1887

Abstract

SUMMARY

Of 120 laboratory-maintained strains of and two of examined for haemolytic and lipolytic activity, 62 exhibited haemolytic activity alone, 20 of these showed haemolytic and lipolytic activity and 40 had neither activity. The strains showed both activities. The results indicated a relationship between haemolysin production and lipolytic activity which was not explained by the serotype of the organism. In addition, the following hydrolytic activities were detected in the cell-free growth media of strains Boldy and (formerly ) Type 5 (substrates acted upon are given in parentheses): acid phosphatase (4-nitrophenylphosphate, naphthyl phosphate, glycerophosphate, phosphorylcholine and GTP); neutral phosphatase (4-nitrophenylphosphate, naphthyl phosphate, phosphorycholine, NADP and UDPG); phosphodiesterase (-4-nitrophenylphosphate, ATP and NADP); NADase (NAD); phospholipase C (4-nitrophenylphosphoryl-choline, phosphatidyl choline and ethanolamine, and sphingomyelin); and lipase and esterase (triacetin, tributyrin, triolein, naphthyl-laurate, -myristate, -caprylate, -palmitate and -oleate, 4-nitrophenyl-acetate -laurate and Tween 80). The preparations also showed weak catalase activity. No evidence was found for the presence of RNAase, DNAase, peptidase/amidase, phosphoamidase, α-amylase, glucosidase, galactosidase, pyranosidase or glucose aminidase.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-30-2-111
1989-10-01
2022-12-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/30/2/medmicro-30-2-111.html?itemId=/content/journal/jmm/10.1099/00222615-30-2-111&mimeType=html&fmt=ahah

References

  1. Albaum H. G., Umbriet W. W. 1947; Differentiation between ribose-3-phosphate and ribose-5-phosphate by means of the orcinol-pentose reaction. Journal of Biological Chemistry 167:369–376
    [Google Scholar]
  2. Ames B. N. 1966; Assay of inorganic phosphate, total phosphate and phosphatase. In Neufield E. F., Ginsburg V. (eds) Methods in enzymology vol 8 Academic Press; New York: pp 115–118
    [Google Scholar]
  3. Baman S. I., Haque R. 1970; Production of multivalent extracellular filtrates of Staphylococcus aureus. Canadian Journal of Microbiology 16:1255–1261
    [Google Scholar]
  4. Barclay R., Threlfall D. R., Leighton I. 1989; Separation and properties of the haemolysins and extracellular enzymes of Listeria monocytogenes. Journal of Medical Microbiology 30:119–127
    [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
    [Google Scholar]
  6. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  7. Girard K. F., Sbarra A. J., Bardawil W. A. 1963; Serology of Listeria monocytogenes. 1. Characteristics of the soluble hemolysin. Journal of Bacteriology 85:349–355
    [Google Scholar]
  8. Harvey P. C., Faber J. E. 1941; Some biochemical reactions of the Listerella group (Abstract). Journal of Bacteriology 41:45–46
    [Google Scholar]
  9. Jenkins E. M., Watson B. B. 1971; Extracellular antigens from Listeria monocytogenes. 1. Purification and resolution of hemolytic and lipolytic antigens from culture filtrates of Listeria monocytogenes. Infection and Immunity 3:589–594
    [Google Scholar]
  10. Kaplan N. O., Colowick S. P., Nason A. 1951; Neurospora diphosphopyridine nucleotidase. Journal of Biological Chemistry 191:473–483
    [Google Scholar]
  11. Khan M. A., Seaman A., Woodbine M. 1972; Listeria monocytogenes—haemolysin: lecithinase. Acta Microbiologa Academiae Scientiarum Hungaricae 19:341–352
    [Google Scholar]
  12. Kramer P. A., Jones D. 1969; Media selective for Listeria monocytogenes. Journal of Applied Bacteriology 32:381–394
    [Google Scholar]
  13. Leighton I., Threlfall D. R., Oakley C. 1975; Phospholipase C activity in culture filtrates of Listeria monocytogenes Boldy. In Woodbine M. (ed) Problems of listeriosis. Proceedings of the VI International Symposium Leicester University Press; Leicester: pp 239–241
    [Google Scholar]
  14. Lowry O. H., Roseborough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. Njoku-Obi A. N., Jenkins E. M., Njoku-Obi J. C., Adams J., Covington V. 1963; Production and nature of Listeria monocytogenes hemolysins. Journal of Bacteriology 86:1–8
    [Google Scholar]
  16. Ogur M., Rosen G. 1950; The nucleic acids of plant tissues. I. The extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Archives of Biochemistry 25:262–276
    [Google Scholar]
  17. Patocka F., Mara M. 1973; Contribution to knowledge of factors participating in virulence of Listeria monocytogenes. 1. Isolation and properties of the biologically active complex E. Journal of Hygiene, Epidemiology, Microbiology and Immunology 17:457–468
    [Google Scholar]
  18. Schneider W. C., Fiscus W. G., Lawler J. B. 1966; Enzymic preparation of labelled phosphoryl choline, phosphoryl ethanolamine, cytidine diphosphate choline, deoxycitidine diphosphate choline, cytidine diphosphate ethanolamine, and deoxcytidine diphosphate ethanolamine. Analytical Biochemistry 14:121–134
    [Google Scholar]
  19. Seeliger H. P. R. 1961; Listeriosis. Hafner Publishing Co; New York:
    [Google Scholar]
  20. Siddique I. H., Lin I.-F., Chung R. A. 1974; Purification and characterisation of haemolysin produced by Listeria monocytogenes. American Journal of Veterinary Research 35:289–296
    [Google Scholar]
  21. Stanley N. F. 1949; Studies on Listeria monocytogenes. 1. Isolation of a monocytosis producing agent (MPA). Australian Journal of Experimental Biology and Medical Science 27:123–131
    [Google Scholar]
  22. Sword C. P., Kingdon G. C. 1971; Listeria monocytogenes toxin. In Kadis S. (eds) Microbial toxins—bacterial protein toxins Academic Press; New York and London: pp 357–377
    [Google Scholar]
  23. Yamakawa Y., Ohsaka A. 1977; Purification and some properties of phospholipase C (a-Toxin) of Clostridium perfringens. Journal of Biochemistry 81:115–126
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-30-2-111
Loading
/content/journal/jmm/10.1099/00222615-30-2-111
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error