1887

Abstract

Summary.

Fimbriae-like filaments were demonstrated on the surface of , serotype 1.3, by negative staining and electronmicroscopy. Immuno-electronmicroscopy with a monoclonal antibody specific for strains possessing agglutinogen 3, and colloidal gold, gave strong labelling of these structures. However, incubation with adsorbed polyclonal anti-agglutinogen 3 serum gave only weak labelling of the distal parts of the filaments and of the bacterial surface. The different binding patterns of the two antisera suggested that the epitopes involved were dissimilar. Thus, agglutinogen 3, as defined by conventional adsorbed sera, appeared to be associated with the fimbriae-like structures but was not necessarily identical to the fimbrial subunit protein. The monoclonal antibody, however, was more likely directed against the subunits of the fimbriae-like structures on serotype 1.3 bacteria.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-4-285
1988-04-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/4/medmicro-25-4-285.html?itemId=/content/journal/jmm/10.1099/00222615-25-4-285&mimeType=html&fmt=ahah

References

  1. Ashworth L A E, Dowsett A B, Irons L I, Robinson A. 1985; The location of surface antigens of Bordetella pertussis by immuno-electronmicroscopy. Developments in Biological Standardization 61:143–151
    [Google Scholar]
  2. Ashworth L A E, Irons L I, Dowsett A B. 1982; Antigenic relationship between serotype-specific agglutinogen and fimbriae of Bordetella pertussis. Infection and Immunity 37:1278–1281
    [Google Scholar]
  3. Carter E J, Preston N W. 1984; Association between Bordetella pertussis agglutinogen 2 and fimbriae. Journal of Medical Microbiology 18:87–94
    [Google Scholar]
  4. Cowell J L, Urisu A, Zhang J M, Steven A C, Manclark C R. 1986; The filamentous hemagglutinin and fimbriae of Bordetella pertussis: Properties and roles in attachment. Leive L. Microbiology-1986 American Society for Microbiology; Washington D.C:55–58
    [Google Scholar]
  5. Fredriksen J H, Freholm L O, Kjennerud U. 1987; The specificity of antisera against Bordetellapertussis examined by bacterial agglutination. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Sect B 95:363–369
    [Google Scholar]
  6. Fredriksen J H, Fraholm L O, Paulsen B S. 1985; Purification and preliminary characterization of agglutinogen 3 from Bordetella pertussis. Developments in Biological Standardization 61:187–196
    [Google Scholar]
  7. Lothe R A, Freholm L O, Westre G, Kjennerud U. 1985; Stainer and Scholte's pertussis medium with an alternative buffer. Journal of Biological Standardization 13:129–134
    [Google Scholar]
  8. Miller E. 1986; Progress towards a new pertussis vaccine. British Medical Journal 292:1348–1350
    [Google Scholar]
  9. Namork E, Johansen B V. 1982; Surface activation of carbon film supports for biological electron microscopy. Ultramicroscopy 7:321–330
    [Google Scholar]
  10. Preston N W. 1963; Type-specific immunity against whooping-cough. British Medical Journal 2:724–726
    [Google Scholar]
  11. Preston N W. 1985; Essential immunogens in human pertussis: The role of fimbriae. Developments in Biological Standardization 61:137–141
    [Google Scholar]
  12. Preston N W, Surapatana N, Carter E J. 1982; A reappraisal of serotype factors 4, 5 and 6 of Bordetella pertussis. Journal of Hygiene 88:39–46
    [Google Scholar]
  13. Robinson A, Irons L I, Ashworth L A E. 1985; Pertussis vaccine : present status and future prospects. Vaccine 3:11–22
    [Google Scholar]
  14. Steven A C, Bisher M E, Trus B L, Thomas D, Zhang J M, Cowell J L. 1986; Helical structure of Bordetella pertussis fimbriae. Journal of Bacteriology 167:968–974
    [Google Scholar]
  15. Weiss A A, Hewlett E L. 1986; Virulence factors of Bordetella pertussis. Annual Review of Microbiology 40:661–686
    [Google Scholar]
  16. World Health Organization, Expert Committee on Biological Standardization 1979; Requirements for pertussis vaccine. Thirtieth Report. WHO, Technical Report Series 63860–80
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-4-285
Loading
/content/journal/jmm/10.1099/00222615-25-4-285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error