To obtain monoclonal antibodies (MAbs) directed preferentially against the pathogenic phase of , mice were immunised with germ tubes of serotype A, strain VW.32, killed by exposure to ultraviolet (UV) irradiation. Fusions were performed either by the standard chemical procedure with polyethylene glycol, or by electric discharge following linkage of the myeloma and lymphocyte cells with a Concanavalin A-mannoprotein bridge. The preliminary characteristics of one MAb obtained from each of these fusions are described. An IgM antibody (3B7) obtained from the chemical fusion reacted with a polysaccharide antigen that was heterogeneously distributed on both in-vitro and in-vivo forms of This MAb agglutinated different strains of irrespective of their serotype. An IgG antibody (3G6) that had been obtained from the electric fusion was found to react with a proteinaceous antigen located only on the germ tubes of strain VW.32. However, MAb 3G6 displayed strong reactivity against all growth forms of and reactivity extended to other strains.


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