1887

Abstract

Summary

Infection of HEp-2 cells by enteropathogenic (EPEC) was examined by transmission and scanning electronmicroscopy. EPEC strains of serogroups O111:K58 and O55:K59 recently isolated from human patients did not exhibit enterotoxic activity, as judged by the Vero-cell and suckling-mouse assays, or invasive ability as judged by the Sereny test. These strains attached to and penetrated HEp-2 cells. Transmission electronmicroscopy showed bacteria in close contact with cell membranes 15 min after infection; later, intense swelling and budding of membranes and penetration of EPEC into the cell cytoplasm occurred. Intracellular bacteria were enclosed in membrane-bound vacuoles in the cell cytoplasm underlying localised adherence sites observed by light microscopy. Scanning electronmicroscopy showed morphologically altered membranes only at the sites of bacterial attachment. Bacteria inactivated by ultraviolet light were not internalised and cytochalasin B (≥ 10 mg/L) markedly inhibited uptake. These observations suggest that penetration of EPEC into HEp-2 cells occurs by an endocytic process in metabolically active bacteria.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-28-1-49
1989-01-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/28/1/medmicro-28-1-49.html?itemId=/content/journal/jmm/10.1099/00222615-28-1-49&mimeType=html&fmt=ahah

References

  1. Alberts B., Bray D., Lewis J., Raff M., Roberts K., Watson J. D. (eds) 1983; The cytoskeleton. In Molecular biology of the cell Garland Publishing Inc; New York: pp 549–609
    [Google Scholar]
  2. Allison A. C., Davies P., de Petris S. 1971; Role of contractile microfilaments in macrophage movement and endocytosis. Nature New Biology 232:153–155
    [Google Scholar]
  3. Andrade J. R. C., Canine G. A., Parentoni L. S., Santa Rosa M. R. 1984; Pili e aderencia para celulas em Escherichia coli enteropathogenicas. Revista de Microbiologia Sao Paulo 15:239–245
    [Google Scholar]
  4. Andrade J. R. C., Santa Rosa M. R. 1986a; Attachment and intracellular penetration of classic enteropathogenic Escherichia coli into Hep-2 cells. Revista de Microbiologia Sao Paulo 17:53–57
    [Google Scholar]
  5. Andrade J. R. C., Santa Rosa M. R. 1986a; Investigation on an adhesive property (localised adherence) characteristic of classical enteropathogenic serotypes of Escherichia coli. Revista de Microbiologia Sao Paulo 17:116–125
    [Google Scholar]
  6. Andrade J. R. C., Da Silva C. C. 1987; Endocytosis-inducer adhesins produced by enteropathogenic serogroups of Escherichia coli participate on bacterial attachment to infant enterocytes. Memorias do Instituto Oswaldo Cruz 82:87–90
    [Google Scholar]
  7. Baldini M. M., Kaper J. B., Levine M. M., Candy D. C. A., Moon H. W. 1983; Plasmid-mediated adhesion in enteropathogenic Escherichia coli. Journal of Pediatric Gastro-enterology and Nutrition 2:534–548
    [Google Scholar]
  8. Calabi O. 1970; In-vitro interaction of Shigella flexneri with leukocytes and HeLa cells. Journal of Infectious Diseases 122:1–9
    [Google Scholar]
  9. Coiro J. R. R., Weigl D. R., Kiselins J., Menezes H., Billota J. A. T. 1972; A new embedding medium (Polylite 8001) for biological material. Ciencia e Cultura 24:660–662
    [Google Scholar]
  10. Cravioto A., Gross R. J., Scotland S. M., Rowe B. 1979; An adhesive factor found in strains of Escherichia coli belonging to the traditional infantile enteropathogenic serotypes. Current Microbiology 3:95–99
    [Google Scholar]
  11. Echeverria P. D., Chang C. P., Smith D. 1976; Enterotoxigenicity and invasive capacity of “enteropathogenic” serotypes of Escherichia coli. Journal of Pediatrics 89:8–10
    [Google Scholar]
  12. Gerber D. F., Watkins H. M. S. 1961; Growth of Shigellae in monolayer tissue cultures. Journal of Bacteriology 82:815–822
    [Google Scholar]
  13. Gomes J. A., Rodrigues A. C., Simoes M., Serafim M. B., Pestana de Castro A. F. 1979; Simplification of methods for the production and storage of specimens to be tested for heat-stable enterotoxin of Escherichia coli. Journal of Clinical Microbiology 10:786–790
    [Google Scholar]
  14. Hale T. L., Bonventre P. F. 1979; Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infection and Immunity 24:879–886
    [Google Scholar]
  15. Hale T. L., Morris R. E., Bonventre P. F. 1979; Shigella infection of Henle intestinal epithelial cells: role of the host cell. Infection and Immunity 24:887–894
