Skip to content
1887

Abstract

Summary.

A method for typing species of by S-labelling and sodium-dodecyl sulphate polyacrylamide-gel electrophoresis (SDS-PAGE) of total soluble proteins is described. Radiolabelled-protein profiles from different strains were compared by use of the Dice coefficient. Typability was 100% and discrimination of pairs of differing strains was satisfactory with acceptable reproducibility. The results also yielded information on subgroups of phenotypic species.

Erratum

A correction has been published for this content:
Erratum
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-24-2-113
1987-09-01
2026-03-09

Metrics

Loading full text...

Full text loading...

References

  1. Adams D, Atkinson H M, Woods W H. 1983; Aeromonas hydrophila typing scheme based on patterns of agglutination with erythrocytes and yeast cells. Journal of Clinical Microbiology 17:422–427
    [Google Scholar]
  2. Barer M R, Millership S E, Tabaqchali S. 1986; Relationship of toxin production to species in the genus Aeromonas. Journal of Medical Microbiology 22:303–309
    [Google Scholar]
  3. Burke V, Robinson J, Gracey M, Peterson D, Partridge K. 1984; Isolation of Aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates. Applied and Environmental Microbiology 48:361–366
    [Google Scholar]
  4. Cookson B D, Houang E T, Lee J V. 1984; The use of a biotyping system to investigate an unusual clustering of bacteraemias caused by Aeromonas species. Journal of Hospital Infection 5:205–209
    [Google Scholar]
  5. Delabre M, Bianchi A, Véron M. 1973; Étude critique de méthodes de taxonomie numérique. Application a une classification de bactéries aquicoles. Annales de Microbiologie 124A:489–506
    [Google Scholar]
  6. Dice L R. 1945; Measures of the amount of ecologic association between species. Ecology 26:297–302
    [Google Scholar]
  7. von Graevenitz A. 1984; Aeromonas and Plesiomonas. Ellner P D. Infectious diarrhoeal diseases Microbiology series vol. 12 Marcel Dekker; New York:59–75
    [Google Scholar]
  8. Laemmli U K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  9. Millership S E, Curnow S R, Chattopadhyay B. 1983; Faecal carriage rate of Aeromonas hydrophila. Journal of Clinical Pathology 36:920–923
    [Google Scholar]
  10. Picard B, Goullet P. 1984; Esterase electrophoresis: a new epidemiological screening test for Aeromonas hydrophila hospital infection. Journal of Hospital Infection 5:335–337
    [Google Scholar]
  11. Popoff M Y, Coynault C, Kiredjian M, Lemelin M. 1981; Polynucleotide sequence relatedness among motile Aeromonas species. Current Microbiology 5:109–114
    [Google Scholar]
  12. Stephenson J R, Crook S J, Tabaqchali S. 1986; New method for typing Staphylococcus aureus resistant to methicillin based on sulphur 35 methionine labelled proteins; its application in an outbreak. British Medical Journal 293:581–583
    [Google Scholar]
  13. Stephenson J R, Tabaqchali S. 1986; New method for typing coagulase negative staphylococci. Journal of Clinical Pathology 39:1271–1275
    [Google Scholar]
  14. Tabaqchali S, Holland D, O’Farrell S, Silman R. 1984; Typing scheme for Clostridium difficile: its application in clinical and epidemiological studies. Lancet 1:935–938
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-24-2-113
Loading
/content/journal/jmm/10.1099/00222615-24-2-113
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error