1887

Abstract

SUMMARY

A competitive enzyme-linked immunosorbent assay (CETPIA) was compared with the standard serological tests for syphilis. Of 3081 serum samples submitted, 2883 gave negative results in the CETPIA and the routine screening tests. Positive results were obtained in the CETPIA and in one or more of the specific treponemal tests with 115 samples. Discrepancies in the results of the CETPIA and standard serological tests were found with 83 serum samples, most of these were attributed to biological false positive reactions in the Venereal Disease Research Laboratory (VDRL) test. CETPIA may have a role in the serological diagnosis of syphilis.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-26-2-153
1988-06-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jmm/26/2/medmicro-26-2-153.html?itemId=/content/journal/jmm/10.1099/00222615-26-2-153&mimeType=html&fmt=ahah

References

  1. Engvall E., Perlmann P. 1971; Enzyme-linked Immunosorbent Assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871–874
    [Google Scholar]
  2. Engvall E., Perlmann P. 1972; Enzyme-linked Immunosorbent Assay, ELISA. Ill Quantification of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  3. Farshy C. E., Hunter E. F., Helsel L. O., Larsen S. A. 1985; Four-step enzyme-linked immunosorbent assay for detection of Treponema pallidum antibody. Journal of Clinical Microbiology 21:387–389
    [Google Scholar]
  4. Lindenschmidt Laufs R., Muller F. 1983; Microenzyme-linked immunosorbent assay for the detection of specific IgM antibodies in human syphilis. British Journal of Venereal Diseases 59:151–156
    [Google Scholar]
  5. Radolf J. D., Lemhardt E. B., Fehniger T. E., Lovett M. A. 1986; Serodiagnosis of syphilis by enzyme-linked Immunosorbent Assay with purified Recombinant Treponema pallidum Antigen 4D. Journal of Infectious Diseases 153:1023–1027
    [Google Scholar]
  6. Veldkamp J., Visser A. M. 1975; Application of the Enzyme-linked Immunosorbent Assay (ELISA) in the Serodiagnosis of syphilis. British Journal of Venereal Diseases 51:227–231
    [Google Scholar]
  7. Wilkinson A. E. 1972; Serological Tests for Syphilis. In Laboratory diagnosis of venereal disease, Public Health Laboratory Service Monograph Series No. 1 London: HMSO, pp 1–24
    [Google Scholar]
  8. Wreghitt T. G., Tedder R. S., Ferns R. B. 1984; Antibody Assays for Varicella-Zoster Virus: Comparison of competitive enzyme-linked immunosorbent assay (ELISA), competitive radioimmunoassay (RIA), complement fixation, and indirect immunofluorescence assays. Journal of Medical Virology 13:361–370
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-26-2-153
Loading
/content/journal/jmm/10.1099/00222615-26-2-153
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error