbriae (pili) on Pseudomonas species. Journal of General Microbiology 58:227–237
    [Google Scholar]
  11. Gallin I. 1984; Neutrophil specific granules: a fuse that ignites the inflammatory response. Clinical Research 67:320–328
    [Google Scholar]
  12. Geddes D. 1984; Progress in research on cystic fibrosis. Thorax 39:721–725
    [Google Scholar]
  13. Gilboa-Garber N. 1972; Purification and properties of hemagglutinin from Pseudomonas aeruginosa and its reaction with human blood cells. Biochimica et Biophysica Acta 273:165–173
    [Google Scholar]
  14. Gilboa-Garber N. 1983; The biological functions of Pseudomonas aeruginosa lectins. In Bog-Hansen T. C., Spengler G. A. (eds) Lectins: biology, biochemistry, clinical biochemistry vol III Walter de Gruyter & Co; Berlin: pp 495–502
    [Google Scholar]
  15. Gilboa-Garber N. 1986; Lectins of Pseudomonas aeruginosa: properties, biological effects and applications. In Mirelman D. (ed) Microbial lectins and agglutinins: properties and biological activities (Environmental and Applied Microbiology) John Wiley & Sons; London: pp 255–269
    [Google Scholar]
  16. Glick J., Garber N., Shohet D. 1987; Surface haemagglutinating activity of Pseudomonas aeruginosa. Microbios 50:69–80
    [Google Scholar]
  17. Goldstein W., Doring G. 1986; Lysosomal enzymes from polymorphonuclear leukocytes and proteinase inhibitors in patients with cystic fibrosis. American Review of Respiratory Disease 134:49–56
    [Google Scholar]
  18. Greenstone M., Cole P. J. 1985; Ciliary function in healthy and disease. British Journal of Diseases of the Chest 79:9–26
    [Google Scholar]
  19. Hata J. S., Fick R. B. 1987; Cellular mechanisms of Pseudomonas aeruginosa adherence to canine tracheal epithelial cell monolayers. American Review of Respiratory Disease 135A:364
    [Google Scholar]
  20. Havemann K., Gramse M. 1984; Physiology and pathophysiology of neutral proteinases of human granulocytes. In Horl W. H., Heidland A. (eds) Proteases: potential role in health and disease. Advances in experimental medicine and biology vol 167 Plenum Press; New York, London: pp 1–20
    [Google Scholar]
  21. Kim K. C., Wasano K., Niles R. M., Schuster J. E., Stone P. J., Brody J. S. 1987; Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Proceedings of the National Academy of Sciences of the USA 84:9304–9308
    [Google Scholar]
  22. Liu P. V. 1979; Toxins of Pseudomonas aeruginosa. In Doggett R. G. (ed) Pseudomonas aeruginosa: Clinical manifestations of infection and current therapy Academic Press Inc; NewYork: pp 63–87
    [Google Scholar]
  23. Lundberg C., Lonnroth J., Nord C. E. 1982; Adherence in the colonization of Streptococcus pneumoniae in the nasopharynx in children. Infection 10:63–66
    [Google Scholar]
  24. Marcus H., Baker N. R. 1985; Quantification of adherence of mucoid and non-mucoid Pseudomonas aeruginosa to hamster tracheal epithelium. Infection and Immunity 47:723–729
    [Google Scholar]
  25. Marcus H., Austria A., Baker N. R. 1989; Adherence of Pseudomonas aeruginosa to tracheal epithelium. Infection and Immunity 57:1050–1053
    [Google Scholar]
  26. Martodam R. R., Baugh R. J., Twumasi D. Y., Liener I. E. 1979; A rapid procedure for the large scale purification of elastase and cathepsin G from human sputum. Preparative Biochemistry 9:15–31
    [Google Scholar]
  27. McDonald J. A., Baum B. J., Rosenberg D. M., Kelman J. A., Brin S. C., Crystal R. G. 1979; Destruction of a major extracellular adhesive glycoprotein (fibronectin) of human fibroblasts by neutral proteases from polymorphonuclear leukocyte granules. Laboratory Investigation 40:350–357
    [Google Scholar]
  28. Mirelman D., Ofek I. 1986; Introduction to microbial lectins and agglutinins. In Mirelman D. (ed) Microbial lectins and agglutinins: properties and biological activities (Environmental and Applied Microbiology) John Wiley & Sons; London: pp 1–19
    [Google Scholar]
  29. Niederman M. S., Rafferty T. D., Sasaki C. T., Merrill W. W., Matthay R. A., Reynolds H. Y. 1983; Comparison of bacterial adherence to ciliated and squamous epithelial cells obtained from the human respiratory tract. American Review of Respiratory Disease 127:85–90
    [Google Scholar]
