1887

Abstract

SUMMARY

Whole-cell rhamnose concentrations were measured in 48 strains of streptococci resembling and . Physiological characteristics were tested by the API-20/Strep system, and it was found that typical (arginine positive, aesculin positive) contained significant amounts of rhamnose, while typical (arginine negative, aesculin negative) contained very low or undetectable amounts of rhamnose. Both groups contained dextran-positive and dextran-negative strains. Organisms that were more difficult to speciate (those giving positive results in the arginine or the aesculin test, but not in both) could also be divided into a rhamnose-positive and a rhamnose-negative group; with one exception, all of the rhamnose-positive strains gave a positive result with arginine in the API-20/Strep test.

There were several discrepancies between the results of conventional tests for arginine and aesculin hydrolysis and those of the corresponding API test. The results of conventional tests for arginine hydrolysis did not correlate closely with rhamnose content, and conventional tests for aesculin hydrolysis were less sensitive than API tests. With the API-20/Strep system, can almost always be distinguished from by its ability to hydrolyse arginine.

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1986-05-01
2024-04-26
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References

  1. Andrewes F W, Horder T J. 1906; A study of the streptococci pathogenic for man. Lancet 2:708–713775–782852–855
    [Google Scholar]
  2. Bridge P D, Sneath P H A. 1983; Numerical taxonomy of Streptococcus. Journal of General Microbiology 129:565–597
    [Google Scholar]
  3. Carlsson J. 1968; A numerical taxonomic study of human oral streptococci. Odontologisk Revy 19:137–160
    [Google Scholar]
  4. Colman G. 1969; Transformation of viridans-like streptococci. Journal of General Microbiology 57:247–255
    [Google Scholar]
  5. Colman G, Williams R E O. 1965; The cell walls of streptococci. Journal of General Microbiology 41:375–387
    [Google Scholar]
  6. Colman G, Williams R E O. 1972; Taxonomy of some human viridans streptococci. In: Wannamaker L W, Matsen J M. (eds) Streptococci and streptococcal diseases: Recognition, understanding and management. Academic Press; New York: pp 281–299
    [Google Scholar]
  7. Cowan S T. 1974; Cowan and Steel’s Manual for the identification of medical bacteria. , 2nd edn. Cambridge University Press
    [Google Scholar]
  8. Coykendall A L, Munzenmaier A J. 1978; Deoxyribonucleic acid base sequence studies on glucan-producing and glucan- negative strains of Streptococcus mitior. International Journal of Systematic Bacteriology 28:511–515
    [Google Scholar]
  9. Coykendall A L, Specht P A. 1975; DNA base sequence homologies among strainsof Streptococcus sanguis. Journal of General Microbiology 91:92–98
    [Google Scholar]
  10. Cummins C S, Harris H. 1956; The chemical composition of the cell wall in some Gram-positive bacteria and its possible value as a taxonomic character. Journal of General Microbiology 14:583–600
    [Google Scholar]
  11. Facklam R R. 1977; Physiological differentiation of viridans streptococci. Journal of Clinical Microbiology 5:184–201
    [Google Scholar]
  12. Hamada S, Torii M, Tsuchitani Y, Kotani S. 1980; Isolation and immunobiological classification of Streptococcus sanguis from human tooth surfaces. Journal of Clinical Microbiology 12:243–249
    [Google Scholar]
  13. Hardie J M, Bowden G H. 1976; Physiological classification of oral viridans streptococci. Journal of Dental Research 55:Suppl 1AA166–A176
    [Google Scholar]
  14. Hare R. 1935; The classification of haemolytic streptococci from the nose and throat of normal human beings by means of precipitin and biochemical tests. Journal of Pathology and Bacteriology 41:499–512
    [Google Scholar]
  15. Menzies I S, Mount J N, Wheeler M J. 1978; Quantitative estimation of clinically important monosaccharides in plasma by rapid thin layer chromotography. Annals of Clinical Biochemistry 15:65–76
    [Google Scholar]
  16. Niven C F, Kiziuta Z, White J C. 1946; Synthesis of a polysaccharide from sucrose by Streptococcus s.b.e. Journal of Bacteriology 51:711–716
    [Google Scholar]
  17. Parker M T, Ball L C. 1976; Streptococci and aerococci associated with systemic infection in man. Journal of Medical Microbiology 9:275–302
    [Google Scholar]
  18. Porterfield J S. 1950; Classification of the streptococci of subacute bacterial endocarditis. Journal of General Microbiology 4:92–101
    [Google Scholar]
  19. Roberts R B, Krieger A G, Schiller N L, Gross K C. 1979; Viridans streptococcal endocarditis: the role of various species, including pyridoxal-dependent streptococci. Reviews of Infectious Diseases 1:955–966
    [Google Scholar]
  20. Roberts W S L, Stewart F S. 1961; The sugar composition of streptococcal cell walls and its relation to haemagglutina- tion pattern. Journal of General Microbiology 24:253–260
    [Google Scholar]
  21. Rosan B. 1976; Relationship of the cell-wall composition of group H streptococci and Streptococcus sanguis to their serological properties. Infection and Immunity 13:1144–1153
    [Google Scholar]
  22. Rosan B, Appelbaum B. 1979; An immunochemical study of Streptococcus sanguis and S.mitis.. In: Parker M T. (ed) Pathogenic streptococci. Reedbooks, Chertsey pp 208–209
    [Google Scholar]
  23. Schotmuller H. 1903; Die Artunterscheidung der fur den Menschenpathogenen Streptokokken durch Blutagar. Münchener Medizinische Wochenschrift 50:849–853909–912
    [Google Scholar]
  24. Slade H D, Slamp W C. 1962; Cell-wall composition and the grouping antigens of streptococci. Journal of Bacteriology 84:345–351
    [Google Scholar]
  25. Waitkins S A, Ball L C, Fraser CAM. 1980; A shortened scheme for the identification of indifferent streptococci. Journal of Clinical Pathology 33:47–52
    [Google Scholar]
  26. Washburn M R, White J C, Niven C F. 1946; Streptococcus s.b.e.: immunological characteristics. Journal of Bacteriology 51:723–729
    [Google Scholar]
  27. White J C, Niven C F. 1946; Streptococcus s.b.e.: a streptococcus associated with subacute bacterial endocarditis. Journal of Bacteriology 51:717–722
    [Google Scholar]
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