1887

Abstract

Summary

An IgM immunosorbent agglutination assay (ISAGA) was compared with a standard ELISA IgM test for the diagnosis of congenital toxoplasmosis. It was more sensitive, detecting all of five mothers of infected babies whereas the IgM ELISA was positive in two of three mothers tested at delivery and neither of two mothers referred 10–12 months after delivery. Five women infected in a previous pregnancy had IgM detectable by ISAGA in a subsequent pregnancy. The assays were comparable when sera from patients with past infection were tested or following toxoplasma-associated miscarriage or abortion. Four cord sera from congenitally-infected babies were positive by the ISAGA but only three of these were positive by ELISA for IgM. The ISAGA also detected IgM in another four sera from congenitally-infected babies referred late (10–18 months old); none were IgM positive by ELISA. The increased sensitivity of the ISAGA is an improvement in the diagnosis of congenital toxoplasmosis.

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/content/journal/jmm/10.1099/00222615-28-2-125
1989-02-01
2022-01-19
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References

  1. Daffos F. 1988; Prenatal management of 746 pregnancies at risk for congenital toxoplasmosis. New England Journal of Medicine 318:271–275
    [Google Scholar]
  2. Desmonts G., Couvreur J. 1974; Toxoplasmosis in pregnancy and its transmission to the fetus. Bulletin of the New York A cademy of Medicine 50:146–159
    [Google Scholar]
  3. Desmonts G., Daffos F., Forestier F., Capella-Pavlosky M., Thulliez Ph., Chartier M. 1985; Prenatal diagnosis of congenital toxoplasmosis. Lancet 1:500–504
    [Google Scholar]
  4. Desmonts G., Naot Y., Remington J. S. 1981; Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired toxoplasma infections. Journal of Clinical Microbiology 14:486–491
    [Google Scholar]
  5. Joss A. W. L., Skinner L. J., Chatterton J. M. W., Chisholm S. M., Williams H. D., Ho-Yen D. O. 1988; Simultaneous serological screening for congenital cytomegalovirus and toxoplasma infection. Public Health 102:409–417
    [Google Scholar]
  6. Koppe J. G., Loewer-Sieger D. H., de Roever-Bonnet H. 1986; Results of 20-year follow-up of congenital toxoplasmosis.
  7. Naot Y., Desmonts G., Remington J. S. 1981; IgM enzyme-linked immunosorbent assay test for the diagnosis of congenital toxoplasma infection. Journal of Pediatrics 98:32–36
    [Google Scholar]
  8. Remington J. S., Desmonts G. 1976; Toxoplasmosis. In Remington J. S., Klein J. O. (eds) Infectious diseases of the fetus and newborn infant W B Saunders Co; Philadelphia: pp 191–332
    [Google Scholar]
  9. Thorburn H., Williams H. 1972; A stable haemagglutinating antigen for detecting toxoplasma antibodies. Journal of Clinical Pathology 25:762–767
    [Google Scholar]
  10. Wielaard F. 1983; Diagnosis of acute toxoplasmosis by an enzyme immunoassay for specific immunoglobulin M antibodies. Journal of Clinical Microbiology 17:981–987
    [Google Scholar]
  11. Williams K. A. B., Scott J. M., Macfarlane D. E., Williamson J. M. W., Elias-Jones T. F., Williams H. 1981; Congenital toxoplasmosis: a prospective survey in the West of Scotland. Journal of Infection 3:219–229
    [Google Scholar]
  12. Wilson C. B., Remington J. S., Stagno S., Reynolds D. W. 1980; Development of adverse sequelae in children bom with subclinical congenital toxoplasma infection. Pediatrics 66:767–774
    [Google Scholar]
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