1887

Abstract

Summary

The protein, polysaccharide and phospholipid constituents of have been partially purified and their immunogenicity studied in mice. Humoral and cellular immune responses were demonstrated against a crude cytoplasmic protein fraction (CPF). Two fractions of CPF were prepared on a Sephadex G-200 column; a high mol. wt fraction, fraction-1(F) was capable of eliciting both types of immune responses, whereas fraction-2(F) behaved more like a hapten. Phosphatides elicited only humoral responses whereas polysaccharides were non-immunogenic.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-20-2-263
1985-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/20/2/medmicro-20-2-263.html?itemId=/content/journal/jmm/10.1099/00222615-20-2-263&mimeType=html&fmt=ahah

References

  1. Banerjee B., Subrahmanyam D. 1978; Antigenicity of the phosphatidyl inositomannosides of Mycobacteria. Immunochemistry 15:359–363
    [Google Scholar]
  2. Bartlett G. R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234:466–468
    [Google Scholar]
  3. Beaman B. L., Gershwin M. E., Ahmed A., Scates S. M., Deem R. 1982; Response of CBA/N xDBA2/Fi mice to Nocardia asteroides. Infection and Immunity 35:111–116
    [Google Scholar]
  4. Beaman B. L., Maslan S. 1977; Effect of cyclophosphamide on experimental Nocardia asteroides infection in mice. Infection and Immunity 16:995–1004
    [Google Scholar]
  5. Blumer S. O., Kaufman L. 1979; Microimmunodiffusion test for nocardiosis. Journal of Clinical Microbiology 10:308–312
    [Google Scholar]
  6. Bojalil L. F., Zamora A. 1963; Precipitin and skin tests in the diagnosis of mycetoma due to Nocardia brasiliensis. Proceedings of the Society for Experimental Biology and Medicine 113:40–43
    [Google Scholar]
  7. Boyden S. V. 1956; Antigens of Mycobacterium tuberculosis. Advances in Tuberculosis Research 7:17–51
    [Google Scholar]
  8. Curry W. A. 1980; Human nocardiosis: A clinical review with selected case reports. Archives of Internal Medicine 140:818–826
    [Google Scholar]
  9. Daniel T. M., Janicki B. W. 1978; Mycobacterial antigens: a review of their isolation, chemistry and immunological properties. Microbiological Reviews 42:84–113
    [Google Scholar]
  10. Davis B. J. 1964; Disc electrophoresis II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  11. Deem R. L., Beaman B. L., Gershwin M. E. 1982; Adoptive transfer of immunity to Nocardia asteroides in nude mice. Infection and Immunity 38:914–920
    [Google Scholar]
  12. Dubois M., Gilles K. A., Hemilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  13. Falk R. E., Zabriskie J. B. 1971; The capillary technique for measurement of inhibition of leucocyte migration. An assessment of cell-mediated immunity. In Bloom B. R., Glade P. R. eds In vitro methods in cell-mediated immunity. Academic Press; New York: pp 301–306
    [Google Scholar]
  14. Folch J., Lees M., Sloane-Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  15. Herbert W. J. 1978; Passive haemagglutination wth special reference to the tanned cell technique. In Weir D. M. ed Handbook of experimental immunology. , 3rd edn. chapter 20 Blackwell, Oxford:
    [Google Scholar]
  16. Hiramine C., Hojo K., Yano I. 1981; Development of cell-mediated hypersensitivity in guinea pigs following infection with Nocardia asteroides. International Archives of Allergy and Applied Immunology 65:220–234
    [Google Scholar]
  17. Khuller G. K. 1976; The mannophosphoinositides of Nocardia asteroides. Experientia 32:1371–1372
    [Google Scholar]
  18. Khuller G. K., Chopra A. 1983; Mannophosphoinositides of actinomycetales. Actinomycetes 17:22–49
    [Google Scholar]
  19. Khuller G. K., Penumarti N., Subrahmanyam D. 1983; Induction of resistance to tuberculosis in guinea pigs with mannophosphoinositides of mycobacteria. IRCS Medical Science 11:288–289
    [Google Scholar]
  20. Khuller G. K., Subrahmanyam D. 1971; Antigenicity of phosphatidyl inositomannosides of Mycobacterium tuberculosis. Immunochemistry 8:251–256
    [Google Scholar]
  21. Kotani S. et al. 1983; Immunobiological activities of synthetic lipid A analogs and related compounds as compared with those of bacterial lipopolysaccharide, re-glycolipid, lipid A and muramyl dipeptide. Infection and Immunity 41:758–773
    [Google Scholar]
  22. Krick J. A., Remington J. S. 1975; Resistance to infection with Nocardia asteroides. Journal of Infectious Diseases 131:665–672
    [Google Scholar]
  23. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  24. Ortiz-Ortiz L., Bojalil L. F., Contreras M. F. 1972; Delayed hypersensitivity to polysaccharides from Nocardia. Journal of Immunology 108:1409–1413
    [Google Scholar]
  25. Palmer D. L., Harvey R. L., Wheeler J. K. 1974; Diagnosis and therapeutic considerations in Nocardia asteroides infection. Medicine (Baltimore) 53:391–401
    [Google Scholar]
  26. Shainhouse J. Z., Pier A. C., Stevens D. A. 1978; Complement fixation antibody test for human nocardiosis. Journal of Clinical Microbiology 8:516–519
    [Google Scholar]
  27. Sundararaj T., Agarwal S. C. 1977; Cell-mediated immunity to Nocardia asteroides induced by its ribonucleic acid protein fraction. Infection and Immunity 18:253–256
    [Google Scholar]
  28. Takahashi Y. 1962; Specific serum agglutination of Kaolin particles sensitized with tubercle phosphatide and its clinical evaluation as a serodiagnostic test for tuberculosis. American Review of Respiratory Diseases 85:708–719
    [Google Scholar]
  29. Zamora A., Bojalil L. F., Bastarrachea F. 1963; Immunologically active polysaccharides from Nocardia asteroides and Nocardia brasiliensis. Journal of Bacteriology 85:549–555
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-20-2-263
Loading
/content/journal/jmm/10.1099/00222615-20-2-263
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error