1887

Abstract

Summary

Clinical isolates of harbouring resistance (R) plasmids of mol. wts 4·4 × 10 (Asian) or 3·2 × 10 (African) were grown in prolonged glucose-limited continuous culture to determine the segregation efficiency of each type of plasmid and their expression of penicillinase activity in the absence of antibiotic selective pressure. One strain contained the African plasmid and cryptic and conjugative plasmids, which were all retained after 96 generations in the chemostat. By contrast, the other strain lost all plasmids after 100 generations. Both strains showed increased sensitivity to a range of antibiotics, particularly to the penicillins. Loss of penicillinase activity as minimal inhibitory concentration decreased was confirmed for both strains by assaying the enzyme spectrophotometrically. Activity decreased with the number of generations and none was detectable at the time of complete plasmid loss. This decrease was apparently due to individual bacteria ceasing to produce enzyme rather than a gradual decline in production by the whole population. The sensitivities to a broad range of antibiotics also generally increased during glucose-limited growth, but one strain became more resistant to clindamycin and the other to tetracycline.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-24-4-351
1987-12-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/24/4/medmicro-24-4-351.html?itemId=/content/journal/jmm/10.1099/00222615-24-4-351&mimeType=html&fmt=ahah

References

  1. Aalen R B, Gundersen W B. 1985; Polypeptides encoded by cryptic plasmids from Neisseria gonorrhoeae. Plasmid 14:209–216
    [Google Scholar]
  2. Aranha H, Strachan R C, Arceneaux J E L, Byers B R. 1982; Effect of trace metals on growth of Streptococcus mutatis in a teflon chemostat. Infection and Immunity 35:456–460
    [Google Scholar]
  3. Arya O P. 1984; Epidemiology of penicillinase-producing Neisseria gonorrhoeae in Liverpool from 1977 to 1982. Journalof Infection 8:70–83
    [Google Scholar]
  4. Ashford W A, Golash R G, Hemming V G. 1976; Penicillinase-producing Neisseria gonorrhoeae. Lancet 2:657–658
    [Google Scholar]
  5. Bergstrom S, Norlander L, Norqvist A, Normark S. 1978; Contribution of a TEM-1-like beta-lactamase to penicillin resistance in Neisseria gonorrhoeae. Antimicrobial Agents and Chemotherapy 13:618–623
    [Google Scholar]
  6. Bull A T. 1980; Biodegradation: some attitudes and strategies of micro-organisms and microbiologists. Ellwood D C. Contemporary microbial ecology Academic Press; London:107–136
    [Google Scholar]
  7. Cannon J G, Sparling P F. 1984; The genetics of the gonococcus. Annual Review of Microbiology 38:111–133
    [Google Scholar]
  8. Dougan G, Fairweather N. 1985; Genetic analysis of gram-positive toxin determinants: the impact of new technologies. Microbiological Sciences 2:144–147
    [Google Scholar]
  9. Elwell L P, Shipley P L. 1980; Plasmid-mediated factors associated with virulence of bacteria to animals. Annual Review of Microbiology 34:465–496
    [Google Scholar]
  10. Genco C A, Knapp J S, Clark V L. 1984; Conjugation of plasmids of Neisseria gonorrhoeae to other Neisseria species: potential reservoirs for the β-lactamase plasmid. Journal of Infectious Diseases 150:397–401
    [Google Scholar]
  11. Hardman D J, Gowland P C. 1985; Large plasmids in bacteria. 1. A survey of associated phenotypes. Microbiological Sciences 2:90–94
    [Google Scholar]
  12. Harris T J R, Emtage J S. 1986; Expression of heterologous genes in Escherichia coli. Microbiological Sciences 3:28–31
    [Google Scholar]
  13. Hintermann G, Fischer H-M, Crameri R, Hiitter R. 1981; Simple procedure for distinguishing CCC, OC and L forms of plasmid DNA by agarose gel electrophoresis. Plasmid 5:371–373
    [Google Scholar]
  14. Holmes D S, Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  15. Jephcott A E, Reyn A, Birch-Andersen A. 1971; Neisseria gonorrhoeae III. Demonstration of presumed appendages to cells from different colony types. Acta Pathologica et Microbiologica Scandinavica Section B 78:437–439
    [Google Scholar]
  16. Keevil C W, Major N C, Davies D B, Robinson A. 1986; Physiology and virulence determinants of Neisseria gonorrhoeae grown in glucose-, oxygen- or cystine-limited continuous culture. Journal of General Microbiology 132:3289–3302
    [Google Scholar]
  17. Macrina F L, Kopecko D J, Jones K R, Ayers D J, McCowen S M. 1978; A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1:417–420
    [Google Scholar]
  18. Manchee R J, Wiblin C N, Robinson A. 1980; Growth of Neisseria gonorrhoeae in continuous culture. FEMS Microbiology Letters 7:115–118
    [Google Scholar]
  19. Novotny P, Cownley K. 1979; Effect of growth conditions on the composition and stability of the outer membrane of Bordetella pertussis. Manclark C R, Hill J C. International symposium on pertussis Department of Health, Education and Welfare Publication no. 79-1830 US Government Printing Office; Washington, DC:99–123
    [Google Scholar]
  20. O’Callaghan C H, Morris A, Kirby S M, Shingler AH. 1972; Novel method for the detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrobial Agents and Chemotherapy 1:283–288
    [Google Scholar]
  21. Parker W S. 1946; Resistant gonococci. Lancet 2:850
    [Google Scholar]
  22. Percival A J E. 1976; Penicillinase-producing gonococci in Liverpool. Lancet 2:1379–1382
    [Google Scholar]
  23. Pestka S. 1971; Inhibitors of ribosome functions. Annual Review of Microbiology 25:487–562
    [Google Scholar]
  24. Primrose S B, Derbyshire P, Jones I M, Robinson A, Ellwood D C. 1982; The application of continuous culture to the study of plasmid stability. Dean A C R. Continuous culture 8: biotechnology, medicine and the environment Ellis Horwood; Chichester:213–238
    [Google Scholar]
  25. Roberts M, Elwell L P, Falkow S. 1977; Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. Journal of Bacteriology 131:557–563
    [Google Scholar]
  26. Sox T E, Mohammed W, Blackman E, Biswas G, Sparling P F. 1978; Conjugative plasmids in Neisseria gonorrhoeae. Journal of Bacteriology 134:278–286
    [Google Scholar]
  27. Sparling P F. 1979; Treatment of gonorrhoea: what effect will antibiotic resistance have in the future?. Sexually Transmitted Diseases 6:120–125
    [Google Scholar]
  28. Swanson J. 1978; Studies on gonococcus infection. XII. Colony color and opacity variants on gonococci. Infection and Immunity 19:320–331
    [Google Scholar]
  29. Van Klingeren B, Van Wijngaarden L J, Dessens Kroon M, Van Embden J D A. 1983; Penicillinase-producing gonococci in the Netherlands in 1981. Journal of Antimicrobial Chemotherapy 11:15–20
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-24-4-351
Loading
/content/journal/jmm/10.1099/00222615-24-4-351
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error