    [Google Scholar]
  16. Kihlström E., Edebo L. 1976; Association of viable and inactivated Salmonella typhimurium 395 MS and MR-10 with HeLa cells. Infection and Immunity 14:851–857
    [Google Scholar]
  17. Kihlström E., Nilsson L. 1977; Endocytosis of Salmonella typhimurium 395 MS and MR-10 by HeLa cells. Acta Pathologica et Microbiologica Scandinavica Section B. 85:322–328
    [Google Scholar]
  18. Klipstein F. A., Rowe B., Engert R. F., Short H. B., Gross R. J. 1978; Enterotoxigenicity of enteropathogenic serotypes of Escherichia coli isolated from infants with epidemic diarrhea. Infection and Immunity 21:171–178
    [Google Scholar]
  19. Knutton S., Williams P. H., Lloyd D. R., Candy D. C. A., McNeish A. S. 1984; Ultrastructural study of adherence to and penetration of cultured cells by two invasive Escherichia coli strains isolated from infants with enteritis. Infection and Immunity 44:599–608
    [Google Scholar]
  20. Knutton S., Baldini M. M., Kaper J. B., McNeish A. S. 1987; Role of plasmid-encoded adherence factors in adhesion of enteropathogenic Escherichia coli to HEp-2 cells. Infection and Immunity 55:78–85
    [Google Scholar]
  21. Konowalchuck J., Speirs J. I., Stavric S. 1977; Vero response to a cytotoxin of Escherichia coli. Infection and Immunity 18:775–779
    [Google Scholar]
  22. Levine M. M. 1978; Escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet 1:1119–1122
    [Google Scholar]
  23. Levine M. M. 1985; The diarrheal response of man to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. Journal of Infectious Diseases 152:550–559
    [Google Scholar]
  24. Levine M. M. 1987; Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, entero-hemorrhagic, and enteroadherent. Journal of Infectious Diseases 155:377–389
    [Google Scholar]
  25. Moon H. W., Whipp S. C., Argenzio R. A., Levine M. M., Giannella R. A. 1983; Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infection and Immunity 41:1340–1351
    [Google Scholar]
  26. Nataro J. P., Baldini M. M., Kaper J. B., Black R. E., Bravo N., Levine M. M. 1985; Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. Journal of Infectious Diseases 152:560–565
    [Google Scholar]
  27. O’Brien A. D., LaVeck G. D., Thompson M. R., Formal S. B. 1982; Production of Shigella dysenteriae type 1-like cytotoxin by Echerichia coli. Journal of Infectious Diseases 146:763–769
    [Google Scholar]
  28. Ogawa H. 1967; Virulence and epithelial cell invasiveness of dysentery bacilli. Japanese Journal of Medical Science and Biology 20:315–328
    [Google Scholar]
  29. Ogawa H., Nakamura A., Sakazaki R. 1968; Pathogenic properties of “entero-pathogenic” Escherichia coli from diarrheal children and adults. Japanese Journal of Medical Science and Biology 21:333–349
    [Google Scholar]
  30. Peeters J. E., Charlier G. J., Halen P. H. 1984; Pathogenicity of attaching effacing enteropathogenic Escherichia coli isolated from diarrheic suckling and weanling rabbits for newborn rabbits. Infection and Immunity 46:690–696
    [Google Scholar]
  31. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque strain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  32. Rothbaum R., McAdams A. J., Gianella R., Partin J. C. 1982; A clinicopathologic study of enterocyte-adherent Escherichia coli: a cause of protracted diarrhea in infants. Gastroenterology 83:441–454
    [Google Scholar]
  33. Scaletsky I. C. A., Silva M. L. M., Trabulsi L. R. 1984; Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infection and Immunity 45:534–536
    [Google Scholar]
  34. Serény B. 1955; Experimental Shigella keratoconjunctivitis. A preliminary report. Acta Microbiologica Academiae Scientiarum Hungaricae 2:293–296
    [Google Scholar]
  35. Staley T. E., Jones E. W., Corley L. D. 1969; Attachment and penetration of Escherichia coli into intestinal epithelium of the ileum in newborn pigs. American Journal of Pathology 56:371–392
    [Google Scholar]
  36. Ulshen M. H., Rollo J. L. 1980; Pathogenesis of Escherichia coli gastroenteritis in man—another mechanism. New England Journal of Medicine 302:99–101
    [Google Scholar]
  37. WHO Scientific Working Group 1980; Escherichia coli diarrhoea. Bulletin of the World Health Organization 58:23–36
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-28-1-49
Loading
/content/journal/jmm/10.1099/00222615-28-1-49
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error