1887

Journal of Medical Microbiology logo

Volume 20, Issue 1

Evolution of Austalian Isolates of Methicillin-Resistant : a Problem of Plasmid Incompatibility? Free

Abstract

Summary

Methicillin-resistant (MRSA), currently causing problems in Australian hospitals, have chromosomal penicillinase and carry a new family of incompatibility group I (IncI) plasmids that encode resistance to nucleic acid-binding compounds (NAB). These plasmids may carry additional determinants for penicillinase production and resistance to gentamicin and trimethoprim. By comparison, earlier MRSA isolates from Australia were NAB-sensitive and the penicillinase determinants were carried on IncI plasmids. The possibility that these newer MRSA isolates have the same ‘clonal’ origin as other MRSA isolates has been investigated. Forcible maintenance of IncI penicillinase plasmids and NAC-resistance plasmids in the same cells resulted in various recombination events. Similar recombination events to those generated in the laboratory have been found in MRSA isolates.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology, 1985
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-20-1-49
1985-08-01
2023-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/20/1/medmicro-20-1-49.html?itemId=/content/journal/jmm/10.1099/00222615-20-1-49&mimeType=html&fmt=ahah

References

  1. Annear D. I., Grubb W. B. 1972; Unstable resistance to kanamycin, lincomycin and penicillin in a methicillin resistant culture of Staphylococcus aureus. Pathology 4:247–252
     [Google Scholar]
  2. Dornbusch K. 1973; Compatibility tests between genetic linkage groups in a strain of Staphylococcus aureus. Journal of General Microbiology 76:13–19
     [Google Scholar]
  3. Duggleby R. G., Kinns H., Rood J. I. 1981; A computer program for determining the size of DNA restriction fragments. Analytical Biochemistry 110:49–55
     [Google Scholar]
  4. Dyke K. G. H. 1969; Penicillinase production and intrinsic resistance to penicillins in methicillin-resistant cultures of Staphylococcus aureus. Journal of Medical Microbiology 2:261–278
     [Google Scholar]
  5. Emslie K. R., Townsend D. E., Grubb W. B. 1985; A resistance determinant to nucleic acid-binding compounds in methicillin-resistant Staphylococcus aureus. Journal of Medical Micriobiology 20: In press
    [Google Scholar]
  6. Emslie K. R., Townsend D. E., Grubb W. B. Two distinct resistance determinants to nucleic acid-binding compounds in Staphylococcus aureus? FEMS Microbiology Letters In press..
    [Google Scholar]
  7. Gedney J., Lacey R. W. 1982; Properties of methicillin-resistant staphylococci now endemic in Australia. The Medical Journal of Australia 1:448–450
     [Google Scholar]
  8. Grubb W. B., O’Reilly R. J. 1971; Joint transduction of separate extrachromosomal drug resistance determinants in Staphylococcus aureus El69. Biochemical and Biophysical Research Communications 42:377–383
     [Google Scholar]
  9. Grubb W. B., Townsend D. E., Greed L. C., Ashdown N., Momoh M. 1983; Characteristics of methicillin-resistant Staphylococcus aureus endemic in Australian hospitals. In Spitzy KH., Karrer K. eds Proceedings of 13th International Congress of Chemotherapy, Vienna. Verlag H. Egermann Pt; 8323–26
     [Google Scholar]
  10. Iordanescu S., Surdeanu M. 1980; New incompatiblity groups for Staphylococcus aureus plasmids. Plasmid 4:256–260
     [Google Scholar]
  11. Iordanescu S., Surdeanu M., Della Latta P., Novick R. 1978; Incompatibility and molecular relationships between small staphylococcal plasmids carrying the same resistance marker. Plasmid 1:468–479
     [Google Scholar]
  12. Kigbo E. P., Townsend D. E., Ashdown N., Grubb W. B. Transposition of penicillinase determinants in methicillin-resistant Staphylococcus aureus. FEMS Microbiology Letters In press
    [Google Scholar]
  13. Lacey R. W. 1972; Genetic control in methicillin-resistant strains of Staphylococcus aureus. Journal of Medical Microbiology 5:497–508
     [Google Scholar]
  14. Lacey R. W. 1975; Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. Bacteriological Reviews 39:1–32
     [Google Scholar]
  15. Lacey R. W., Grinsted J. 1973; Genetic analysis of methicillin-resistant strains of Staphylococcus aureus; evidence for their evolution from a single clone. Journal of Medical Microbiology 6:511–526
     [Google Scholar]
  16. Lyon B. R., Iuorio J. L., May J. W., Skurray R. A. 1984; Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. Journal of Medical Microbiology 17:79–89
     [Google Scholar]
