1887

Abstract

Summary

The rate of incorporation of hypoxanthine was measured in suspensions of , with and without added anti-leprosy agents. Dapsone, clofazamine and brodimoprim, as well as other benzylpyrimidines, inhibited hypoxanthine incorporation, and their minimum inhibitory concentrations for incorporation with intact were near the minimum inhibitory concentrations at which the agents have antibacterial effects. At sub-inhibitory concentrations for hypoxanthine incorporation, some combinations of benzylpyrimidines and dapsone were inhibitory, suggesting that synergic effects of anti-leprosy agents might also be detected by the inhibition of hypoxanthine incorporation. Thus, demonstration of inhibition of hypoxanthine incorporation in could be a rapid method for screening anti-leprosy agents and especially for preliminary testing of new, potential anti-leprosy agents. The rate of hypoxanthine incorporation was generally lower in suspensions of with lower viability, but it was not proportional to viability so the technique would not be suitable for accurate determination of viability.

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1988-03-01
2022-01-28
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