1887

Abstract

. Group-B streptococci that possess a type-specific surface polysaccharide undergo phagocytosis only in the presence of antibodies to this, and complement. The snail forms a lectin that is specific for sialic acid; treatment with this promoted the phagocytosis of a group-B streptococcus of serotype Ia (strain O90) in the absence of opsonic antibodies. The effect of the lectin was dose-dependent and required the presence of complement. The specificity of the lectin reaction for sialic acid was proved by the inhibition of phagocytosis by bovine submaxillary mucin. The participation of complement in the reaction was confirmed by demonstrating that C3 was bound to the surface of lectin-treated cells.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-19-3-317
1985-06-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/19/3/medmicro-19-3-317.html?itemId=/content/journal/jmm/10.1099/00222615-19-3-317&mimeType=html&fmt=ahah

References

  1. Aminoff D. 1961; Methods for the quantitative estimation of N-acetylneuraminic acid and theirapplication to hydrolysates of sialomucoids. Biochemical Journal 81:384–392
    [Google Scholar]
  2. Baker C J, Kasper D L. 1976; Identification of sialic acid in polysaccharide antigens of group B Streptococcus. Infection and Immunity 13:284–288
    [Google Scholar]
  3. Baltimore R S, Kasper D L, Baker C J, Goroff D K. 1977; Antigenic specificity of opsonophagocytic antibodies in rabbit antisera to group B streptococci. Journal of Immunology 118:673–678
    [Google Scholar]
  4. Barry G T, Goebel W F. 1957; Colominic acid, a substance of bacterial origin related to sialic acid. Nature 179:206
    [Google Scholar]
  5. Bhattacharjee A K, Jennings H J, Kenny C P, Martin A, Smith I C P. 1975; Structuraldetermination of the sialic acid polysaccharide antigens of Neisseria meningitidis sero- groups B and C with carbon 13 nuclear magnetic resonance. Journal of Biological Chemistry 250:1926–1932
    [Google Scholar]
  6. Edwards M S, Kasper D L, Jennings H J, Baker C J, Nicholson-Weller A. 1982; Capsular sialicacid prevents activation of the alternative complement pathway by type III, group Bstreptococci. Journal of Immunology 128:1278–1283
    [Google Scholar]
  7. Edwards M S, Nicholson-Weller A, Baker C J, Kasper D L. 1980; The role of specific antibody in lternative complement pathway-mediated opsonophagocytosis of type III, group B Streptococcus. Journal of Experimental Medicine 151:1275–1287
    [Google Scholar]
  8. Holm S E, Falsen E. 1967; An antigen-free medium for cultivation of beta-hemolytic streptococci. Ada Pathologica et Microbiologica Scandinavica 69:264–276
    [Google Scholar]
  9. Jennings H J, Katzenellenbogen E, Lugowski C, Kasper D L. 1983a; Structure of nativepolysaccharide antigens of type la and type lb group B Streptococcus . Biochemistry 22:1258–1264
    [Google Scholar]
  10. Jennings H J, Roseli K-G, Kasper D L. 1980; Structural determination and serology of the nativepolysaccharide antigen of type-III group B Streptococcus . Canadian Journal of Biochemistry 58:112–120
    [Google Scholar]
  11. Jennings H J, Roseli K-G, Katzenellenbogen E, Kasper D L. 1983b; Structural determination of the capsular polysaccharide antigen of type II group BStreptococcus . Journal of Biological Chemistry 258:1793–1798
    [Google Scholar]
  12. Kerby G P. 1950; A comparison of the removal of mucoid and non-mucoid variants of Klebsiella pneumoniae type b from the splanchnic circulating blood of the intact animal. Journal of Immunology 64:131–137
    [Google Scholar]
  13. Lancefield R C, McCarty M, Everly W N. 1975; Multiple mouse-protective antibodies directedagainst group B streptococci. Special reference to antibodies effective against protein antigens. Journal of Experimental Medicine 142:165–179
    [Google Scholar]
  14. Liu T Y, Gotschlich E C, Dunne F T, Jonssen E K. 1977; Studies on the meningococcalpolysaccharides. Composition and chemical properties of the group B and C polysaccharide. Journal of Biological Chemistry 246:4703–4712
    [Google Scholar]
  15. Lòfgren S, Tàrnvik A, Bloom G D, Sjòberg W. 1983; Phagocytosis and killing of Francisella tularensis by human polymorphonuclear leukocytes. Infection and Immunity 39:715–720
    [Google Scholar]
  16. Robbins J B, McCracken G H, Gotschlich E C, Orskov F, Orskov I, Hanson L A. 1974; Escherichia coli K1 capsular polysaccharide associated with neonatal meningitis. New England Journal of Medicine 290:1216–1219
    [Google Scholar]
  17. Shigeoka A O, Hall R T, Hemming V G, Allred C D, Hill H R. 1978; Role of antibody andcomplement in opsonization of group B streptococci. Infection and Immunity 21:34–40
    [Google Scholar]
  18. Stevens P, Chu C L, Young L S. 1980; K-1 antigen content and the presence of an additional sialic acid-containing antigen among bacteremic K-1 Escherichia coli: correlation with susceptibility to opsonophagocytosis. Infection and Immunity 29:1055–1061
    [Google Scholar]
  19. Troy F A. 1979; The chemistry and biosynthesis of selected bacterial capsular polymers. Annual Review of Microbiology 33:519–560
    [Google Scholar]
  20. van Furth R, van Zwet T L, Leijh P C J. 1978; In vitro determination of phagocytosis and intracellular killing by polymorphonuclear and mononuclear phagocytes. In: Weir D M(ed) Handbook of experimental immunology. , 3. 32 Blackwell Scientific Publications; Oxford:
    [Google Scholar]
  21. Wagner M. 1982; Agglutination of bacteria by a sialic acid-specific lectin of the snail Cepaea hortensis . Acta histochemica 71:35–39
    [Google Scholar]
  22. Wagner M, Wagner B, Bergholm A-M, Holm S E. Experimental infection with group Bstreptococci in tissue cages implanted in rabbits. Zentralblattfur Bakteriologie Parasiten- kunde Infektionskrankheiten und Hygiene Abt 1 Orig A, in press
    [Google Scholar]
  23. Wagner M, Wagner B, Gunther E. 1982; Interaction of a sialic acid-specific lectin of the snail Cepaea hortensis with group B streptococci. Holm S E, Christensen P. Basic concepts of streptococci and streptococcal diseases Reedbooks Ltd; Chertsey, Surrey:70–71
    [Google Scholar]
  24. Weinstein R, Young L S. 1978; Phagocytic resistance of Escherichia coli K-1 isolates and relationship to virulence. Journal of Clinical Microbiology 8:748–755
    [Google Scholar]
  25. Weller P F, Smith A L, Anderson P, Smith D H. 1977; The role of encapsulation and host age inthe clearance of Haemophilus influenzae bacteremia. Journal of Infectious Diseases 135:34–40
    [Google Scholar]
  26. Wright H D. 1927; Experimental pneumococcal septicaemia and anti-pneumococcal immunity. Journal of Pathology and Bacteriology 30:185–252
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-19-3-317
Loading
/content/journal/jmm/10.1099/00222615-19-3-317
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error