1887

Abstract

Summary

Inbred female C3H mice were given 2 × 10 cfu virulent by intraperitoneal injection. Peritoneal washings were harvested between 3 h and 72 h after infection and examined by electronmicroscopy. There was evidence of intracellular killing by polymorphs and macrophages. The degeneration of intracellular salmonellae was seen initially as enlarging central electron-lucent areas in the cytoplasm and peripheral condensation of cytoplasmic granules, followed by disruption of the bacterial envelope and disintegration of cellular structure. Alternatively, the initial injury appeared as an irregular and discontinuous bacterial envelope with compression of the bacterium and diffuse condensation of cytoplasmic granules. It was also evident that virulent salmonellae multiplied extracellularly in the peritoneal cavity of the infected mice.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-21-2-151
1986-03-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/21/2/medmicro-21-2-151.html?itemId=/content/journal/jmm/10.1099/00222615-21-2-151&mimeType=html&fmt=ahah

References

  1. Beckerdite S., Mooney C., Weiss J., Franson R., Elsbach P. 1974; Early and discrete changes in permeability of Escherichia coli and certain other gram-negative bacteria during killing by granulocytes. Journal of Experimental Medicine 140:396–409
    [Google Scholar]
  2. Briles D. E., Lehmeyer J., Forman C. 1981; Phagocytosis and killing of Salmonella typhimurium by peritoneal exudate cells. Infection and Immunity 33:380–388
    [Google Scholar]
  3. Carrol M. E. W., Jackett P. S., Aber V. R., Lowrie D. B. 1979; Phagolysosome formation, cyclic adenosine 3′:5′-mono-phosphate and the fate of Salmonella typhimurium within mouse peritoneal macrophages. Journal of General Microbiology 110:421–429
    [Google Scholar]
  4. Collins F. M. 1974; Vaccines and cell-mediated immunity. Bacteriological Reviews 38:371–402
    [Google Scholar]
  5. Hsu H. S., Mayo D. R. 1973; Interactions between macrophages of guinea pigs and salmonellae. III. Bactericidal action and cytophilic antibodies of macrophages of infected guinea pigs. Infection and Immunity 8:165–172
    [Google Scholar]
  6. Hsu H. S., Piper V. M. 1972; Acquired resistance to and comparative virulence of Salmonella typhimurium demonstrated by cutaneous lesions in guinea pigs. Journal of the Reticuloendothelial Society 11:343–357
    [Google Scholar]
  7. Hsu H. S., Radcliffe A. S. 1968; Interactions between macrophages of guinea pigs and salmonellae. I. Fate of Salmonella typhimurium within macrophages of normal guinea pigs. Journal of Bacteriology 96:191–197
    [Google Scholar]
  8. Mackaness G. B., Blanden R. V., Collins F. M. 1966; Host-parasite relations in mouse typhoid. Journal of Experimental Medicine 124:573–583
    [Google Scholar]
  9. Marecki N. M., Hsu H. S., Mayo D. R. 1975; Cellular and humoral aspects of host resistance in murine salmonellosis. British Journal of Experimental Pathology 56:231–243
    [Google Scholar]
  10. Mayo D. R., Hsu H. S., Lim F. 1977; Interactions between salmonellae and macrophages of guinea pigs. IV. Relationship between migration inhibition and antibacterial action of macrophages. Infection and Immunity 18:52–59
    [Google Scholar]
  11. Mitsuhashi S., Sato I., Tanaka T. 1961; Experimental salmonellosis. Intracellular growth of Salmonella enteritidis ingested in mononuclear phagocytes of mice and cellular basis of immunity. Journal of Bacteriology 81:863–868
    [Google Scholar]
  12. Modrzakowski M. C., Spitznagel J. K. 1979; Bactericidal activity of fractionated granule contents from human polymorphonuclear leukocytes: antagonism of granule cationic proteins by lipopolysaccharide. Infection and Immunity 25:597–602
    [Google Scholar]
  13. Nakoneczna I., Hsu H. S. 1980; The comparative histopathology of primary and secondary lesions in murine salmonellosis. British Journal of Experimental Pathology 61:76–84
    [Google Scholar]
  14. Nakoneczna I., Hsu H. S. 1983; Histopathological study of protective immunity against murine salmonellosis induced by killed vaccine. Infection and Immunity 39:423–430
    [Google Scholar]
  15. Rest R. F., Cooney M. H., Spitznagel J. K. 1978; Bactericidal activity of specific and azurophil granules from human neutrophils: studies with outermembrane mutants of Salmonella typhi-murium LT-2. Infection and Immunity 19:131–137
    [Google Scholar]
  16. Rhodes M. W., Hsu H. S. 1974; The effect of kanamycin on the fate of Salmonella enteritidis within cultured macrophages of guinea pigs. Journal of the Reticuloendothelial Society 15:1–12
    [Google Scholar]
  17. Sato I., Tanaka T., Saito K., Mitsuhashi S. 1962; Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. Journal of Bacteriology 83:1306–1312
    [Google Scholar]
  18. Suter E., Ramseier H. 1964; Cellular reactions in infection. Advances in Immunology 4:117–173
    [Google Scholar]
  19. van Zwet T. L., Thompson J., van Furth R. 1975; Effect of glucocorticosteroids on the phagocytosis and intracellular killing by peritoneal macrophages. Infection and Immunity 12:699–705
    [Google Scholar]
  20. Wells P. S., Hsu H. S. 1970; Interactions between macrophages of guinea pigs and salmonellae. II. Phagocytosis of Salmonella typhimurium by macrophages of normal guinea pigs. Infection and Immunity 2:145–149
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-21-2-151
Loading
/content/journal/jmm/10.1099/00222615-21-2-151
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error