1887

Abstract

Summary

The value of prolonged primary anaerobic incubation for the isolation of anaerobic bacteria from clinical material was assessed by comparison of two incubation systems. Continuous incubation of primary anaerobic plates for 48 h was superior to conventional methods in that more anaerobes were isolated. The results indicate that in some cases prolonged incubation may be the only method of achieving the correct bacteriological diagnosis, particularly when relatively oxygen-sensitive strains are involved or when organisms may be damaged by exposure to oxygen during transport.

Use of extended primary incubation provides a clearer picture of the microbial flora of the original lesion and reveals that some anaerobic genera may be more commonly involved than is at present recognised.

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/content/journal/jmm/10.1099/00222615-13-2-257
1980-05-01
2024-05-06
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References

  1. Baldwin A. W. F. 1975; An improved catalyst sachet for anaerobic jars. Med. lab. Technol 32:329
    [Google Scholar]
  2. Cowan S. T. 1974; Cowan and Steel’s Manual for the identification of medical bacteria. 2nd ed Cambridge University Press; Cambridge:
    [Google Scholar]
  3. Dowell V. R. Jr 1974; Collection of specimens and primary isolation. In Anaerobic bacteria:role in disease edited by Balows A., DeHaan R. M., Dowell V. R. Jr, Guze L. B. Thomas; Springfield: p 9
    [Google Scholar]
  4. Holdeman L. V., Moore W. E. C. 1973 Anaerobe Laboratory manual 2nd ed Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  5. Hollander H. A., Flodstrom A., Holmberg K. 1975; Influence of the collection and transport of specimens on the recovery of bacteria from peritonsillar abscesses. J. clin. Microbiol 2:504
    [Google Scholar]
  6. Sapala J. A., Ponka J. L., Neblett T. R. 1975; The bacteriology of the biliary tract. Henry Ford Hosp. med. J 23:81
    [Google Scholar]
  7. Smith L., De S. 1975 Pathogenic anaerobic bacteria 2nd ed Thomas; Springfield:
    [Google Scholar]
  8. Sonnenwirth A. C. 1974; Incidence of intestinal anaerobes in blood cultures. In Anaerobic bacteria:role in disease edited by Balows A., DeHaan R. M., Dowell V. R. Jr, Guze L. B. Thomas; Springfield: p 157
    [Google Scholar]
  9. Spaulding E. H., Vargo V., Michaelson T. C., Swenson R. M. 1974; A comparison of two procedures for isolating anaerobic bacteria from clinical specimens. In Anaerobic bacteria:role in disease edited by Balows A., DeHaan R. M., Dowell V. R., Guze L. B. Thomas; Springfield: p 37
    [Google Scholar]
  10. Stokes E. J. 1975 Clinical bacteriology 4th rev. ed Arnold; London:
    [Google Scholar]
  11. Sutter V. L., Vargo V., Finegold S. M. 1975 Wadsworth anaerobic bacteriology manual 2nd ed University of California; Los Angeles:
    [Google Scholar]
  12. Watt B. 1972; The recovery of clinically important anaerobes on solid media. J. med. Microbiol 5:211
    [Google Scholar]
  13. Watt B., Collee J. G. 1974; Practical approaches to the isolation and identification of clinically important non-sporing anaerobes. In Infection with non-sporing anaerobic bacteria edited by Phillips I., Sussman M. Livingstone; Edinburgh: p 7
    [Google Scholar]
  14. Watt B., Collee J. G., Brown R. 1976; Tests of performance of anaerobic jars. J. clin. Path 29:534
    [Google Scholar]
  15. Wilkins T., Jimenez-Ulate F. 1975; Anaerobic specimen transport device. J. clin. Microbiol 2:441
    [Google Scholar]
  16. Wren M. W. D. 1977; The culture of clinical specimens for anaerobic bacteria: a comparison of three regimens. J. med. Microbiol 10:195
    [Google Scholar]
  17. Wren M. W. D. 1978; A new selective medium for the isolation of non-sporing anaerobic bacteria from clinical specimens. Med. lab. Sci 35:371
    [Google Scholar]
  18. Wren M. W. D., Baldwin A. W. F., Eldon C. P., Sanderson P. J. 1977a; The anaerobic culture of clinical specimens: a 14 month study. J. med. Microbiol 10:49
    [Google Scholar]
  19. Wren M. W. D., Eldon C. P., Dakin G. H. 1977b; Novobiocin and the differentiation of peptococci and peptostreptococci. J. clin. Path 30:620
    [Google Scholar]
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