1887

Abstract

Summary

The synergic relationship between and was examined in a model of intra-abdominal abscess formation. The addition of to in the fibrin clot inoculum increased abscess weight and residual numbers of in the abscess at 7 days. In a reciprocal fashion, was capable of enhancing persistence in abscesses. Neither heat-killed nor heat-killed was able to mimic the synergic effect of its live counterpart. Furthermore, culture filtrate was unable to reproduce the ability of live to act synergically with . For to act synergically with , it had to be inoculated locally with in the peritoneal cavity, indicating that an effect on systemic resistance by was an unlikely mechanism for the production of bacterial synergy. These studies suggest that the synergic relationship between bacteria in polymicrobial infections is a complex one, resulting from intimate interactions between bacteria and the host in the local milieu of the infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-29-4-269
1989-08-01
2022-05-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/29/4/medmicro-29-4-269.html?itemId=/content/journal/jmm/10.1099/00222615-29-4-269&mimeType=html&fmt=ahah

References

  1. Berne T. V., Yellin A. W., Appleman M. D., Heseltine P. N. R. 1982; Antibiotic management of surgically treated gangrenous or perforated appendicitis:comparison of gentamicin and clindamycin versus cefamandole versus cefoperazone. American Journal of Surgery 144:8–13
    [Google Scholar]
  2. Brook I., Hunter V., Walker R. I. 1984; Synergistic effect of Bacteroides, Clostridium, Fusobacterium, anaerobic cocci, and aerobic bacteria on mortality and induction of subcutaneous abscesses in mice. Journal of Infectious Diseases 149:924–928
    [Google Scholar]
  3. Delong T. G., Simmons R. L. 1982; Role of the lymphatics in bacterial clearance from early soft-tissue infection. Archives of Surgery 117:123–128
    [Google Scholar]
  4. Dunn D. L., Barke R. A., Ewald D. C., Simmons R. L. 1985; Effects of Escherichia coli and Bacteroides fragilis on peritoneal host defenses. Infection and Immunity 48:287–291
    [Google Scholar]
  5. Dunn D. L., Barke R. A., Ewald D. C., Simmons R. L. 1987; Macrophages and translymphatic absorption represent the first line of host defense of the peritoneal cavity. Archives of Surgery 122:105–110
    [Google Scholar]
  6. Gorbach S. L. 1984; Treatment of intraabdominal infection. American Journal of Medicine 76: Suppl 5A107–110
    [Google Scholar]
  7. Hagen J. C., Wood W. S., Hashimoto T. 1983; Experimental intraabdominal abscess formation by Escherichia coli and Bacteroidesfragilis. European Journal of Clinical Microbiology 2:43–49
    [Google Scholar]
  8. Hite K. E., Locke M., Hesseltine H. C. 1949; Synergism in experimental infection with nonsporulating ahaerobic bacteria. Journal of Infectious Diseases 84:1–9
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe Laboratory Manual. 4th edn Anaerobe Laboratory; Virginia Polytechnic Institute and State University, Blacksburg, VA:
    [Google Scholar]
  10. Ingham H. R., Sisson P. R., Middleton R. L., Narang H. K., Codd A. A., Selkon J. B. 1981; Phagocytosis and killing of bacteria in aerobic and anaerobic conditions. Journal of Medical Microbiology 14:391–399
    [Google Scholar]
  11. Jones G. R., Gemmell C. G. 1982; Impairment by Bacteroides species of opsonization and phagocytosis of enterobacteria. Journal of Medical Microbiology 15:351–361
    [Google Scholar]
  12. Kelly M. J. 1978; The quantitative and histological demonstration of pathogenic synergy between Escherichia coli and Bacteroides fragilis in guinea-pig wounds. Journal of Medical Microbiology 11:513–523
    [Google Scholar]
  13. Lorber B., Swenson R. M. 1975; The bacteriology of intra abdominal infection. Surgical Clinics of North America 55:1349–1354
    [Google Scholar]