  30. Ohlsson K., Olsson I. 1977; The extracellular release of granulocyte collagenase and elastase during phagocytosis and inflammatory processes. Scandinavian Journal of Haematology 19:145–152
    [Google Scholar]
  31. Paranchych W., Sastry P. A., Volpel K., Loh B. A., Speert D. P. 1986; Fimbriae (pili): molecular basis of Pseudomonas aeruginosa adherence. Clinical and Investigative Medicine 9:113–118
    [Google Scholar]
  32. Pennington J. E., Wolf S. M., Puziss M. 1979; Summary of a workshop on infections in patients with cystic fibrosis. Journal of Infectious Diseases 140:252–256
    [Google Scholar]
  33. Plotkowski M. C., Beck G., Bernardo Filho M., Oliveria E. F. R., Hinnrasky J., Puchelle E. 1987; Evaluation of 99mTechnetium labelling effect on Pseudomonas aeruginosa surface properties. Annales de I’lnstitut Pasteur Microbiologie 138:415–426
    [Google Scholar]
  34. Plotkowski M. C., Beck G., Jacquot J., Puchelle E. 1989a; The frog palate mucosa as a model for studying bacterial adhesion to mucus-coated respiratory epithelium. Journal of Comparative Pathology 100:37–46
    [Google Scholar]
  35. Plotkowski M. C., Girod- Vaquez S., Hinnrasky J., Fuchey C., Ploton D., Puchelle E. 1989b; Ultrastructural comparative distribution of carbohydrates in human tracheal and frog palate mucosa using neuraminidase and lectin-colloidal gold complexes. Journal of Submicroscopic Cytology and Pathology (in press)
    [Google Scholar]
  36. Puchelle E., Petit A., Adnet J. J. 1984; Fine structure of the frog palate mucociliary epithelium. Journal of Submicroscopic Cytology 16:273–282
    [Google Scholar]
  37. Ramphal R., Pyle M. 1983; Adherence of mucoid and nonmucoid Pseudomonas aeruginosa to acid-injured tracheal epithelium. Infection and Immunity 41:345–351
    [Google Scholar]
  38. Ramphal R., Pier G. B. 1985; Role of Pseudomonas aeruginosa mucoid exopolysaccharide in adherence to tracheal cells. Infection and Immunity 47:1–4
    [Google Scholar]
  39. Ramphal R., Guay C., Pier G. B. 1987; Pseudomonas aeruginosa adhesins for tracheobronchial mucin. Infection and Immunity 55:600–603
    [Google Scholar]
  40. Reichert R. W., Das N. E., Zam Z. S. 1983; Adherence properties of Pseudomonas pili to epithelial cells of the human cornea. Current Eye Research 2:289–293
    [Google Scholar]
  41. Sharon N. 1987; Bacterial lectins, cell-cell recognition and infectious disease. FEES Letters 217:145–157
    [Google Scholar]
  42. Smallman L. A., Hill S. L., Stockley R. A. 1984; Reduction of ciliary beat frequency in vitro by sputum from patients with bronchiectasis: a serine protease effect. Thorax 39:663–667
    [Google Scholar]
  43. Stockley R. A., Burnett D. 1979; Alpha-1 -antitrypsin and leukocyte elastase in infected and noninfected sputum. American Review of Respiratory Disease 120:1081–1086
    [Google Scholar]
  44. Sturgess J. M. 1981; Mucus secretion and clearance in the pathogenesis of cystic fibrosis. Monographs in Pediatrics 14:60–74
    [Google Scholar]
  45. Suter S., Schaad U. B., Roux L., Nydegger U. E., Waldvogel F. A. 1984; Granulocyte neutral proteases and Pseudomonas elastase as possible causes of airway damage in patients with cystic fibrosis. Journal of Infectious Diseases 149:523–531
    [Google Scholar]
  46. Suter S., Schaad U. B., Morgenthaler J. J., Chevallier I., Schnebli H. P. 1988; Fibronectin-cleaving activity in bronchial secretions of patients with cystic fibrosis. Journal of Infectious Diseases 158:89–100
    [Google Scholar]
  47. Uhlenbruck G. 1987; Bacterial lectins: mediators of adhesion. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. Series A 263:497–508
    [Google Scholar]
  48. Woods D. E., Bass J. A., Johanson W. G., Strauss D. C. 1980a; Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients. Infection and Immunity 30:694–699
    [Google Scholar]
  49. Woods D. E., Strauss D. C., Johanson W. G., Berry V. K., Bass J. A. 1980b; Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infection and Immunity 29:1146–1151
    [Google Scholar]
  50. Woods D. E., Strauss D. C., Johanson W. G., Bass J. A. 1983; Factors influencing the adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Reviews of Infectious Diseases 5: suppl 5 S846–S851
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-30-4-285
Loading
/content/journal/jmm/10.1099/00222615-30-4-285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error