1887

Abstract

Summary

Fastidious anaerobe broth and brain-heart infusion cysteine broth supplemented with ‘Panmede’ (a papain digest of ox liver) 2·5% supported the recovery of five strains from simulated blood cultures. Other media tested in parallel—brain heart infusion cysteine broth without ‘Panmede’ and Brewer’s thiogly-collate broth—were unreliable. Sodium polyanethol sulphonate (Liquoid) 0·05%, inhibited five isolates of , including isolates from patients with Haverhill Fever. Occasionally, Liquoid 0·025% was also inhibitory and a heavy inoculum of one strain, NCTC11194, was completely inhibited by Liquoid 0·012% in simulated nutrient-broth blood cultures. These results suggest that the choice of media included in each blood-culture set is critical for the optimal isolation of . Brain-heart infusion cysteine broth supplemented with ‘Panmede’, or commercially available fastidious anaerobe broth, without Liquoid, is recommended.

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/content/journal/jmm/10.1099/00222615-19-2-181
1985-04-01
2022-11-27
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References

  1. Lambe D W, McPhedran A M, Mertz J A, Stewart P. 1973; Streptobacillus moniliformis isolated from a case of Haverhill fever: biochemical characterization and inhibitory effect of sodium polyanethol sulfonate. American Journal of Clinical Pathology 60:854–60
    [Google Scholar]
  2. Shanson D C. 1974; An experimental assessment of different anaerobic blood culture methods. Journal of Clinical Pathology 27:273–9
    [Google Scholar]
  3. Shanson D C, Gazzard B G, Midgley J, Dixey J, Gibson G L, Stevenson J, Finch R G, Cheesbrough J. 1983; Streptobacillus moniliformis isolated from blood in four cases of Haverhill fever. First outbreak in Britain. Lancet 2:92–94
    [Google Scholar]
  4. Shanson D C, Pratt J. 1983; Effect of adding a papain digest of ox liver to brain heart infusion cysteine broth on the recovery of non-sporing anaerobes from simulated blood cultures. Journal of Clinical Pathology 36:678–82
    [Google Scholar]
  5. Stokes E J. 1974; Blood culture technique. Association of Clinical Pathologists Broadsheet81
    [Google Scholar]
  6. Von Haebler T, Miles A A. 1938; The action of sodium polyanethol sulphonate (‘Liquoid’) on blood cultures. Journal of Pathology and Bacteriology 46:245–252
    [Google Scholar]
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