1887

Abstract

Summary

Spleen cells from mice immunised with group-A type-M15 streptococci and boosted with purified IgG Fc-receptor (FcR) from this type were fused with Sp 2/0 mouse myeloma cells. The resulting hybridomas were screened by ELISA for antibody production. Two IgM-secreting cell lines were selected. The monoclonal antibodies and ascites fluids inhibited the binding of I-labelled human IgG and IgG Fc-fragments to group-A type-M15 streptococci. The monoclonal antibodies also displaced purified FcR towards the anode in electrophoresis. They opsonised group-A type M-15 streptococci for phagocytosis by human granulocytes in the presence of fresh human serum. It was concluded that FcR is important for group-A streptococcal virulence.

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1986-11-01
2024-11-02
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References

  1. Björck Kronvall G. 1984; Purification and some properties of streptococcal protein G. a novel IgG-binding reagent. Journal of Immunology 133:969–974
    [Google Scholar]
  2. Burova L. A. 1980; Changes in virulence, M protein and IgG Fc-receptor activity in a type 12 group A streptococcal strain during mouse-passages. Acta Pathologica Microhiologica Scanilinavica Section B 88:199–205
    [Google Scholar]
  3. Burova L. A. 1982; Streptococcal IgG Fc-receptor as a virulence factor. In Holm S. E., Christensen P. Basic concepts of streptococci and streptococcal diseases Reedbooks Ltd, Fox Lane North; Chertsey, Surrey. England: pp 205–206
    [Google Scholar]
  4. Burova L. A. 1985; Anti-immunoglobulins in experimental streptococcal immunization: relation to bacterial growth conditions and Fc-receptors. Acta Pathologica Microhiologica Immunologica Scandinavica Section C 93:19–23
    [Google Scholar]
  5. Christensen Svensson M. L. 1979; Demonstration of the non-identity between the Fc receptor for human IgG from group A streptococci type 15 and M protein peptidoglycan and the group specific carbohydrate. Ada Pathologica Mtcrobiologica Scandinavica Section C 87:257–261
    [Google Scholar]
  6. Christensen Holm S. E. 1976; Purification of immunoglobulin G Fc-reactive factor from Streptococcus azgazardah . Acta Pathologica Microhiologicu Scandinavica Section C 84:196–202
    [Google Scholar]
  7. Christensen Johansson B. G., Kronvall G. 1976; Interaction of streptococci with the Fc-fragment of IgG. Acta Pathologica Microhiologicu Scandinavica Section C 84:73–76
    [Google Scholar]
  8. Christensen P., Oxelius V. A. 1974; Quantitation of the uptake of human IgG by some streptococci groups, A. B. C and G. Acta Pathologica Microbiologica Scandinavica Section B 82:475–483
    [Google Scholar]
  9. Fischer G. W., Lowell G. H., Crumrine M. H., Bass J. W. 1978; Demonstration of opsonic activity and in vivo protection against group B streptococci type III by Streptococcus pneumoniae type 14 anti-sera. Journal of Experimental Medicine 148:776–786
    [Google Scholar]
  10. Galfre Howe S. C., Milstein C., Butcher G. W., Howard J. C. 1977; Antibodies to major histocompatibility antigens produced by hybrid cellmis. Nature 266:550–552
    [Google Scholar]
  11. Grubb A., Christensen P., Schalén C. 1982; Isolation and some properties of an IgG Fc-binding protein from group A streptococci type 15. International Archives of Allergy and Applied Immunology 67:369–376
    [Google Scholar]
  12. Kronvall Williams R. C. 1969; Differences in anti-protein A activity among IgG subgroups. Journal of Immunology 103:828–833
    [Google Scholar]
  13. Lancefield R. C. 1954; Cellular constituents of group A streptococci concerned in antigenecity and virulence. In McCarty M. (ed) Streptococcal infections Columbia University Press; New York: pp 3–18
    [Google Scholar]
  14. Lebrun L., Pillot J., Grangeot-Keros L. 1982; Significance of anti-IgG antibodies obtained by immunization of rabbits with some streptococcal strains. Annales d’Immunologie (Institut Pasteur) 133C:45–56
    [Google Scholar]
  15. Löfberg H., Grubb A. 1979; Quantitation of γ-trace in human biological fluids: indication for production in the central nervous system. Scandinavian Journal of Clinical Laboratory Investigation 39:619–626
    [Google Scholar]
  16. McConahey P. J., Dixon F. J. 1966; A method of trace iodination of proteins for immunologic studies. International Archives of Allergy and Applied Immunology 29:185–189
    [Google Scholar]
  17. Myhre E. B., Kronvall G. 1977; Heterogeneity of nonimmune immunoglobulin in Fc reactivity among gram-positive cocci: description of three major types of receptors for human immunoglobulin G. Infection and Immunity 17:475–482
    [Google Scholar]
  18. Reis K. J., Ayoub E. M., BoyleM D. P. 1984a; Streptococcal Fc receptors. I. Isolation and partial characterization of the receptor from a group C streptococcus. II. Comparison of the reactivity of a receptor from a group C streptococcus with staphylococcal protein A. Journal of Immunology 132:3091–3097
    [Google Scholar]
  19. Reis K. J., Boyle M. D. P., Ayoub E. M. 1984b; Identification of distinct Fc-receptor molecules on streptococci and staphylococci. Journal of Clinical and Laboratory Immunology 13:75–80
    [Google Scholar]
  20. Reis K. J., Yarnall M., Ayoub E. M., BoyleM D. P. 1984c; Effect of mouse passage on Fc receptor expression by group A streptococci. Scandinavian Journal of Immunology 20:433–439
    [Google Scholar]
  21. Schalén C., Christensen P., Grubb A., Samuelsson G., Svensson M. L. 1980; Demonstration of separate receptors for human IgA and IgG in group A streptococci type 4. Separation of the solubilized receptors from group- and type-specific antigens, lipoteichoic acid and peptidoglycan. Acta Pathologica Microbiologica Scandinavica Section C 88:77–82
    [Google Scholar]
  22. Schalén C., Burova L. A., Christensen P., Grubb R., Svensson M. L. 1981; IgG F(ab)2 of rabbit anti-M sera but not the unfractionated sera are bactericidal for some group A streptococci with IgG Fc-receptor activity: opsonic effects ascribable to anti-IgG. Acta Pathologica Microbiologica Scandinavica Section C 89:247–252
    [Google Scholar]
  23. Schalén C., Zätterström U., Svensson M. L., Christensen P. 1983; Binding of aggregated IgG Fab-fragments and light chains to some group, A. group C and group G streptococci. Acta Pathologica Microbiologica Scandinavica Section B 91:27–33
    [Google Scholar]
  24. Shulman M., Wilde C. D., Köhler G. 1978; A better cell line for making hybridomas secreting specific antibodies. Nature 276:269–210
    [Google Scholar]
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