1887

Abstract

SUMMARY

Five solid media were evaluated for isolation of spp. from faeces: desoxycholate citrate agar (DCA), MacConkey’s agar (MAC), xylose-desoxycholate-citrate agar (XDCA), Rogol’s medium (ROG), which contained ampicillin 20 mg/L and -nitrophenyl-glycer-ine 25 mg/L as inhibitors, and blood agar (BA) with ampicillin 10 mg/L. False negative oxidase tests limited the usefulness of DCA and MAC and, although the use of XDCA avoided the problem of fermentation of lactose, some spp. failed to grow on XDCA or produced minute colonies unsuitable for oxidase tests. BA yielded the highest rate of isolation for spp. from 323 faecal samples—15.2% for all spp. and 9.3% for enterotoxigenic (ENT) strains. This compares with 10.8% for all strains and 6.5% for ENT strains isolated on DCA, 7.1% for all strains and 4% for ENT+ strains on MAC and 4% for all strains and 1.5% for ENT strains on ROG. Blood agar with ampicillin is recommended for isolation of spp. from faeces.

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1984-12-01
2022-05-20
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References

  1. Bailey W. R., Scott E. G. 1974; Diagnostic microbiology. The C V Mosby Company; St Louis, MO:
    [Google Scholar]
  2. Burke V., Robinson J., Atkinson H. M., Gracey M. 1982; Biochemical characteristics of enterotoxigenic Aeromonas spp.. Journal of Clinical Microbiology 15:48–52
    [Google Scholar]
  3. Burke V., Robinson J., Berry R. J., Gracey M. 1981; Detection of enterotoxins of Aeromonas hydrophila by a suckling-mouse test.. Journal of Medical Microbiology 14:401–408
    [Google Scholar]
  4. Cary S. G., Blair E. B. 1964; New transport medium for shipment of clinical specimens. I. Fecal specimens.. Journal of Bacteriology 88:96–98
    [Google Scholar]
  5. Cowan S. T. 1974; Cowan and Steel’s manual for the identification of medical bacteria. The Cambridge University Press; Cambridge:
    [Google Scholar]
  6. Furniss A. L., Lee J. V., Donovan T. J. 1978; The Vibrios. Public Health Laboratory Service Monograph. Series No 11 Her Majesty’s Stationery Office; London:
    [Google Scholar]
  7. Gracey M., Burke V., Robinson J. 1982; Aeromonas-associated gastroenteritis.. Lancet 2:1304–1306
    [Google Scholar]
  8. Havelaar A. H., Hoogendorp C. J., Wesdorp A. J., Scheffers W. A. 1980; False-negative oxidase reaction as a result of medium acidification.. Antonie van Leeuwenhoek, Journal of Microbiology and Serology 46:301–312
    [Google Scholar]
  9. Iveson J. B. 1973; Enrichment procedures for the isolation of Salmonella, Arizona, Edwardsiella and Shigella from faeces.. Journal of Hygiene 71:349–361
    [Google Scholar]
  10. Kaper J., Seidler R. J., Lockman H., Colwell R. R. 1979; Medium for the presumptive identification of Aeromonashydrophila and Enterobacteriaceae.. Applied and Environmental Micro biology 38:1023–1026
    [Google Scholar]
  11. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703
    [Google Scholar]
  12. Martinez-Silva R., Guzmann-Urrego M., Caselitz F. H. 1961; Zur Frage der Bedeutung von Aeromonasstämmen bei Säuglingsenteritis.. Zeitschrift fur Tropenmedizin und Parasitolo-gie 12:445–451 (in German)
    [Google Scholar]
  13. Morris G. K. 1976; Laboratory investigation of diarrhoea in travellers to Mexico: evaluation of methods for detecting enterotoxigenic Escherichia coli. Journal of Clinical Microbiology 3:486–495
    [Google Scholar]
  14. Moulsdale M. T. 1983; Isolation of Aeromonas from faeces. Lancet 1:351
    [Google Scholar]
  15. Overman T. L., Amato R. F., Tomfohrde K. M. 1979; Incidence of “oxidase-variable” strains of Aeromonashydrophila. Journal of Clinical Microbiology 9:244–247
    [Google Scholar]
  16. Richardson C. J. L., Robinson J. O., Wagener L. B., Burke V. 1982; Invitro susceptibility of Aeromonas spp. to antimicrobial agents.. Journal of Antimicrobial Chemotherapy 9:267–274
    [Google Scholar]
  17. Rippey S. R., Cabelli V. J. 1979; Membrane filter procedure for enumeration of Aeromonashydrophila in fresh waters.. Applied and Environmental Microbiology 38:108–113
    [Google Scholar]
  18. Rogol M., Sechter I., Grinberg L., Gerichter C. B. 1979; Pril-xylose-ampicillin agar, a new selective medium for the isolation of Aeromonashydrophila. Journal of Medical Microbiology 12:229–231
    [Google Scholar]
  19. Schubert R. H. W. 1974; Genus II Aeromonas Kluyver and van Neil 1936, 398. In Buchanan R. E., Gibbons N. E. (eds) Bergey’s manual of determinative bacteriology 8th edn Williams and Wilkins; Baltimore, MD: pp 345–348
    [Google Scholar]
  20. Shotts E. B., Rimler R. 1973; Medium for the isolation of Aeromonashydrophila. Applied Microbiology 26:550–553
    [Google Scholar]
  21. Shread P., Donovan T. J., Lee J. V. 1981; A survey of the incidence of Aeromonas in human faeces. Society for General Microbiology Quarterly 8:184
    [Google Scholar]
  22. Trust T. J., Chipman D. C. 1979; Clinical involvement of Aeromonashydrophila. Canadian Medical Association Journal 120:942–946
    [Google Scholar]
  23. VonGraevenitz A., Bucher C. 1983; Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces.. Journal of Clinical Microbiology 17:16–21
    [Google Scholar]
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