1887

Abstract

SUMMARY

Clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of are described. The organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (. 1 mm) colonies on MacConkey agar. The organisms failed to grow in a minimal medium supplemented with sodium sulphate but grew when the medium was supplemented with cysteine sulphinic acid, sodium sulphide or L-cysteine. The smallest amount of cysteine required for optimal growth in a chemically denned medium was 20 mg/L. Cysteine-requiring strains of had previously been shown to require a similar amount of cysteine and to be unable to reduce sulphate to sulphite; this suggests a common influence in the selection of cysteine auxotrophs . However, the amino acid inhibited the growth of at concentrations which only slightly altered growth of the strains. Problems with the isolation, identification and sensitivity testing of cysteine-requiring were also observed and methods by which these may be minimised are suggested.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-26-3-211
1988-07-01
2022-05-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/26/3/medmicro-26-3-211.html?itemId=/content/journal/jmm/10.1099/00222615-26-3-211&mimeType=html&fmt=ahah

References

  1. Bell S. M. 1975; The CDS disc method of antibiotic sensitivity testing (calibrated dichotomous sensitivity test). Pathology 7: Suppl 1–48
    [Google Scholar]
  2. Bevis T. D. 1968; A modified electrolyte deficient culture medium. Journal of Medical Laboratory Technology 25:38–41
    [Google Scholar]
  3. Borderon E., Horodniceanu T. 1978; Metabolically deficient dwarf-colony mutants of Escherichia coli: deficiency and resistance to antibiotics of strains isolated from urine culture. Journal of Clinical Microbiology 8:629–634
    [Google Scholar]
  4. Brigham M. P., Stein W. H., Moore S. 1960; The concentrations of cysteine and cystine in human blood plasma. Journal of Clinical Investigation 39:1633–1638
    [Google Scholar]
  5. Cowan S. T. 1974; Cowan and Steel’s Manual for the identification of medical bacteria. 2nd ed Cambridge University Press; Cambridge: pp 103–110
    [Google Scholar]
  6. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli orvitamin B. 2 . Journal of Bacteriology 60:17–28
    [Google Scholar]
  7. Gillespie W. A. 1952; Biochemical mutants of coliform bacilli in infections of the urinary tract. Journal of Pathology and Bacteriology 64:551–557
    [Google Scholar]
  8. Jones-Mortimer M. C. 1968; Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping of cysteine-less mutants of E. coli K12. Biochemical Journal 110:589–595
    [Google Scholar]
  9. Leigh D. A., Williams J. D. 1964; Method for the detection of significant bacteriuria in large groups of patients. Journal of Clinical Pathology 17:498–503
    [Google Scholar]
  10. Mackey J. P., Sandys G. H. 1966; Diagnosis of urinary infections. British Medical Journal 1:1173
    [Google Scholar]
  11. Mclver C. J., Tapsall J. W. 1987; Cysteine requirements of naturally occurring cysteine auxotrophs of Escherichia coli. Pathology 19:361–363
    [Google Scholar]
  12. Miles A. A., Misra S. S., Irwin E. D. 1938; The estimation of the bactericidal power of the blood. Journal of Hygiene 38:732–749
    [Google Scholar]
  13. Nagy Z., Kari C., Hemadi F. 1969; Growth of Escherichia coli cells in the presence of cysteine on sulphate-deficient media. Archiv fur Mikrobiologie 65:391–400
    [Google Scholar]
  14. Pasternak C. A. 1962; Sulphate activation and its control in Escherichia coli and Bacillus subtilis. Biochemical Journal 85:44–49
    [Google Scholar]
  15. Tapsall J. W., Mclver C. J. 1986; Septicaemia caused by cysteine-requiring isolates of Escherichia coli. Journal of Medical Microbiology 22:379–382
    [Google Scholar]
  16. Tapsall J. W., Wilson E., Harper J. 1974; Thymine dependent strains of Escherichia coli selected by trimethoprim-sulpha-methoxazole therapy. Pathology 6:161–167
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-26-3-211
Loading
/content/journal/jmm/10.1099/00222615-26-3-211
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error