  17. Lyon B. R., May J. W., Marshall J. H., Skurray R. A. 1982; Plasmid-mediated antibiotic resistance in methicillin-resistant Staphylococcus aureus. The Medical Journal of Australia 1:468–469
     [Google Scholar]
  18. Lyon B. R., May J. W., Skurray R. A. 1983; Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 23:817–826
     [Google Scholar]
  19. McDonald P. J. 1982; Methicillin-resistant staphylococci. A sign of the times?. The Medical Journal of Australia 1:445–446
     [Google Scholar]
  20. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. 1979; Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid 2:109–129
     [Google Scholar]
  21. Novick R. P. 1969; Extrachromosomal inheritance in bacteria. Bacteriological Reviews. 33:210–263
     [Google Scholar]
  22. Novick R. P. 1979; The molecular basis of the plasmid state. In Hertman I. ed Extrachromosomal inheritance in bacteria; Contributions to Microbiology and Immunology, no. 6. Karger, Basel, Switzerland: pp 1–15
     [Google Scholar]
  23. Novick R. P., Bouanchaud D. 1971; Extrachromosomal nature of drug resistance in Staphylococcus aureus. Annals of the New York Academy of Science 182:279–294
     [Google Scholar]
  24. Novick R. P., Brodsky R. 1972; Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus. Journal of Molecular Biology 68:285–302
     [Google Scholar]
  25. Novick R. P., Hoppensteadt F. C. 1978; On plasmid incompatibility. Plasmid 1:421–434
     [Google Scholar]
  26. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. 1979; Penicillinase plasmids of Staphylococcus aureus: restriction-depletion maps. Plasmid 2:109–129
     [Google Scholar]
  27. Novick R. P., Richmond M. H. 1965; Nature and interactions of the genetic elements governing penicillinase synthesis in Staphylococcus aureus. Journal of Bacteriology 90:467–480
     [Google Scholar]
  28. Pavillard R. et al. 1982; Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in Victorian hospitals. The Medical Journal of Australia 1:451–454
     [Google Scholar]
  29. Peyru G., Wexler L. F., Novick R. P. 1969; Naturally occurring penicillinase plasmids in Staphylococcus aureus. Journal of Bacteriology 98:215–221
     [Google Scholar]
  30. Ruby C., Novick R. P. 1975 Plasmid interactions in Staphylococcus aureus: Nonadditivity of compatible plasmid DNA pools. Proceedings of the National Academy of Science; USA: 725031–5035
     [Google Scholar]
  31. Schwesinger M. D., Novick R. P. 1975; Prophage-dependent plasmid integration in Staphylococcus aureus. Journal of Bacteriology 123:724–738
     [Google Scholar]
  32. Shalita Z., Murphy E., Novick R. P. 1980; Penicillinase plasmids of Staphylococcus aureus: structural and evolutionary relationships. Plasmid 3:291–311
     [Google Scholar]
  33. Townsend D. E., Ashdown N., Greed L. C., Grubb W. B. 1984a; Analysis of plasmids mediating gentamicin resistance in methicillin-resistant Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 13:347–352
     [Google Scholar]
  34. Townsend D. E., Ashdown N., Greed L. C., Grubb W. B. 1984b; Transposition of gentamicin resistance to staphylococcal plasmids encoding resistance to cationic agents. Journal of Antimicrobial Chemotherapy 14:115–124
     [Google Scholar]
  35. Townsend D. E., Greed L. C., Ashdown N., Grubb W. B. 1983a; Plasmid-mediated resistance to quaternary ammonium compounds in methicillin-resistant Staphylococcus aureus. The Medical Journal of Australia 2:310
     [Google Scholar]
  36. Townsend D. E., Grubb W. B., Ashdown N. 1983b; Gentamicin resistance in methicillin-resistant Staphylococcus aureus. Pathology 15:169–174
     [Google Scholar]
  37. Townsend D. E., Grubb W. B., Ashdown N. 1983c; Genetics of drug resistance in methicillin-resistant Staphylococcus aureus from Australian hospitals. Journal of Hospital Infection 4:331–337
     [Google Scholar]
  38. Townsend D. E., Grubb W. B., Ashdown N., Annear D. I., Pearman J. W. 1984c; Genetic analysis of methicillin-resistant Staphylococcus aureus from a Western Australian hospital. Journal of Hospital Infection 5:417–424
     [Google Scholar]
  39. Wyman L., Novick R. P. 1974; Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid. Molecular and General Genetics 135:149–161
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-20-1-49
Loading
Evolution of Austalian Isolates of Methicillin-Resistant Staphylococcus Aureus: a Problem of Plasmid Incompatibility?
J. Med. Microbiol. 20, 49 (1985); https://doi.org/10.1099/00222615-20-1-49
/content/journal/jmm/10.1099/00222615-20-1-49
/content/journal/jmm/10.1099/00222615-20-1-49
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error