1887

Abstract

SUMMARY

Lactic dehydrogenase, alkaline phosphatase and a magnesium-independent adenosine triphosphatase were assayed in normal, diarrhoeal and cholera stools. The diarrhoeas, examined bacteriologically, included bacillary dysentery, salmonellosis, amoebiasis and non-specific gastro-enteritis.

Alkaline phosphatase levels were found to be similar in normal and cholera stools, whereas lactic dehydrogenase was 30-fold higher and adenosine triphosphatase four-fold higher in cholera. The distribution of activity of lactic dehydrogenase was higher, and of the phosphatases lower in cholera than in the stools of bacterial infections showing evidence of tissue necrosis. The levels of activity of all three enzymes in cholera stool were found to reach a maximum between 9 and 17 hr after onset of purging.

It is suggested that these findings indicate an increased permeability of the intestinal epithelium in cholera, leading to a leak of cell enzymes into the intestinal lumen.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-3-3-453
1970-08-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/3/3/medmicro-3-3-453.html?itemId=/content/journal/jmm/10.1099/00222615-3-3-453&mimeType=html&fmt=ahah

References

  1. Bergmeyer H. U., Bernt E., Hess B. 1963 In Methods of enzymatic analysis. B. ed. by H. U. Bergmeyer, Weinheim p 736
    [Google Scholar]
  2. Crane K. 1966 Gastroenterology 50:254
    [Google Scholar]
  3. Crane K. 1968 A. Rev. Med 19:57
    [Google Scholar]
  4. Dammin G. J. 1965 Fedn Proc 24:35
    [Google Scholar]
  5. Dutt A. R. 1965 Ind. J. Med. Res 53:605
    [Google Scholar]
  6. Fernley H. N., Walker P. G. 1966 Biochem. J 99:P39
    [Google Scholar]
  7. Fiske C. H., Subbarow Y. 1925 J. Biol. Chem 66:375
    [Google Scholar]
  8. Fordtran M. D., Dietschy M. D. 1966 Gastroenterology 50:263
    [Google Scholar]
  9. Fresh J. W. 1965 Proc. Cholera Res. Symp Honolulu, U,. S.: Dept of Health Education and Welfare, Public Health Service; p 296
    [Google Scholar]
  10. Fuhrman G. J., Fuhrman F. A. 1960 Nature, Lond 188:71
    [Google Scholar]
  11. Gangarosa E. J., Sribhibhadh R., Sprinz H., Benyajati C., Kindel D., Beisel W. R., Halstead S. 1960; Proc. SEATO Conf. Cholera. Dacca p 76
    [Google Scholar]
  12. Gordon R. S. Jr 1960 Ibid p 54
    [Google Scholar]
  13. Hess B. 1963 Enzymes in blood plasma New York:
    [Google Scholar]
  14. Huber G. S. 1965 Proc. Cholera Res. Symp Honolulu, U,. S: Dept of Health Education and Welfare, Public Health Service; p 41
    [Google Scholar]
  15. Huber G. S., Phillips R. A. 1960 Proc. SEATO Conference on Cholera, Dacca p 37
    [Google Scholar]
  16. Iwert M. E., Deb B. C., Srivastava D. L., Burrows W. 1968 J. Infect. Dis 118:422
    [Google Scholar]
  17. Iwert M. E., Leitch G. J., Burrows W. 1967 Ibid 117:265
    [Google Scholar]
  18. Konttinez A., Rajasalmi M., Paloheimo J. 1964 Amer. J. Physiol 207:385
    [Google Scholar]
  19. Love A. H. G. 1965 Proc. Cholera Res. Symp Honolulu, U,. S: Dept of Health Education and Welfare, Public Health Service; p 144
    [Google Scholar]
  20. Mahajan R. G., Narayanaswami A. 1968 Proc. LV Ind. Sci. Congr Part III: p 173 B.8
    [Google Scholar]
  21. Melby J. C., Bossenmaier I. C., Egdahl R. H., Spink W. W. 1959 Lancet 1:441
    [Google Scholar]
  22. Moss D. W., Eaton R. 1966 Biochem. J 99:P19
    [Google Scholar]
  23. Onodera R., Tsuneyuki T., Kaichi A., Motoyoshi U. 1967 Tohoku J. Agric. Res 18:177 (quoted in). Biological Abstracts, 1968, 49, abstr no. 98915)
    [Google Scholar]
  24. Padykula H. A. 1961 Gastroenterology 40:735
    [Google Scholar]
  25. Phillips R. A. 1968 A. Rev. Med 19:69
    [Google Scholar]
  26. Raab W. 1967 Experientia 23:833
    [Google Scholar]
  27. Schwartz A., Bachelard H. S., McIlwain H. 1962 Biochem. J 84:626
    [Google Scholar]
  28. Shnitka T. K. 1960 Fedn Proc 19:897
    [Google Scholar]
  29. Skou J. C. 1957 Biochim. biophys. Acta 23:394
    [Google Scholar]
  30. Snedecor G. W. 1956; Statistical methods. Iowa pp 117 and 190
    [Google Scholar]
  31. Ussing H. H., Zerahn K. 1951 Acta physiol, scand 23:110
    [Google Scholar]
  32. Vesell E. S., Palmerio C. F. P., Frank E. D. 1960 Proc. Soc. Exp. Biol. Med 104:403
    [Google Scholar]
  33. Walker B. E., Leblond C. P. 1956 Physiol. Rev 36:255
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-3-3-453
Loading
/content/journal/jmm/10.1099/00222615-3-3-453
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error