THE SURVIVAL of anaerobic bacteria during collection, transport and laboratory manipulation of clinical samples is important because the isolation of these bacteria may determine the choice of life-saving antimicrobial treatment. Factors that affect survival include exposure to atmospheric oxygen and light, humidity, temperature, composition of transport media and type of swab, and length of time between collection and culture.

Loesche (1969) introduced the concept of oxygen sensitivity or tolerance and measured the survival of anaerobes on an agar medium exposed to air. Tally (1975) defined oxygen tolerance as the survival time on blood agar in air and Rolfe (1978) expressed oxygen tolerance of bacterial suspensions as the time after inoculation when organisms could not be recovered from suspensions exposed to air at 37°C. Hagen, Wood and Hashimoto (1976) found a decrease of viability of at least 50% in ss. held for 12 h at 4°C aerobically or anaerobically. Justesen and Nielsen (1976) found a recovery rate of 18-76% for spp. and < 1.4% for after exposure to air for 24 h in moist conditions.

The present study was designed to evaluate the relative importance of humidity, temperature, and exposure to oxygen for the survival of anaerobic bacteria of clinical significance.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error