  14. Mackowiak P. A. 1978; Microbial synergism in human infections (second of two parts). New England Journal of Medicine 298:83–87
    [Google Scholar]
  15. MacLaren D. M., Namavar F., Verweij-van Vught A. M. J. J., Vel W. A. C., Kaan J. A. 1984; Pathogenic synergy:mixed intraabdominal infections. Antonie van Leeuwenhoek 50:775–787
    [Google Scholar]
  16. Namavar F., Verweij A. M. J. J., Bal M., van Steenbergen T. J., de Graaf J., MacLaren D. M. 1983; Effect of anerobic bacteria on killing of Proteus mirabilis by human polymorphonuclear leukocytes. Infection and Immunity 40:930–935
    [Google Scholar]
  17. Onderdonk A. B., Bartlett J. G., Louie T. J., Sullivan-Siegler N., Gorbach S. L. 1976; Microbial synergy in experimental intraabdominal abscess. Infection and Immunity 13:22–26
    [Google Scholar]
  18. Onderdonk A. B., Kasper D. L., Cisneros R. L., Bartlett J. B. 1977; The capsular polysaccharide of Bacteroides fragilis as a virulence factor:comparison of the pathogenic potential of encapsulated and unencapsulated strains. Journal of Infectious Diseases 136:82–89
    [Google Scholar]
  19. Reznikov M., Finlay-Jones J. J., McDonald P. J. 1981; Effect of Bacteriodesfragilis on the peritoneal clearance of Escherichia coli in mice. Infection and Immunity 32:398–399
    [Google Scholar]
  20. Rodloff A. C., Becker J., Blanchard D. K., Klein T. W., Hahn H., Freidman H. 1986; Inhibition of macrophage phagocytosis by Bacteroides fragilis in vivo and in vitro. Infection and Immunity 52:488–492
    [Google Scholar]
  21. Rotstein O. D., Kao J. 1988; The spectrum of Escherichia coli-Bacteroides fragilis pathogenic synergy in an intraabdominal infection model. Canadian Journal of Microbiology 34:352–357
    [Google Scholar]
  22. Rotstein O. D., Pruett T. L., Simmons R. L. 1985; Lethal microbial synergism in intra-abdominal infections:Escherichia coli and Bacteroides fragilis. Archives of Surgery 120:146–151
    [Google Scholar]
  23. Rotstein O. D., Pruett T. L., Sorenson J. J., Fiegel V. D., Nelson R. D., Simmons R. L. 1986; A Bacteroides by-product inhibits human polymorphonuclear leukocyte function. Archives of Surgery 121:82–88
    [Google Scholar]
  24. Rotstein O. D., Pruett T. L., Wells C. L., Simmons R. L. 1987; The role of bacteroides encapsulation in the lethal synergy between Escherichia coli and Bacteroides species in a rat fibrin clot peritonitis model. Journal of Infection 15:135–146
    [Google Scholar]
  25. Shapiro M. E., Kasper D. L., Zaleznik D. F., Spriggs S., Onderdonk A. B., Finberg R. W. 1986; Cellular control of abscess formation:role of T cells in the regulation of abscesses formed in response to Bacteroides fragilis. Journal of Immunology 137:341–346
    [Google Scholar]
  26. Shapiro M. E., Onderdonk A. B., Kasper D. L., Finberg R. W. 1982; Cellular immunity to Bacteroides fragilis capsular polysaccharide. Journal of Experimental Medicine 155:1188–1197
    [Google Scholar]
  27. Van Dyke T. E., Bartholemew E., Genco R. J., Slots J., Levine M. J. 1982; Inhibition of neutrophil chemotaxis by soluble bacterial products. Journal of Periodontology 53:502–508
    [Google Scholar]
  28. Verweij-van Vught A. M. J. J., Namavar F., Sparrius M., Vel W. A. C., MacLaren D. M. 1985; Pathogenic synergy between Escherichia coli and Bacteroides fragilis: studies in an experimental mouse model. Journal of Medical Microbiology 19:325–331
    [Google Scholar]
  29. Wells C. L., Arland L. A., Simmons R. L., Rotstein O. D. 1985; In vivo bactericidal activity of Sch 34343 in Bacteroides fragilis abscesses and in Bacteroides fragilis-Escherichia coli abscesses. Journal of Antimicrobial Chemotherapy 15: Suppl C199–206
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-29-4-269
Loading
/content/journal/jmm/10.1099/00222615-29-4-269
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error