1887

Abstract

Summary

A soluble haemagglutinin has been identified in cell-free culture supernates of human diarrhoeal isolates of and It was oligomeric; a major peak of haemagglutinating activity had an apparent mol. wt of 780000 but there was haemagglutinating activity throughout the mol. wt range <40000— > 10. Human group O, A and B, horse, rabbit, chicken and rat erythrocytes, but not those of sheep and cow, were agglutinated by the soluble haemagglutinin, in contrast to the cell-bound agglutinin. Agglutination was inhibited by fetuin, a complex glycoprotein, but not by simple sugars. The haemagglutinating activity was not affected by 0·5 NaCl, dithiothreitol or the presence or absence of Ca. It was unrelated to the haemolytic, enterotoxigenic and proteolytic activities present in cell-free extracts of All , 73% of and 68% of strains tested produced this soluble haemagglutinin. does not appear to be an enteric pathogen, therefore this soluble haemagglutinin alone is unlikely to be a virulence factor in spp.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-21-4-319
1986-06-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/21/4/medmicro-21-4-319.html?itemId=/content/journal/jmm/10.1099/00222615-21-4-319&mimeType=html&fmt=ahah

References

  1. Asao T., Kinoshita Y., Kozaki S., Uemura T., Sakaguchi G. 1984; Purification and some properties of Aeromonas hydrophila hemolysin. Infection and Immunity 46:122–127
    [Google Scholar]
  2. AskelÖf P., Granstrom M., Gillenius P., Lindberg A A. 1982; Purification and characterisation of a fimbrial haemagglutinin from Bordetella pertussis for use in an enzyme-linked immunosorbent assay. Journal of Medical Microbiology 15:73–83
    [Google Scholar]
  3. Atkinson H M., Trust T J. 1980; Hemagglutination properties and adherence ability of Aeromonas hydrophila. Infection and Immunity 27:938–946
    [Google Scholar]
  4. Bernheimer A W., Avigad L S. 1974; Partial characterization of aerolysin, a lytic exotoxin from Aeromonas hydrophila. Infection and Immunity 9:1016–1021
    [Google Scholar]
  5. Buckley J T., Halasa L N., Lund K D., MacIntyre S. 1981; Purification and some properties of the hemolytic toxin aerolysin. Canadian Journal of Biochemistry 59:430–435
    [Google Scholar]
  6. Burke V., Cooper M., Robinson J., Gracey M., Lesmana M., Echeverria P., Janda J M. 1984a; Hemagglutination patterns of Aeromonas spp. in relation to biotype and source. Journal of Clinical Microbiology 19:39–43
    [Google Scholar]
  7. Burke V., Robinson J., Atkinson H M., Dibley M., Berry R J., Gracey M. 1981a; Exotoxins of Aeromonas hydrophila. Australian Journal of Experimental Biology and Medical Science 59:753–761
    [Google Scholar]
  8. Burke V., Robinson J., Berry R J., Gracey M. 1981b; Detection of enterotoxins of Aeromonas hydrophila by suckling-mouse test. Journal of Medical Microbiology 14:401–408
    [Google Scholar]
  9. Burke V., Robinson J., Cooper M., Beaman J., Partridge K., Peterson D., Gracey M. 1984b; Biotyping and virulence factors in clinical and environmental isolates of Aeromonas species. Applied and Environmental Microbiology 47:1146–1149
    [Google Scholar]
  10. Chang B J., Lawson M A., Silburn K A. 1985; Adhesive and invasive properties of Aeromonas hydrophila. Australian Microbiologist 6:193
    [Google Scholar]
  11. Finkelstein R A., Hanne L F. 1983; Hemagglutinins (colonization factors?) produced by Vibrio cholerae.. In Kuwahara S., Pierce N F. eds Advances in research on cholera and related diarrheas. Proceedings of the 17th Joint Conference on Cholera. Nijhoff; Boston: pp 121–125
    [Google Scholar]
  12. Finkelstein R A., Boesman-Finkelstein M., Holt P. 1983; Vibrio cholerae hemagglutinin/lectin/protease hydrolyzes fibro- nectin and ovomucin: F M Burnet revisited. Proceedings of the National Academy of Sciences of the USA 80:1092–1095
    [Google Scholar]
  13. Hośtácka A., Ciženar I., Korych B., Karolček J. 1982; Toxic factors of Aeromonas hydrophila and Plesiomonas shigelloides. Zentralblat fur Bakteriologie. Mikrobiologie und Hygiene I. Abt. Orig. A 252:525–534
    [Google Scholar]
  14. Oishi K., Yokoshima S., Tomiyama T., Aida K. 1979; Exohemagglutinins; New Products of Vibrios. Applied and Environmental Microbiology 38:169–172
    [Google Scholar]
  15. Popoff M. 1984; Aeromonas Kluyver and Van Niel 1936, 398AL. In Krieg N R., Holt J G. eds Bergey’s manual of systematic bacteriology. vol 1: Williams and Wilkins; Baltimore: pp 545–548
    [Google Scholar]
  16. Satterwhite T K., Evans D G., Dupont H L., Evans D J. 1978; Role of Escherichia coli colonisation factor antigen in acute diarrhoea. Lancet 2:181–184
    [Google Scholar]
  17. Sokol P A., Ohman D E., Iglewski B H. 1979; A more sensitive plate assay for detection of protease production by Pseudomonas aeruginosa. Journal of Clinical Microbiology 9:538–540
    [Google Scholar]
  18. Watson I M., Robinson J O., Burke V., Gracey M. 1985; Invasiveness of Aeromonas spp. in relation to biotype, virulence factors and clinical features. Journal of Clinical Microbiology 22:48–51
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-21-4-319
Loading
/content/journal/jmm/10.1099/00222615-21-4-319
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error