1887

Abstract

Summary

Thirty-four isolates of methicillin-resistant (MRSA) from patients in Glasgow Royal Infirmary were studied. Whole-cell protein profiles obtained by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) were compared with banding patterns produced by immunoblots of exported proteins. Human plasma was used as a source of staphylococcal antibodies for the immunoblots. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of MRSA. Reproducible banding patterns were obtained by immunoblots of exported proteins. Analyses of immunoblots by use of the Dice coefficient demonstrated that isolates of MRSA could be divided into two main groups. Immunoblots of exported proteins provided a rapid, reproducible and sensitive method for characterisation of MRSA.

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1989-01-01
2022-05-20
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References

  1. Barber M. 1961; Methicillin-resistant staphylococci. Journal of Clinical Pathology 14:385–393
    [Google Scholar]
  2. Bradley J. M., Noone P., Townsend D. E., Grubb W. B. 1985; Methicillin-resistant Staphylococcus aureus in a London hospital. Lancet 1:1493–1495
    [Google Scholar]
  3. Branger C., Goullet P. 1987; Esterase electrophoretic polymorphisms of methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus. Journal of Medical Microbiology 24:275–281
    [Google Scholar]
  4. Burnette W. N. 1981; “Western Blotting”: electrophoretic transfer of proteins from sodium dodecyl sulphate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  5. Burnie J. P., Matthews R. C. 1987; Immunoblot analysis: a new method for fingerprinting hospital pathogens. Journal of Immunological Methods 100:41–46
    [Google Scholar]
  6. Casewell M. W. 1986; Epidemiology and control of the “modern” methicillin-resistant Staphylococcus aureus. Journal of Hospital Infection 7: Suppl A 1–11
    [Google Scholar]
  7. de Saxe M., Cooke M., Mayon-White R., Galbraith N. S., Casewell M. 1983; Methicillin-resistant Staphylococcus aureus in the UK. Communicable Diseases Report 83/36:3
    [Google Scholar]
  8. Dice L. R. 1945; Measures of the amount of ecological association between species. Ecology 26:297–302
    [Google Scholar]
  9. Dowd G., Cafferkey M., Dougan G. 1983; Gentamicin and methicillin resistant Staphylococcus aureus in Dublin hospitals: molecular studies. Journal of Medical Microbiology 16:129–138
    [Google Scholar]
  10. Gelmi M. 1987; Antibiotic resistance and plasmids from Staphylococcus aureus from Italian hospitals. Journal of Medical Microbiology 23:111–118
    [Google Scholar]
  11. Gillespie M. T., May J. W., Skurray R. A. 1984; Antibiotic susceptibilities and plasmid profiles of nosocomial methicillin-resistant Staphylococcus aureus:a retrospective study. Journal of Medical Microbiology 17:295–310
    [Google Scholar]
  12. Grieble H. G., Krause S. L., Pappas S. A., Dicostanzo M. B. 1981; The prevalence of high-level methicillin-resistance in multiply resistant hospital staphylococci. Medicine (Baltimore) 60:62–69
    [Google Scholar]
  13. Jevons M. P. 1961; “Celbenin”-resistant staphylococci. British Medical Journal 1:124–125
    [Google Scholar]
  14. Krikler S. J., Pennington T. H., Petrie D. 1986; Typing of strains of Staphylococcus aureus by Western blot analysis of culture supemates. Journal of Medical Microbiology 21:169–171
    [Google Scholar]
  15. Lacey R. W., Stokes A. 1979; Studies on recently isolated cultures of methicillin-resistant Staphylococcus aureus. Journal of General Microbiology 114:329–339
    [Google Scholar]
  16. Lee W., Burnie J. P. 1988; Fingerprinting methicillin-resistant Staphylococcus aureus by the immunoblot technique. Journal of Medical Microbiology 25:261–268
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  18. Locksley R. M. 1982; Multiply antibiotic-resistant Staphylococcus aureus: introduction, transmission and evolution of nosocomial infection. Annals of Internal Medicine 97:317–324
    [Google Scholar]
  19. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  20. Lyon B. R., Iuorio J. L., May J. W., Skurray R. A. 1984; Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. Journal of Medical Microbiology 17:79–89
    [Google Scholar]
  21. Marples R. R., Cooke E. M. 1985; Report of workshop on methicillin-resistant Staphylococcus aureus held at the headquarters of the Public Health Laboratory Service on 8 Jan 1985. Journal of Hospital Infection 6:342–348
    [Google Scholar]
  22. Marples R. R., Richardson J. F., de Saxe M. J. 1986; Bacteriological characters of strains of Staphylococcus aureus submitted to a reference laboratory related to methicillin resistance. Journal of Hygiene 96:217–223
    [Google Scholar]
  23. Pavillard R. 1982; Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Medical Journal of Australia 1:451–454
    [Google Scholar]
  24. Peacock J. E., Moorman D. R., Wenzel R. P., Mandell G. L. 1981; Methicillin-resistant Staphylococcus aureus: microbiologic characteristics, antimicrobial susceptibilities, and assessment of virulence of an epidemic strain. Journal of Infectious Diseases 144:575–582
    [Google Scholar]
  25. Poxton I. R., Aronsson B., Mollby R., Nord C. E., Collee J. G. 1984; Immunochemical fingerprinting of Clostridium difficile strains isolated from an outbreak of antibiotic-associated colitis and diarrhoea. Journal of Medical Microbiology 17:317–324
    [Google Scholar]
  26. Richardson J. F., Chittasobhon N., Marples R. R. 1988; Supplementary phages for the investigation of strains of methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology 25:67–74
    [Google Scholar]
  27. Stephenson J. R., Crook S. J., Tabaqchali S. 1986; New method for typing Staphylococcus aureus resistant to methicillin based on sulphur-35 methionine labelled proteins: its application in an outbreak. British Medical Journal 293:581–583
    [Google Scholar]
  28. Vickery A. M., Beard-Pegler M. A., Rountree P. M. 1983; Strain differentiation in methicillin-resistant Staphylococcus aureus. Pathology 15:235–240
    [Google Scholar]
  29. Vickery A. M., Beard-Pegler M. A., Stubbs E. 1986; Phage-typing patterns and lysogenicity of methicillin-resistant strains of Staphylococcus aureus from Sydney Australia 1965 85. Journal of Medical Microbiology 22:209–216
    [Google Scholar]
  30. Witte W., Braulke C. 1987; Multiply-resistant and methicillin-resistant Staphylococcus aureus strains isolated in the German Democratic Republic in 1985 and 1986. Epidemiology and Infection 99:603–612
    [Google Scholar]
  31. Witte W., Nguyen V. D., Dunnhaupt K. 1986; Methicillin-resistant Staphylococcus aureus (MRSA) in the German Democratic Republic. Incidence and strain-characteristics. Journal of Hygiene, Epidemiology, Microbiology and Immunology Prague 30:185–193
    [Google Scholar]
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