1887

Abstract

SUMMARY

The determinant responsible for the ability of spp. to inhibit polymorph phagocytic killing of aerobic organisms has not yet been identified. Therefore, the roles of lipopolysaccharide and capsular polysaccharide of were investigated. Serum-resistant and serum-sensitive strains of were used to indicate inhibition of phagocytic killing and serum killing of aerobes. Whole organisms of , purified lipopolysaccharide and capsular polysaccharide were added to an in-vitro phagocytosis system. Results showed that > 10 bacteroides/ml inhibited both serum and phagocytic killing. Concentrations below 10/ml had little effect on either process. Purified capsular polysaccharide (10 or 100 μg/ml), either alone in the system or in combination with sub-inhibitory concentrations of also markedly inhibited serum and phagocytic killing. Lipopolysaccharide (9 μg/ml) appeared relatively inert. , reputedly non-capsulated, produced identical results to those obtained with , but an encapsulated strain of did not inhibit serum or phagocytic killing.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-17-3-259
1984-06-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/17/3/medmicro-17-3-259.html?itemId=/content/journal/jmm/10.1099/00222615-17-3-259&mimeType=html&fmt=ahah

References

  1. Bjornson A. B., Michael J. G. 1974; Factors in human serum promoting phagocytosis of Pseudomonas aeruginosa. II Interaction of opsonins with the phagocytic cell. Journal of Infectious Diseases 130: Supplement127–131
    [Google Scholar]
  2. Drey wood R. 1946; Qualitative test for carbohydrate material. Industrial and Engineering Chemistry Analytical Edition 18:499
    [Google Scholar]
  3. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. 4th edn Virginia Polytechnic Institute and State University; Blacksburg, VA:
    [Google Scholar]
  4. Ingham H. R., Sisson P. R., Tharagonnet D., Selkon J. B., Codd A. A. 1977; Inhibition of phagocytosis in vitro by obligate anaerobes. Lancet 2:1252–1254
    [Google Scholar]
  5. Ingham H. R., Sisson P. R., Middleton R. L., Narang H. K., Codd A. A., Selkon J. B. 1981; Phagocytosis and killing of bacteria in aerobic and anaerobic conditions. Journal of Medical Microbiology 14:391–399
    [Google Scholar]
  6. Joiner K. A., Hawiger A., Gelfand J. A. 1981; Activation of the alternative complement pathway by blood culture isolates of Bacteroides fragilis. Infection and Immunity 34:303–305
    [Google Scholar]
  7. Jones G. R., Gemmell C. G. 1982; Impairment by Bacteroides species of opsonisation and phagocytosis of Enterobacteria. Journal of Medical Microbiology 15:351–361
    [Google Scholar]
  8. Kasper D. L., Seiler M. W. 1975; Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. Journal of Infectious Diseases 132:440–450
    [Google Scholar]
  9. Kasper D. L. 1976; The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: Immunochemical and morphologic definition. Journal of Infectious Diseases 133:79–87
    [Google Scholar]
  10. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of bactericidal power of the blood. Journal of Hygiene 38:732–749
    [Google Scholar]
  11. Namavar F., Verweij A. M. J. J., Bal M., van Steenbergen T. J. M., de Graaff J., MacLaren D. M. 1983; Effect of anaerobic bacteria on killing of Proteus mirabilis by human polymorphonuclear leukocytes. Infection and Immunity 40:930–935
    [Google Scholar]
  12. Okuda K., Takazoe I. 1973; Antiphagocytic effects of the capsular structure of a pathogenic strain of Bacteroides melaninogenicus. Bulletin of Tokyo Dental College; 14:99–104
    [Google Scholar]
  13. Onderdonk A. B., Bartlett J. G., Louie T., Sullivan-Seigler N., Gorbach S. L. 1976; Microbial synergy in experimental intra-abdominal abscess. Infection and Immunity 13:22–26
    [Google Scholar]
  14. Onderdonk A. B., Kasper D. L., Cisneros R. L., Bartlett J. G. 1977; The capsular polysaccharide of Bacteroides fragilis as a virulence factor: Comparison of the pathogenic potential of encapsulated and unencapsulated strains. Journal of Infectious Diseases 136:82–89
    [Google Scholar]
  15. Tofte R. W., Peterson P. K., Schmeling D., Bracke J., Kim Y., Quie P. G. 1980; Opsonization of four Bacteroides species: role of the classical complement pathway and immunoglobulin. Infection and Immunity 27:784–792
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-17-3-259
Loading
/content/journal/jmm/10.1099/00222615-17-3-259
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error