1887

Abstract

Summary

A new and simple method of serotyping campylobacters has been developed which utilises co-agglutination to detect the presence of heat-stable antigens. Campylobacters are heated at 75°C for 30 min to destroy antigenic protein and allowed to react on a glass slide with staphylococci coated with antibody. Of 74 isolates, 67 gave the same result by co-agglutination and the previously described passive haemagglutination method. The co-agglutination technique may be used as a rapid screening test before serotyping by passive haemagglutination.

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/content/journal/jmm/10.1099/00222615-21-1-83
1986-02-01
2022-09-24
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References

  1. Abbott J. D., Dale B., Eldridge J., Jones D. M., Sutcliffe E. M. 1980; Serotyping of Campylobacter jejunijcoli. Journal of Clinical Pathology 33:762–766
    [Google Scholar]
  2. Berg R. L., Jutila J. W., Firehammer B. D. 1971; A revised classification of Vibrio fetus. American Journal of Veterinary Research 32:11–22
    [Google Scholar]
  3. Danielsson D., Oleen P. 1979; Rapid serotyping of groups A, B and C meningococci by rocket-line immunoelectrophoresis and co-agglutination. Journal of Clinical Pathology 32:136–142
    [Google Scholar]
  4. Danielsson D., Kronvall G. 1974; Slide agglutination method for the serological identification of Neisseria gonorrhoeae with anti-gonococcal antibodies adsorbed to protein A-contain-ing staphylococci. Applied Microbiology 27:368–374
    [Google Scholar]
  5. Fricker C. R. 1985; A note on the effect of different storage procedures on the ability of Preston medium to recover Campylobacters. Journal of Applied Bacteriology 58:57–62
    [Google Scholar]
  6. George H. A., Hoffman P. S., Smibert R. M., Krieg N. R. 1978; Improved media for growth and aerotolerance of Campylobacter fetus. Journal of Clinical Microbiology 8:36–41
    [Google Scholar]
  7. Itoh T., Saito K., Yanagawa Y., Sakai S., Ohashi M. 1982 Serological typing of thermophilic Campylobacter isolated in Tokyo. In Newell D. G. (ed) Campylobacter: Epidemiology, pathogenesis and biochemistry MTP Press; Lancaster: pp 106–110
    [Google Scholar]
  8. Kosunen T. U., Danielsson D., Kjellander J. 1980; Serology of Campylobacter fetus ss jejuni (‘related Campylobacters’). Acta Pathologica, Microbiologica el Immunologica Scandi-navica B 88:207–218
    [Google Scholar]
  9. Kronvall G. 1973; A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. Journal of Medical Microbiology 6:187–190
    [Google Scholar]
  10. Lauwers S., Vlaes L., Butzler J. P. 1981; Campylobacter serotyping and epidemiology. Lancet 1:158–159
    [Google Scholar]
  11. Lior H., Woodward D. L., Edgar J. A., Laroche L. J., Gill P. 1982; Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. Journal of Clinical Microbiology 15:761–768
    [Google Scholar]
  12. Mandal B. K., De Mol P., Butzler J. P. 1984 Clinical aspects of Campylobacter infections in humans. In Butzler J. P. (ed) Campylobacter infection in man and animals CRC Press; Florida: pp 21–31
    [Google Scholar]
  13. Morgan W. J. B. 1959; Studies on the antigenic structure of Vibrio fetus. Journal of Comparative Pathology and Therapeutics 69:125–140
    [Google Scholar]
  14. Patton C. M., Barrett T. J., Morris G. K. 1983 Serotyping Campylobacter jejuni/coli by two systems: the CRC experience. In Pearson A. D. et al. (eds) Campylobacter II Public Health Laboratory Service; London: pp 96–97
    [Google Scholar]
  15. Penner J. L., Hennessy J. N. 1980; Passive hemagglutination technique for serotyping Campylobacter fetus subsp jejuni on the basis of soluble heat-stable antigens. Journal of Clinical Microbiology 12:732–737
    [Google Scholar]
  16. Rogol M., Sechter I., Braunstein I., Gerichter C. B. 1982 Provisional antigenic scheme for Campylobacter jejuni. In Newell D. G. (ed) Campylobacter: Epidemiology, pathogenesis and biochemistry MTP Press; Lancaster: pp 98–103
    [Google Scholar]
  17. Skirrow M. B. 1982; Campylobacter enteritis—the first five years. Journal of Hygiene 89:175–184
    [Google Scholar]
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